The Effect of Genotype and Cultivation Method on the Total Salicylate Yield of Dark-Leaved Willows (Salix myrsinifolia)

Abstract

Ten clones of dark-leaved willows (Salix myrsinifolia Salisb.) were grown for two years on two different sites, Luikonlahti and Punkaharju, using various combinations of soil cultivation, fertilization and mulch treatments, with the objective of comparing the effect of different cultivation methods on the growth and total salicylate yield. In cultivated soil, the total salicylate yield ranged from 20 to 220 kg/ha, depending on the clone and cultivation method. The use of black polythene mulch and the addition of mineral fertilizer reduced the total salicylate concentrations of several clones. On the other hand, polythene mulch noticeably increased the hectare yield of total salicylates by promoting biomass accumulation.

Abbreviations

LE:Luikonlahti experiment

PE:Punkaharju experiment

Key words

Salix myrsinifolia - total salicylates - cultivation - fertilization - mulch treatment

References

• 1 Julkunen-Tiitto R. Phenolic constituents of Salix: A chemotaxonomic survey of further Finnish species.  Phytochemistry. 1989;  28 2115-25
• 2 Schmid B, Lüdtke R, Selbmann H K, Kötter I, Tschirdemwahn B, Schaffner W. et al . Efficacy and tolerability of a standardized willow bark extract in patients with osteoarthritis: randomised placebo-controlled, double blind clinical trial.  Phytother Res. 2001;  15 344-50
• 3 Julkunen-Tiitto R, Meier B. Variation in growth and secondary phenolics among field-cultivated clones of Salix myrsinifolia .  Planta Med. 1992;  58 77-80
• 4 Davies R. Sheet mulching as an aid to broadleaved tree establishment I. The effectiveness of various synthetic sheets compared.  Forestry. 1988;  61 89-105
• 5 Adegbidi H, Briggs R, Volk T, White E, Abrahamson L. Effect of organic amendments and slow-release nitrogen fertilizer on willow biomass production and soil chemical characteristics.  Biomass Bioenerg. 2003;  25 389-98
• 6 Hakulinen J, Julkunen-Tiitto R, Tahvanainen J. Does nitrogen fertilization have an impact on the trade-off between willow growth and defensive secondary metabolism?.  Trees. 1995;  9 235-40
• 7 Orians C, Lower S, Fritz R, Roche B. The effects of plant genetic variation and soil nutrients on secondary chemistry and growth in a shrubby willow, Salix sericea: patterns and constraints on the evolution of resistance traits.  Biochem Syst Ecol. 2003;  31 223-47
• 8 Vuorinen J, Mäkitie O. The method of soil testing in use in Finland.  Agrogeol Publ. 1955;  63 1-44
• 9 Cochran W, Cox G ( editors). Experimental Designs. 2nd edn New York; John Wiley & Sons 1957: pp 293-316
• 10 Meier B, Sticher O, Bettschart A. Weidenrinden - Die Qualität.  Deutsche Apotheker Zeitung. 1985;  7 341-7
• 11 Houle G, Babeux B. Fertilizing and mulching influence on the performance of four native woody species suitable for revegetation in subarctic Quebec.  Can J For Res. 1994;  24 2342-9
• 12 Hytönen J, Kaunisto S. Effect of fertilization on the biomass production of coppiced mixed birch and willow stands on a cut-away peatland.  Biomass Bioenerg. 1999;  17 455-69
• 13 Green D, Kruger E, Stanosz G. Effects of polyethylene mulch in a short-rotation, poplar plantation vary with weed-control strategies, site quality and clone.  For Ecol Manag. 2003;  173 251-60
• 14 Labrecque M, Teoderescu T, Babeux P, Cogliastro A, Daigle S. Impact of herbaceous competition and drainage conditions on the early productivity of willows under short-rotation intensive culture.  Can J For Res. 1994;  24 493-501
• 15 Ruuhola T, Julkunen-Tiitto R. Trade-off between the synthesis of salicylates and the growth of micropropogated Salix pentandra plants.  J Chem Ecol. 2003;  29 1565-88
• 16 Adegbidi H, Volk T, White E, Abrahamson L, Briggs R, Bickelhaupt D. Biomass and nutrient removal by willow clones in experimental bioenergy plantations in New York State.  Biomass Bioenerg. 2001;  20 399-411