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DOI: 10.1055/s-2006-931569
© Georg Thieme Verlag KG Stuttgart · New York
Antimalarial Activity of Biflavonoids from Ochna integerrima
Prof. Dr. Haruki Yamada
Kitasato Institute for Life Sciences
Kitasato University
5-9-1 Shirokane
Minato-ku
Tokyo 108-8641
Japan
Telefon: +81-3-3444-6164
Fax: +81-3-3445-1351
eMail: yamada@lisci.kitasato-u.ac.jp
Publikationsverlauf
Received: December 1, 2005
Accepted: January 17, 2006
Publikationsdatum:
29. Mai 2006 (online)
Abstract
During the screening of antimalarial substances, the 80 % EtOH extract from the outer bark of Ochna integerrima Merr. (Ochnaceae) was shown to have a good anti-malarial activity (IC50 value: 6.5 μg/mL) whereas extracts from the inner barks of O. integerrima showed no antimalarial activity. Biflavanone (1), which had not been found previously from a natural plant source, was isolated as a potent antimalarial active ingredient (IC50 value: 80 ng/mL) from the extract of the outer barks. The stereoisomer of 1 ( = compound 2) was also isolated from this plant; however, its activity was significantly lower than that of 1.
#Introduction
Malaria is the major parasitic infection in many tropical and subtropical regions, leading to more than one million deaths (principally among African children) out of 400 million cases each year [1] and to major consequent impacts on economic productivity and livelihood [2]. The incidence of malaria is now increasing because of the appearance of multi-drug resistant Plasmodium falcipalum, therefore new and more effective antimalarial drugs are urgently required.
Ochna integerrima Merr. (Ochnaceae) is a tree that is widely distributed in Thailand [3], and the bark of O. integerrima Merr. has been used for digestive disorders as a folk medicine in Thailand [4]. In phytochemical studies on O. integerrima Merr, many flavonoids have been isolated [5], [6], [7]. During a screening for antimalarial active sources among Thai plants, it was found that the 80 % EtOH extract from the outer bark of O. integerrima Merr. showed significant anti-malarial activity. The active ingredients in the outer bark were clarified in the present study.
#Materials and Methods
#Apparatus
Optical rotations were measured on a JASCO polarimeter. 1H- and 13C- NMR spectra were determined on a Varian Mercury-300. Mass spectra (MS) were obtained on JEOL MXA-AM505HA and JMS-700 MStation spectrometers. Chromatographic separations were carried out by column chromatography on Wakogel C-200 (75 - 150 μm, Wako; Osaka, Japan). Preparative reverse phase HPLC was carried out on PEGASIL (250 × 20 mm i. d., Senshu Co. Ltd.; Tokyo, Japan).
#Analytical methods
Analytical HPLC experiments were performed on an Agilent 1100 series HPLC instrument (Agilent Technologies Japan, Ltd.; Tokyo, Japan) equipped with a column of PEGASIL (250 × 4.6 mm i. d., Senshu Co. Ltd.; Tokyo, Japan). The solvent system used was a linear gradient of acetonitrile from 30 % to 60 % during 30 minutes in 10 mM phosphoric acid. Flow rate was 1 mL/min. Injection volume was 10 μL for MeOH solution of all the samples. Concentrations of the samples were 10 mg/mL for extracts and 1 mg/mL for the isolated compounds.
#Plant material
The outer and inner barks of O. integerrima Merr. were collected from Ubon Ratchathani in Thailand. The plants were identified by Associate Professor Wongsatit Chuakul, Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University. Voucher specimens are kept at the Herbarium of Faculty of Pharmacy, Mahidol University.
#Extraction and isolation
The outer barks of O. integerrima (485 g) were extracted with 80 % EtOH at room temperature for 3 days, and the extract was evaporated under vacuum to yield 150 g of the crude EtOH extract. The EtOH extract was chromatographed on silica gel (500 g) and eluted with CHCl3/MeOH (10 : 1, 1 : 1, 0 : 1; 2 L each) to give three fractions (Fr. 1 - 3). Fr. 1 (50 g, CHCl3/MeOH, 10 : 1) was further fractionated by silica gel column chromatography (1000 g) using CHCl3/MeOH containing 0.05 % TFA [50 : 1 (3 L), 20 : 1 (2 L), 10 : 1 (2 L), 5 : 1 (4 L), 1 : 1 (2 L)] to give eight fractions (Fr. 1/1 - 1/8). Fr. 1/4 (0.5 g of 16.5 g, CHCl3/MeOH, 5 : 1) was separated by a reverse phase HPLC (40 % CH3CN containing 0.05 % TFA, flow rate, 8.0 mL/min; UV detector, 254 nm) to give the active fraction (Fr. 1/4 - 3, 266.8 mg, t R = 19.2 min). Fr. 1/4 - 3 (133.4 mg) was further purified by the same reverse phase HPLC (solvent A, 40 % MeOH containing 0.05 % TFA; B, MeOH; A/B, 7 : 1 → 6 : 2 for 40 minutes; flow rate 8.0 mL/min; UV detector; 254 nm) to give 1 (97.9 mg, t R = 38.0 min) and 2 (7.3 mg, t R = 41.8 min).
