Abstract
The objective of our study was to evaluate the effect of short-term intensive exercise on the susceptibility of serum lipids to ex-vivo peroxidation. We assessed the association between aerobic capacity, serum composition, and serum lipid oxidizability as well as the association between aerobic capacity and the effect of short-term maximal exercise on the kinetics of ex-vivo copper-induced peroxidation of serum lipids. The study involved 30 healthy male volunteers (age 22 - 39 years, BMI 19.4 - 29.8). Following 12-hr fasting, blood was withdrawn for determination of blood lipids, LDL, HDL, and TG, and Vitamin E, and for oxidizability assay of the serum lipids. Subsequently, each volunteer underwent an incremental all-out cardiopulmonary exercise stress test (CPET), performed on a motor-driven treadmill (Quinton Q65, USA). The test protocol was a modified Balke protocol. The results of this test were expressed in terms of mass-dependent maximal oxygen uptake (V·O2max, ml · kg-1 · min-1) and of ventilatory anaerobic threshold (VAT, ml · kg-1 · min-1). Immediately after exercise, blood was re-drawn for the determination of serum Vitamin E and for ex-vivo oxidizibility assay, expressed in terms of maximal absorption of oxidation products (ODmax, absorbance units), maximal rate of their production (Vmax, OD min-1) and the time at which the rate was maximal (tmax, min). Maximal graded exercise had no significant effect on the susceptibility of serum lipids to peroxidation as measured by ODmax (p = 0.38 at 245 nm, and 0.27 at 268 nm),Vmax (p = 0.34 at 245 nm, and 0.49 at 268 nm) and tmax (p = 0.17 at 245 nm, and 0.07 at 268 nm). Also no effect was found on the concentration of serum Vitamin E (p = 0.39). Aerobic capacity was not associated either with the susceptibility of serum lipids to ex-vivo peroxidation or with serum Vitamin E concentration. The present findings indicate that a short graded maximal exercise, lasting 8 - 12 min, is not sufficient to increase the susceptibility of the serum lipids to peroxidation. Thus it may be assumed that the antioxidant capacity of most healthy subjects provides proper protection from a short exhaustive exercise challenge. Also, aerobic capacity in the range represented by our subjects does not seem to influence the susceptibility of serum lipids to peroxidation.
Key words
Lipid peroxidation - free radicals - exercise - aerobic fitness - Vitamin E
References
-
1
Balke B, Ware R W.
An experimental study of physical fitness of Air Force personnel.
US Armed Forces Med J.
1959;
10
675-688
-
2
Bassuk S S, Manson J E.
Physical activity and the prevention of cardiovascular disease.
Curr Atheroscler Rep.
2003;
5
299-307
-
3 Chandan K S, Packer L, Hanninen O. Exercise and Oxygen Toxicity. Amsterdam; Elsevier Science B. V. 1994
-
4
Chandan K S.
Oxidants and antioxidants in exercise.
J Appl Physiol.
1995;
79
675-686
-
5
Child R B, Wilkinson D M, Fallowfield J L, Donnelly A E.
Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to stimulated half-marathon run.
Med Sci Sports Exerc.
1998;
30
1603-1607
-
6
Esterbauer H, Jurgens G.
Mechanistic and genetic aspects of susceptibility of LDL to oxidation.
Curr Opin Lipidol.
1993;
4
114-124
-
7
Esterbauer H, Dieber-Rotheneder M, Stregl G, Waeg G.
Role of Vitamin E in preventing the oxidation of low-density lipoprotein.
Am J Clin Nutr.
1991;
53
314-321
-
8
Ginsburg G S, Agil A, O'Toole M, Rimm E, Douglas P S, Rifai N.
Effects of a single bout of ultraendurance exercise on lipid levels and susceptibility of lipids to peroxidation in triathletes.
JAMA.
1996;
276
221-225
-
9
Groussard C, Rannou-Bekono F, Machefer G, Chevanne M, Vincent S, Sergrent O, Cillard J, Gratase-Delamarche A.
Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise.
Eur J Appl Physiol.
2003;
89
14-20
-
10
Hoffman R M, Garewal H S.
Antioxidants and the prevention of coronary heart disease.
Arch Intern Med.
1995;
155
241-246
-
11
Inbar O, Oren A, Scheinowitz M, Rotstein A, Dlin R, Casaburi R.
Normal cardiopulmonary exercise in 20 to 70-yr-old men.
Med Sci Sports Exerc.
1994;
26
538-546
-
12
Jackson A S, Pollock M L.
Prediction accuracy of body density, lean body weight, and total body volume equations.
Med Sci Sports.
1977;
9
107-115
-
13
Jenkins R R.
Exercise, oxidative stress, and antioxidants: a review.
Int J Sport Nutr.
1993;
3
356-375
-
14
Kanter M M.
Free radicals, exercise, and antioxidant supplementation.
Int J Sport Nutr.
1994;
4
205-220
-
15
Kritharides L, Stocker R.
The use of antioxidant supplements in coronary heart disease.
Atherosclerosis.
2002;
164
211-219
-
16
Kujala U M, Ahoutupa M, Vasankari T, Kaprio J, Tikkanen M J.
Low LDL oxidation in veteran endurance athletes.
Scand J Med Sci Sports.
1996;
6
303-308
-
17
Leaf D A, Kleinman M T, Hamilton M, Barstow T J.
The effect of exercise intensity on lipid peroxidation.
Med Sci Sports Exerc.
1997;
29
1036-1039
-
18
Leaf D A, Kleinman M T, Hamilton M, Deitrick R W.
