Geographic Information System Method for Assessing Chemo-Diversity in Medicinal Plants

Abstract

The spatial distribution of wild germplasm of Podophyllum peltatum L. (American mayapple) has been analyzed using the Geographic Information System (GIS) with the objective to develop a method and a database for evaluation of biotic and abiotic factors influencing drug yield, and to map elite genotypes for propagation and improvement. The field assessment followed a standard procedure including geographical coordinates of each accession, leaf biomass randomly harvested, identification of associate species, collection of herbarium specimen, soil sample and digital pictures of the site. By overlaying morphological and chemical data with geomorphic information, a thematic map was created locating the podophyllotoxin-rich accessions and the uniqueness of each site was recorded for post-collection analysis. This work has enabled the establishment of a database of P. peltatum germplasm in Mississippi with drug yield linked to spatial locations for rational utilization of our natural resources. While this method integrates information of well-characterized diverse in situ P. peltatum germplasm, it might become a strategy for curators to reduce cost for establishing and maintaining ex situ collections since the genetic material is geo-referenced.

Abbreviations

GIS:geographical information system

GPS:global positioning system

DGPS:differential global positioning system

DEM:digital elevation model

Podophyllum - germplasm acquisition - geographical information system

References

• 1 Brown A HD, Brubaker C L, Grace J P. Regeneration of germplasm samples: wild versus cultivated plant species.  Crop Sci. 1997;  37 7-13
• 2 Greene S L, Hart R A, Afonin A. Using geographic information to acquire wild crop germplasm for ex situ collection: II post collection analysis.  Crop Sci. 1999;  29 843-9
• 3 Canel C, Dayan F E, Ganzera M, Rimando A, Burandt J r. C, Khan I et al. High yield of podophyllotoxin from leaves of Podophyllum peltatum by in situ conversion of podophylotoxin 4-O-β- D-glucopyranoside.  Planta Med. 2001;  67 97-9
• 4 Moraes R M, Burandt J r. C, Ganzera M, Li X, Khan I, Canel C.  The American mayapple revisited-Podophyllum peltatum - still a potential cash crop? Econ Bot. 2000;  54 471-76
• 5 Moraes R M, Bedir E, Barrett H, Burandt Jr C, Canel C, Khan I. Evaluation of Podophyllum peltatum L. accessions for podophyllotoxin production.  Planta Med. 2002;  68 341-4
• 6 Cushman K E, Maqbool M, Bedir E, Lata H, Khan I A, Moraes R M. Growth and lignan content of American mayapple under increasing levels of shade.  Hort Sci. 2005;  40 60-3
• 7 Crouse K K. Soil testing and plant analysis in Mississippi. Procedure used by the MSU-ES testing laboratory. Mississippi State University 2001: pp 14-30
• 8 NOAA . National Oceanic and Atmospheric Administration at the National Climatic Data Center web site 2004 at: http://lwf.ncdc.noaa.gov/oa/ncdc.html. 
• 9 MARIS . Mississippi Automated Resources Information System Technical Center web site 2004 at: http://www.maris.state.ms.us. 
• 10 Eason A, Tim U S, Wang X. Integrated modeling environment for statewide assessment of groundwater vulnerability from pesticide use in agriculture.  Pest Manag Sci. 2004;  60 739-45
• 11 Nemenyi M, Mesterhazi P A, Pecze Z, Stepan Z. The role of GIS and GPS in precision farming.  Comput Electr Agric. 2003;  40 45-55
• 12 Pozdnyakova L, Oudemans P V, Hughes M G, Gimenez D. Estimation of spatial and spectral properties of Phytophthora root rot and its effects on cranberry yield.  Comput Electr Agric. 2002;  37 57-70
• 13 Moraes R M, de Andrade Z, Bedir E, Dayan F, Lata H, Khan I. et al . Arbuscular mycorrhiza improves acclimatization and increases lignan content of micropropagated mayapple (Podophyllum peltatum L.)  Plant Sci. 2004;  166 23-9