Anti-Inflammatory Effect of the Oligomeric Stilbene α-Viniferin and its Mode of the Action through Inhibition of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase

Eun Yong Chung; Byung Hak Kim; Myung Koo Lee; Yeo-Pyo Yun; Seung Ho Lee; Kyung Rak Min; Youngsoo Kim

doi:10.1055/s-2003-42787

Planta Medica, Table of Contents

Planta Med 2003; 69(8): 710-714
DOI: 10.1055/s-2003-42787

Original Paper

Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Anti-Inflammatory Effect of the Oligomeric Stilbene α-Viniferin and its Mode of the Action through Inhibition of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase

Eun Yong Chung¹ , Byung Hak Kim¹ , Myung Koo Lee¹ , Yeo-Pyo Yun¹ , Seung Ho Lee² , Kyung Rak Min¹ , Youngsoo Kim¹

¹College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Korea
²College of Pharmacy, Yeungnam University, Kyeongsan, Korea

This work was financially supported by a grant (KRF-99-042-F00167) from Korea Research Foundation

Abstract

The anti-inflammatory activity of α-viniferin, a trimer of resveratrol, has been demonstrated in an animal model of carrageenin-induced paw edema, and inhibitory effects of the compound on cyclooxygenase (COX) and inducible nitric oxide synthase (iNOS) have been investigated in order to understand the mode of the observed action. α-Viniferin at doses > 30 mg/kg (p. o.) or > 3 mg/kg (i. v.) showed significant anti-inflammatory activity on carrageenin-induced paw edema in mice. α-Viniferin showed an inhibitory effect with an IC₅₀ value of 4.9 μM on COX-2 activity but a very weak inhibitory effect with 55.2 ± 2.1 % of the control (100 %) at 100 μM on COX-1 activity. α-Viniferin at doses of 3 μM to 10 μM inhibited the synthesis of COX-2 transcript in lipopolysaccharide (LPS)-activated murine macrophages Raw264.7. α-Viniferin showed an IC₅₀ value of 2.7 μM on nitric oxide (NO) production in LPS-activated Raw264.7 cells when α-viniferin and LPS were treated simultaneously, but did not inhibit the NO production when α-viniferin was treated at 12 h after LPS stimulation. α-Viniferin inhibited synthesis of iNOS transcript with an IC₅₀ value of 4.7 μM. Consequently, the inhibitory effect of α-viniferin on the release of prostanoids and NO could play an important role to show anti-inflammatory action.

Key words

α-Viniferin - stilbenoids - paw edema - COX-2 - iNOS

Full Text

References

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Prof. Youngsoo Kim, Ph. D.

College of Pharmacy

Chungbuk National University

Cheongju 361-763

Korea

Fax: +82-43-268-2732

Email: youngsoo@cbucc.chungbuk.ac.kr