The Exercise-induced stress paradox: The effects of physical exercise training.
Am J Med Sci.
1999;
317
295-300
-
19
Li L J.
Exercise, oxidative stress, and antioxidants.
Am J Sports Med.
1996;
24
20-24
-
20
Liu M L, Bergholm R, Makimattila S, Lahdenpera S, Valkonen M, Hilden H, Yki-Jarvinen H, Taskinen M R.
A marathon run increases the susceptibility of LDL to oxidation in vitro and modifies plasma antioxidants.
Am J Physiol.
1999;
276
1083-1091
-
21
Margaritis I, Tessier F, Richard M.
Marconnet P. No evidence of oxidative stress after a triathlon race in highly trained competitors.
Int J Sports Med.
1997;
18
186-190
-
22
Marzatico F, Pansarsa O, Bertorelli L, Somenzini Della Valle G.
Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes.
J Sports Med Phys Fitness.
1997;
37
235-239
-
23
Ohkuwa T, Sato Y, Naoi M.
Glutathione and reactive oxygen generation in tissues of young and old exercised rats.
Acta Physiol Scand.
1997;
159
237-244
-
24
Ortenblad N, Madsen K, Djurhuus M S.
Antioxidants status and lipid peroxidation after short-term maximal exercise in trained and untrained humans.
Am J Physiol.
1997;
272
1258-1263
-
25
Parks E J, German J B, Davis P A, Frankel E N, Kappagoda C T, Rutledge J C, Hyson D A, Schneeman B O.
Reduced oxidative susceptibility of LDL from patients participating in an intensive atherosclerosis treatment program.
Am J Clin Nutr.
1998;
68
778-785
-
26 Reznick A Z. Oxidative Stress in Skeletal Muscle. Basel, Switzerland; Birkhauser Verlag 1998
-
27
Ross R.
The pathogenesis of atherosclerosis: a perspective for the 1990s.
Nature.
1993;
362
801-809
-
28
Rush J WE, Sandiford S D.
Plasma glutathione peroxidase in healthy young adults: influence of gender and physical activity.
Clin Biochem.
2003;
36
345-351
-
29
Sacheck J M, Milbury P E, Cannon J G, Roubenoff R.
Effect of vitamin E and eccentric exercise in selected biomarkers of oxidative stress in young and elderly men.
Free Rad Biol Med.
2003;
34
1575-1588
-
30
Sanchez-Quesada J L, Ortega H, Payes-Romero A, Serrat-Serrat J, Gonzalez-Sastre F, Lasuncion M A, Ordonez-Llanos J.
LDL from aerobically trained subjects shows higher resistance to oxidative modification than LDL from sedentary subjects.
Atherosclerosis.
1997;
132
207-213
-
31
Sanchez-Quesada J L, Serradesanferm R H, Serrat-Serrat J, Serra-Grima J R, Gonzalez-Sastre F, Ordonez-Llanos J.
Increase of LDL susceptibility to oxidation occurring after intense, long duration aerobic exercise.
Atherosclerosis.
1995;
118
297-305
-
32
Schnitzer E, Pinchuck I, Fainaru M, Schafer Z, Lichtenberg D.
Copper-induced lipid oxidation in unfractioned plasma: The lag preceding oxidation as a measure of oxidation-resistance.
Biochem Biophys Res Com.
1995;
216
854-861
-
33
Shern-Brewer R, Santanam N, Wetzstein C, White-Welkley J, Parthasarathy S.
Exercise and cardiovascular disease: a new perspective.
Arterioscler Thromb Vasc Biol.
1998;
18
1181-1187
-
34
Shimonov M, Pinchuk I, Bor A, Beigel I, Fainaru M, Rubin M, Lichtenberg D.
Susceptibility of serum lipids to copper-induced peroxidation correlates with the level of high-density lipoprotein cholesterol.
Lipids.
1999;
34
255-259
-
35
Sjodin B, Hellsten Y, Apple F S.
Biochemical mechanisms for oxygen free radical formation during exercise.
Sport Med.
1990;
10
236-254
-
36
Vasankari T J, Kujala U M, Vasankari T M, Ahotupa M.
Reduced oxidized LDL levels after a 10-month exercise program.
Med Sci Sports Exerc.
1998;
30
1496-1501
-
37
Vasankari T J, Kujala U M, Vasankari T M, Vuorimaa T, Ahotupa M.
Effects of acute prolonged exercise on serum and LDL oxidation and antioxidant defenses.
Free Rad Biol Med.
1997;
22
509-513
-
38
Vasankari T J, Kujala U M, Vasankari T M, Vuorimaa T, Ahotupa M.
Increased serum and low-density-lipoprotein antioxidant potential after antioxidant supplementation in endurance athletes.
Am J Clin Nutr.
1997;
65
1052-1056
-
39
Vincent K R, Vincent H K, Braith R W, Lennon S L, Lowenthal D T.
Resistance exercise training attenuates exercise-induced lipid peroxidation in the elderly.
Eur J Appl Physiol.
2002;
87
416-423
-
40
Wetzstein C J, Shern-Brewer R A, Santanam N, Green N R, White-Welkley J E, Parthasarathy S.
Does acute exercise affect the susceptibility of low density lipoprotein to oxidation?.
Free Radic Biol Med.
1998;
24
679-682
O. Inbar
Zinman College, Wingate Institute
Netania
Israel
Phone: + 97254531475
Fax: + 972 98 65 09 60
Email: inbar@macam.ac.il
