Abstract
Puberty influences both the performance and the hormonal responses to exercise-related stress. We evaluated the distribution and the correlation between pubertal characteristics and the salivary cortisol (sC) and testosterone (sT) responses to acute physical exercise in young male athletes (13.4 ± 2.1 yr, n = 110). The mean pre-exercise nmol · L-1 sC and sT concentrations were 19.08 ± 4.32 and 0.34 ± 0.15 and increased to 21.27 ± 5.51 and 0.41 ± 0.16 after a 90-min training session (p < 0.01). The sC concentration at rest was positively correlated with chronological age (p < 0.01) and negatively correlated with fat % (p < 0.05), whereas significant negative correlations of ΔsC and ΔsC% after exercise with age, pubertal stage and mean testis volume (p < 0.05) were observed. The sT increase after exercise was correlated with chronological age, pubertal stage and pre-exercise sT levels (p < 0.01), whereas the ΔsT and ΔsT% of increase were negatively correlated with chronological age and resting sT levels (p < 0.05 and p < 0.01). In the present study, we observed the presence of high inter-individual variability of different biological parameters (anthropometry, pubertal stage, hormones, etc.) within athletes selected by chronological age in the same class and the presence of significant correlations between chronological age, puberty and the steroid hormone responses to physical exercise. Our data suggest the need for different criteria in exercise prescription and selection of young athletes and in the evaluation of stress reactivity at puberty.
Key words
Testosterone - cortisol - puberty - soccer
References
-
1
Aardal-Eriksson E, Karlberg B E, Holm A C.
Salivary cortisol - an alternative to serum cortisol determinations in dynamic function tests.
Clin Chem Lab Med.
1998;
36
215-222
-
2
Aubert J, Segura J.
Salivary cortisol as a marker of competition related stress.
Sci Sports.
1995;
10
149-154
-
3 Beunen G, Malina R. Growth and biological maturation: relevance to athletic performance. Bar-Or O The Child and Adolescent Athlete. Oxford; Blackwell Science UK 1996: 3-24
-
4
Boisseau N, Delamarche P.
Metabolic and hormonal responses to exercise in children and adolescents.
Sports Med.
2000;
30
405-422
-
5
Booth A, Shelley G, Mazur A, Tharp G, Kittok R.
Testosterone and winning and losing in human competition.
Horm Behav.
1989;
23
556-571
-
6
Bunc V, Psotta R.
Physiological profile of very young soccer players.
J Sports Med Phys Fitness.
2001;
41
337-341
-
7
Campbell B, O'Rourke M T, Lipson S F.
Salivary testosterone and body composition in Arial males.
Am J Human Biol.
2003;
15
697-708
-
8
Chicharro J L, Lucía A, Pérez M, Vaquero A F, Ureña R.
Saliva composition and exercise.
Sports Med.
1998;
26
17-27
-
9 Cumming D C. Hormones and athletic performance. Felig P, Baxter JD, Frohman LA Endocrinology and Metabolism. New York; Mc Graw-Hill 1995: 1837-1886
-
10
Dabbs Jr J M, Campbell B C, Gladue B A, Midgley A R, Navarro M A, Read G F, Susman E J, Swinkels L M, Worthman C M.
Reliability of salivary testosterone measurements: a multicenter evaluation.
Clin Chem.
1995;
41
1581-1584
-
11
Del Corral P, Mahon A D, Duncan G E, Howe C A, Craig B W.
The effect of exercise on serum and salivary cortisol in male children.
Med Sci Sports Exerc.
1994;
26
1297-1301
-
12
Di Luigi L, Guidetti L, Pigozzi F, Baldari C, Casini A, Nordio M, Romanelli F.
Acute amino acids supplementation enhances pituitary responsiveness in athletes.
Med Sci Sports Exerc.
1999;
31
1748-1754
-
13
Eisenmann J C, Malina R M.
Age- and sex-associated variation in neuromuscular capacities of adolescent distance runners.
J Sports Sci.
2003;
21
551-557
-
14
Elloumi M, Maso F, Michaux O, Robert A, Lac G.
Behaviour of saliva cortisol (C), testosterone (T) and the T/C ratio during a rugby match and during the post-competition recovery days.
Eur J Appl Physiol.
2003;
90
23-28
-
15
Fahey T D, Del Valle-Zuris A, Oehlsen G, Trieb M, Seymour J.
Pubertal stage differences in hormonal and haematological responses to maximal exercise in males.
