Abstract
Six N-benzoylphenylisoserinates of Lactarius sesquiterpenoid alcohols, which previously showed antiviral activities, were tested for their biological properties. Their influence on the mitotic division of the cells and on selected immunological parameters, e. g., T and B lymphocyte proliferation and synthesis of the cytokines: interleukin 2 (IL-2), tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) was assessed in vitro. All of the tested compounds significantly decreased the number of cell divisions. It appears that their influence on cellular divisions may be associated with anti-HSV activity. Moreover, one compound - isolactarorufin 8-epi-[N-benzoyl-(2′R,3′S)-3′-phenylisoserinate] significantly inhibited T lymphocyte proliferation and the synthesis of all tested cytokines.
Abbreviations
DMSO:dimethyl sulphoxide
FCS:foetal calf serum
HEPES:4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid
MEM:minimum essential medium
PBMC:peripheral blood mononuclear cells
PBS:phosphate buffered saline
PHA:phytohaemagglutinin
PMA:phorbol myristate acetate
SAC:Staphylococcus aureus Cowan (strain)
Key words
Sesquiterpenes - taxol -
Lactarius
- mitotic divisions - lymphocyte proliferation - cytokines
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Ewa Krawczyk
Chair and Department of Medical Microbiology
Medical University of Warsaw
5 Chalubinski St.
02-004 Warsaw
Poland
Phone: +48-22-622-0028
Fax: +48-22-628-2739
Email: ewakrawczyk@gazeta.pl
