Abstract
Recently we have isolated four active components from Tanshen (the root of Salvia miltiorrhiza Bunge, Labiatae) responsible for the anti-allergic activities. In this study, the molecular mechanism of action of tanshinones for the inhibition of mast cell degranulation was investigated by testing their effects on the signaling components of the high affinity IgE receptor FcεRI. Activation of FcεRI produced immediate tyrosine phosphorylation of Syk, mitogen-activated protein kinase extracellular signal-regulated kinase, ERK1/ERK2 (p44, p42), and phospholipase Cγ2 (PLCγ2). 5,16-Dihydrotanshinone-I possessed the strongest inhibitory effects on mast cell degranulation and markedly reduced FcεRI-mediated tyrosine phosphorylation of ERK and PLCγ2. This suggests that tanshinones possibly exert their anti-allergic activities by affecting FcεRI-mediated tyrosine phosphorylation of ERK and PLCγ2.
Abbreviations
FcεRI:high affinity IgE receptor
ERK:extracellular signal regulated kinase
PLC: phospholipase C
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Kyeong-Man Kim, Ph. D.
Pharmacology Laboratory
College of Pharmacy
Chonnam National University
Kwang-Ju, 500-757
Korea
Fax: +82-62-530-2949
Email: kmkim@chonnam.ac.kr