J Appl Physiol.
1979;
46
823-827
-
16
Filaire E, Bernain X, Sagnol M, Lac G.
Preliminary results on mood state, salivary testosterone : cortisol ratio and team performance in a professional soccer team.
Eur J Appl Physiol.
2001;
86
179-184
-
17
Gomez F, Manalo S, Dallman M F.
Androgen-sensitive changes in regulation of restraint-induced adrenocorticotropin secretion between early and late puberty in male rats.
Endocrinology.
2004;
145
59-70
-
18
Gonzales-Bono E, Salvador A, Serrano M A, Ricarte J.
Testosterone, cortisol and mood in a sports team competition.
Horm Behav.
1999;
35
55-62
-
19
Granger D A, Shirtcliff E A, Zahn-Waxler C, Usher B, Klimes-Dougan B, Hastings P.
Salivary testosterone diurnal variation and psychopathology in adolescent males and females: individual differences and developmental effects.
Dev Psychopathol.
2003;
15
431-449
-
20
Groschl M, Rauh M, Dorr H G.
Circadian rhythm of salivary cortisol, 17 alpha-hydroxyprogesterone, and progesterone in healthy children.
Clin Chem.
2003;
49
1688-1691
-
21
Gurd B, Klentrou P.
Physical and pubertal development in young male gymnasts.
J Appl Physiol.
2003;
95
1011-1015
-
22
Hackney A C.
Endurance training and testosterone levels.
Sports Med.
1989;
8
117-127
-
23
Helsen W F, Starkes J L, Van Winckel J V.
Effect of a change in selection year on success in male soccer players.
Am J Human Biol.
2000;
12
729-735
-
24 Inbar O. Development of anaerobic power and local muscular endurance. Bar-Or O The Child and Adolescent Athlete. Oxford; Blackwell Science UK 1996: 42-53
-
25
Kiess W, Meidert A, Dressendörfer R A, Schriever K, Kessler U, König A, Schwarz H P, Strasburger C J.
Salivary cortisol levels throughout childhood and adolescence: relation with age, pubertal stage, and weight.
Pediatr Res.
1995;
37
502-506
-
26
Kraemer W J, Loebel C C, Volek J S, Ratamess N A, Newton R U, Wickham R B, Gotshalk L A, Duncan N D, Mazzetti S A, Gomez A L, Rubin M R, Nindl B C, Häkkinen K.
The effect of heavy resistance exercise on the circadian rhythm of salivary testosterone in men.
Eur J Appl Physiol.
2001;
84
13-18
-
27
Lac G, Pantelidis D, Robert A.
Salivary cortisol response to a 30 min submaximal test adjusted to a constant heart rate.
J Sports Med Phys Fitness.
1997;
37
56-60
-
28
Leatt P, Shepard R J, Plyley M J.
Specific muscular development in under-18 soccer players.
J Sports Sci.
1987;
5
165-175
-
29
Malina R M, Eisenmann J C, Cumming S P, Ribeiro B, Aroso J.
Maturity-associated variation in the growth and functional capacities of youth football (soccer) players 13 - 15 years.
Eur J Appl Physiol.
2004;
91
555-562
-
30
McMurray R G, Harrel J S, Bangdiwala S I, Hu J.
Tracking of physical activity and aerobic power from childhood through adolescence.
Med Sci Sports Exerc.
2003;
35
1914-1922
-
31
Naughton G, Farpour-Lambert N J, Carlson J, Bradney M, Van Praagh E.
Physiological issues surrounding the performance of adolescent athletes.
Sports Med.
2000;
30
309-325
-
32
Netherton C, Goodyer I, Tamplin A, Herbert J.
Salivary cortisol and dehydroepiandrosterone in relation to puberty and gender.
Psychoneuroendocrinology.
2004;
29
125-140
-
33
Nicolson N, Storms C, Ponds R, Sulon J.
Salivary cortisol levels and stress reactivity in human aging.
J Gerontol.
1997;
52
68-75
-
34
O'Connor P J, Corrigan D L.
Influence of short term cycling on salivary cortisol levels.
Med Sci Sports Exerc.
1987;
19
224-228
-
35
Ohzeki T, Manella B, Gubelin-De Campo C, Zachmann M.
Salivary testosterone concentrations in prepubertal and pubertal males: comparison with total and free plasma testosterone.
Horm Res.
1991;
36
235-237
-
36
Ostatníková D, Pastor K, Putz Z, Dohnányiová M, Mat'ašeje A, Hampl R.
