Abstract
Glucose-free perfusion preconditions myocardium against the consequences of subsequent ischemia. We investigated whether mitochondrial ATP-sensitive potassium (mKATP) channels are involved in preconditioning by glucose deprivation, and whether moderate glucose deprivation also preconditions myocardium. Isolated rat hearts underwent 30 min of no-flow ischemia followed by 1 h reperfusion. Controls were not further treated. Three groups were preconditioned by perfusion with 0, 40 or 80 mg/dl (0, 2.22, 4.44 mmol/l) glucose (correction of osmotic pressure by addition of urea) for 10 min followed by 10 min perfusion with normal buffer (150 mg/dl, or 8.33 mmol/l glucose) before the ischemia reperfusion protocol. In one group, 100 μmol/l of the mKATP channel blocker 5-HD was added to the glucose-free perfusate. Two groups were treated with 5-HD or urea before ischemia without preconditioning. Left ventricular developed pressure and maximum ischemic contracture (82 ± 21 mmHg) were similar in all groups. Mean left ventricular developed pressure was 100 ± 16 mm Hg under baseline conditions, and poorly recovered to 8 ± 11 mm Hg during reperfusion. Preconditioning with 0 and 40 mg/dl glucose containing buffer reduced infarct size from 41 ± 10 % (control) to 23 ± 12 % (p = 0.02) and 26 ± 8 % (p = 0.011). The 5-HD blocked preconditioning by glucose deprivation (38 ± 9 %, p = 0.04) while 80 mg/dl glucose, 5-HD and urea had no effect on infarct size (39 ± 9 %; 38 ± 13 %; 37 ± 8 %; p = 1.0 each). We conclude that transient severe glucose deprivation and moderate glucose deprivation preconditions the isolated rat heart. Preconditioning by complete glucose deprivation depends on the opening of mKATP channels.
Key words
Rat · Isolated heart · Preconditioning · Glucose · Infarction
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Dr. Dirk Ebel
Klinik für Anästhesiologie, Universitätsklinikum Düsseldorf
Postfach 10 10 07 · 40001 Düsseldorf · Germany
Phone: +49 (211) 81 18101 ·
Fax: +49 (211) 81 16253
Email: ebeld@uni-duesseldorf.de
