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Planta Med 2005; 71(4): 326-330
DOI: 10.1055/s-2005-864098
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of the Antimicrobial Effects of Several Isothiocyanates on Helicobacter pylori

Xavier Haristoy1 , 2 , Jed W. Fahey3 , Isabelle Scholtus2 , Alain Lozniewski1 , 2
  • 1Laboratoire de Bactériologie-Virologie, UMR-CNRS 7565, Faculté de Médecine, Université Henri-Poincaré, Nancy, France
  • 2Laboratoire de Bactériologie, Centre Hospitalier et Universitaire, Nancy, France
  • 3Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, USA
Further Information

Publication History

Received: June 16, 2004

Accepted: December 12, 2004

Publication Date:
27 April 2005 (online)

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Abstract

The antibacterial activity of sulforaphane [4-(methylsulfinyl)butyl isothiocyanate] has been previously described. We have analysed the activities of 12 isothiocyanates (ITC) including sulphoraphane on 25 strains of Helicobacter pylori using an agar dilution assay. In addition, bactericidal effects against H. pylori were determined for the 6 most active ITCs, both directly and against intracellular bacteria in cultured human epithelial (HEp-2) cells. The MIC90 values for these ITCs ranged between 4 and 32 μg/mL and four of the most potent compounds exhibited bactericidal activity against both extra- and intracellular bacteria. Overall, our data indicate that ITCs have a potent antibacterial effect against H. pylori and these naturally occurring phytochemicals might have potential as novel therapeutic agents for H. pylori eradication.

Key words

Helicobacter pylori - isothiocyanate - time-to-kill assay - minimal inhibitory concentration

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Prof. Alain Lozniewski

Laboratoire de Bactériologie

Hôpital Central

29 Avenue du Maréchal de Lattre de Tassigny

54035 Nancy Cedex

France

Phone: +33-3-83-85-18-14

Fax: +33-3-83-85-26-73

Email: a.lozniewski@chu-nancy.fr