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Horm Metab Res 2004; 36(8): 513-518
DOI: 10.1055/s-2004-825752
Original Basic
© Georg Thieme Verlag Stuttgart · New York

Glucose Catabolic Gene mRNA Levels in Skeletal Muscle Exhibit Non-coordinate Expression in Hyperglycemic Mice

M.  Kato1 , A.  Suwa1 , T.  Shimokawa1
  • 1Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., Tsukuba, Ibaraki, Japan
Further Information

Publication History

Received 11 November 2003

Accepted after Revision 24 February 2004

Publication Date:
24 August 2004 (online)

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Abstract

To assess the correlation between hyperglycemia and glucose catabolic gene levels in diabetic and healthy mice, we determined mRNA levels of pivotal proteins such as glucose transporters, hexokinase II, glycogen synthase, glutamine:fructose-6-phosphate amidotransferase and uncoupling proteins. Both KK and KKAy mice showed marked decreases of Glut1 and Glut4 mRNA levels in soleus compared to C57BL; db/db and ob/ob mice exhibited significantly decreased Glut4 mRNA levels, but not Glut1, in soleus. KK and KKAy mice showed a decrease of soleus HKII gene level, which may indicate decreased intracellular catabolism of glucose. Likewise, GS mRNA level was decreased in soleus muscle tissue in KK and KKAy mice. GFAT mRNA levels was no different between hyperglycemic and normoglycemic mice. In contrast, UCP2 and UCP3 mRNA levels were higher in KK and KKAy mice. Conversely, db/db and ob/ob mice showed a significant decrease in UCP3 mRNA. Individual correlation analysis indicated that the decrease in Glut4 gene levels was only observed in hyperglycemic mice. The more important observation is that the glucose catabolic genes do not exhibit any clear coordinate expression. Abnormal expression of glucose catabolic genes may contribute to hyperglycemia and muscle insulin resistance in these four strains.

Key words

Glucose transporter · Glutamine:fructose-6-phosphate amidotransferase · Glycogen synthase · Hexokinase II · Uncoupling protein

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T. Shimokawa, Ph. D.

Molecular Medicine Laboratories, Institute for Drug Discovery Research

Yamanouchi Pharmaceutical Co., Ltd. · 21 Miyukigaoka · Tsukuba · Ibaraki 305-8585 · Japan

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Email: simokawa@yamanouchi.co.jp