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Planta Med 2005; 71(6): 501-507
DOI: 10.1055/s-2005-864149
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Induction of Apoptosis by Panduratin A Isolated from Kaempferia pandurata in Human Colon Cancer HT-29 Cells

Jung-Mi Yun1 , 2 , 3 , Hoonjeong Kwon2 , Hasan Mukhtar3 , Jae-Kwan Hwang1 , 4
  • 1Bioproducts Research Center, Yonsei University, Seoul, Korea
  • 2Department of Food and Nutrition, Seoul National University, Seoul, Korea
  • 3Department of Dermatology, University of Wisconsin, Madison, WI, USA
  • 4Department of Biotechnology, Yonsei University, Seoul, Korea
Further Information

Publication History

Received: August 9, 2004

Accepted: February 13, 2005

Publication Date:
21 June 2005 (online)

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Abstract

We previously showed that panduratin A isolated from an extract of Kaempferia pandurata (Zingiberaceae) was a strong inhibitor of cyclooxygenase-2 (COX-2) in RAW264.7 cells, suggesting a potential use of panduratin A as an anti-inflammatory agent. In the present study, we have investigated the effects of panduratin A on cytoplasmic levels of COX-2, as well as proliferation and apoptosis in human colon cancer cells HT-29. Cell proliferation and induction of apoptosis was determined by the MTT assay, DNA fragmentation measurement, flow cytometric analysis, nuclear staining and Western blotting. The MTT assay indicated that panduratin A exhibited cytotoxicity with an IC50 value of 28 μM. The cytotoxic effects of panduratin A were found to be accompanied by the dose-dependent induction of apoptosis as assessed by DNA fragmentation and apoptotic bodies. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in cleavage of poly(ADP-ribose) polymerase (PARP) with a concomitant decrease in procaspase-3 protein. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in human colon cancer cells, suggesting its potential use as a cancer chemopreventive and therapeutic agent.

Key words

Kaempferia pandurata - Zingiberaceae - panduratin A - caspase-3 - PARP - HT-29 cells

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Prof. Jae-Kwan Hwang

Department of Biotechnology

Yonsei University

Seoul 120-749

Republic of Korea

Phone: +82-2-2123-5881

Fax: +82-2-362-7265

Email: jkhwang@yonsei.ac.kr