Salivary testosterone in preadolescent children.
BMC Pediatr.
2002;
2
1-5
-
37
Passelergue P, Lac G.
Salivary cortisol, testosterone and T/C variations during a wrestling competition and during the post-competitive recovery period.
Int J Sports Med.
1999;
20
109-113
-
38
Passelergue P, Robert A, Lac G.
Salivary cortisol and testosterone variations during an official and a simulated weight-lifting competition. Int.
J Sports Med.
1995;
16
298-303
-
39
Port K.
Serum and saliva cortisol responses and blood lactate accumulation during incremental exercise testing.
Int J Sports Med.
1991;
12
490-494
-
40
Raivio T, Dunkel L, Wickman S, Jänne O A.
Serum androgen bioactivity in adolescence: a longitudinal study of boys with constitutional delay of puberty.
J Clin Endocrinol Metab.
2004;
89
1188-1192
-
41
Rilling J K, Worthman C M, Campbell B C, Stallings J F, Mbizva M.
Ratios of plasma and salivary testosterone throughout puberty: production vs. bioavailability.
Steroids.
1996;
61
374-378
-
42
Rosmond R, Björntorp P.
New targets for the clinical assessment of salivary cortisol secretion.
J Endocrinol Invest.
2001;
24
639-641
-
43
Rosmond R, Dallman M F, Björntorp P.
Stress-related cortisol secretion in men: relationships with abdominal obesity and endocrine, metabolic and hemodynamic abnormalities.
J Clin Endocrinol Metab.
1998;
83
1853-1859
-
44
Shoal G D, Giancola P R, Kirillova G P.
Salivary cortisol, personality, and aggressive behaviour in adolescent boys: a 5-year longitudinal study.
J Am Acad Child Adolesc Psychiatry.
2003;
42
1101-1107
-
45
Sills I N, Cerny F J.
Responses to continuous and intermittent exercise in healthy and insulin dependent diabetic children.
Med Sci Sports Exerc.
1983;
15
450-454
-
46
Slaughter M H, Lohman T G, Boileau R A, Horswill C A, Stillman R J, Van Loan M D, Bemben D A.
Skinfold equations for estimation of body fatness in children and youth.
Hum Biol.
1988;
60
709-723
-
47
Smilios I, Pilianidis T, Karamouzis M, Tokmakidis S P.
Hormonal responses after various resistance exercise protocols.
Med Sci Sports Exerc.
2003;
35
644-654
-
48
Stubbe J H, Boomsma D I, De Geus E J.
Sports participation during adolescence: a shift from environmental to genetic factors.
Med Sci Sports Exerc.
2005;
37
563-570
-
49
Stupnicki R, Obminski Z.
Glucocorticoids response to exercise as measured by serum and salivary cortisol.
Eur J Appl Physiol.
1992;
65
546-549
-
50
Tanner J M, Whitehouse R H.
Clinical longitudinal standards for height, weight, height velocity, weight velocity and stages of puberty.
Arch Dis Child.
1976;
51
170-179
-
51
Tornhage C J.
Reference values for morning salivary cortisol concentrations in healthy school-aged children.
J Pediatr Endocrinol Metab.
2002;
15
197-204
-
52
Tsolakis C, Vagenas G, Dessypris A.
Growth and anabolic hormones, leptin, and neuromuscular performance in moderately trained prepubescent athletes and untrained boys.
J Strength Cond Res.
2003;
17
40-46
-
53 Viru A, Viru M, Kuusler K. Exercise-induced hormonal response in boys in relation to sexual maturation. XXth International Symposium of the European Group of Pediatric Work Physiology. 1999: 135
-
54
Weise M, Eisenhofer G, Merke D P.
Pubertal and gender-related changes in the sympathoadrenal system in healthy children.
J Clin Endocrinol Metab.
2002;
87
5038-5043
-
55
Winter J S.
The metabolic response to exercise and exhaustion in normal and growth-hormone-deficient children.
Can J Physiol Pharmacol.
1974;
52
575-582
-
56
Zachman M, Prader A, Kind H P, Hafliger H, Budliger H.
Testicular volume during adolescence.
Helv Paediatr Acta.
1974;
29
61-72
-
57
Zitzmann M, Nieschlag E.
Testosterone levels in healthy men and the relation to behavioural and physical characteristics: facts and constructs.
Eur J Endocrinol.
2001;
144
183-197
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