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Planta Med 2003; 69(12): 1102-1108
DOI: 10.1055/s-2003-45190
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

In Vitro Anti-Inflammatory Activity of Panduratin A Isolated from Kaempferia pandurata in RAW264.7 Cells

Jung-Mi Yun1 , 2 , Hoonjeong Kwon2 , Jae-Kwan Hwang1 , 3
  • 1Bioproducts Research Center, Yonsei University, Seoul, Korea
  • 2Department of Food and Nutrition, Seoul National University, Seoul, Korea
  • 3Department of Biotechnology, Yonsei University, Seoul, Korea
This work was supported by the National Research Lab program through the Functional Biopolymer Lab at Yonsei University (2000-N-NL-01-C-299) and the Korea Science and Engineering Foundation (KOSEF) through the Bioproducts Research Center at Yonsei University
Further Information

Publication History

Received: April 22, 2003

Accepted: October 25, 2003

Publication Date:
29 January 2004 (online)

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Abstract

An active compound identified as panduratin A was isolated from a methanol extract of Kaempferia pandurata (Zingiberaceae). We examined the effect of panduratin A on nitric oxide (NO) and prostaglandin E2 (PGE2) production induced by lipopolysaccharide (LPS) in RAW264.7 cells. Modulations of iNOS and COX-2 enzyme expression were evaluated by Western blotting. Panduratin A strongly inhibited both NO (IC50 : 0.175 μM) and PGE2 (IC50 : 0.0195 μM) production and suppressed both iNOS and COX-2 enzyme expression without any appreciable cytotoxic effect on RAW264.7 cells in a dose-dependent manner. Panduratin A also suppressed the phosphorylation of inhibitor κBα (IκBα) and degradation of IκBα associated with nuclear factor κB (NF-κB) activation. Furthermore, panduratin A inhibited LPS-induced NF-κB transcriptional activity in a dose-dependent manner. These results suggest that panduratin A could exert its inhibitory effects on the production of NO and PGE2 through the suppression of NF-κB activation, indicating its potential for use as an anti-inflammatory agent.

Key words

Kaempferia pandurata - Zingiberaceae - panduratin A - inducible nitric oxide synthase - cyclooxygenase-2 - NF-κB - RAW264.7 cells

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Prof. Jae-Kwan Hwang

Department of Biotechnology

Yonsei University

Seoul 120-749

Republic of Korea

Phone: +82-2-2123-5881

Fax: +82-2-362-7265

Email: jkhwang@yonsei.ac.kr