Compound 1 : brown oil; [α]D 25: + 6.2 ° (c 1.00, MeOH); HR-FAB-MS: m/z = 511.1399 [M + H]+, (calcd. for C30H23O8 : 511.1393); LR-FAB-MS: m/z (rel. int.) = 511 (60), 255 (45); 1H-NMR (acetone-d 6, 300 MHz, 23 °C): δ = 6.29 (2H, d, J = 2.0 Hz, H-8/H-8′′), 6.59 (2H, dd, J = 8.5, 2.0 Hz, H-6/H-6′′), 6.88 (4H, d, J = 8.5 Hz, H-3′/H-3′′′ and H-5′/H-5′′′), 7.14 (4H, d, J = 8.5 Hz, H-2′/H-2′′′ and H-6′/H-6′′′), 7.78 (2H, d, J = 8.5 Hz, H-5/H-5′′); 13C-NMR (acetone-d 6, 75 MHz, 23 °C): δ = 49.8 (C-3/C-3′′), 82.5 (C-2/C-2′′), 103.4 (C-8/C-8′′), 111.5 (C-6/C-6′′), 114.4 (C-4a/C-4a′′), 116.2 (C-3′/C-3′′′ and C-5′/C-5′′′), 129.1 (C-1′/C-1′′′), 130.0 (C-5/C-5′′), 130.6 (C-2′/C-2′′′ and C-6′/C-6′′′), 159.0 (C-4′/C-4′′′), 164.2 (C-8a/C-8a′′), 165.4 (C-7/C-7′′), 190.7 (C-4/C-4′′).
Compound 2: brown oil; [α]D 25: + 83.2 ° (c 0.25, MeOH); HR-FAB-MS: m/z = 533.1209 [M + H]+, (calcd. for C30H22O8Na: 533.1212); 1H-NMR (acetone-d 6, 300 MHz, 23 °C): δ = 2.73 (2H, d, J = 12.0 Hz, H-3/H-3′′), 5.98 (2H, d, J = 12.0 Hz, H-2/H-2′′), 6.33 (2H, d, J = 1.5 Hz, H-8/H-8′′), 6.58 (2H, dd, J = 8.5, 1.5 Hz, H-6/H-6′′), 6.86 (4H, d, J = 8.0 Hz, H-3′/H-3′′′ and H-5′/H-5′′′), 7.02 (4H, d, J = 8.0 Hz, H-2′/H-2′′′ and H-6′/H-6′′′), 7.75 (2H, d, J = 8.5 Hz, H-5/H-5′′); 13C-NMR (acetone-d 6, 75 MHz, 23 °C): δ = 51.8 (C-3/C-3′′), 84.7 (C-2/C-2′′), 103.4 (C-8/C-8′′), 111.3 (C-6/C-6′′), 115.2 (C-4a/C-4a′′), 116.2 (C-3′/C-3′′′ and C-5′/C-5′′′), 129.1 (C-1′/C-1′′′), 129.8 (C-5/C-5′′), 130.1 (C-2′/C-2′′′ and C-6′/C-6′′′), 159.0 (C-4′/C-4′′′), 164.2 (C-8a/C-8a′′), 165.3 (C-7/C-7′′), 191.6 (C-4/C-4′′).
Sikokianins B and C: Both were isolated from Wikstroemia indica as reported previously [11].
#Antimalarial activity and cytotoxicity
The assays were performed as described previously [13]. Anti-malarial assays were conducted using the multidrug-resistant (K1) and drug-sensitive strains (FCR3) of Plasmodium falciparum (gift from Prof. K. Kita, University of Tokyo, Tokyo, Japan). Chloroquine (Sigma-Aldrich Japan K.K.; Tokyo, Japan) and artemisinin (Sigma-Aldrich Japan K.K.; Tokyo, Japan) were used as positive controls. Cytotoxicity was assayed against the human diploid embryonic cell line MRC-5 as described previously [13].
#Results and Discussion
The 80 % EtOH extract of the outer barks of O. integerrima showed significant antimalarial activity (IC50 value: 6.5 μg/mL against the multidrug-resistant strain of P. falcipalum and 4.5 μg/mL against the sensitive one, respectively). However, the extract from the inner barks of O. integerrima was not active (Table [1]). According to bioassay-guided purification, a biflavanone 1 was isolated as the major active compound (IC50 value: 80 ng/mL). The stereoisomer of 1, compound 2 (Fig. [1]), was also found in the extract as the other active ingredient, but 2 showed significantly weaker activity than 1 (Table [1]).
The structure of 1 was analyzed by 1D and 2D NMR, MS and optical rotation. The HR-FAB-MS of 1 gave [M + H]+ at m/z = 511.1393 and LR-FAB-MS gave the fragment ion at m/z = 255 in addition to [M + H]+. The presence of seven phenyl protons and twelve phenyl carbons was confirmed with a 1D-NMR spectrum, and a keto group (δ = 190.7) was also detected in the 13C-NMR spectrum. In addition, 1 was also analyzed by HMQC and HMBC experiments, and the obtained data for 1 were very similar to the reported data for the meso form of 3,3′′-di(7,4′-dihydroxyflavanone-3-yl) which had been obtained only as the biotransformation product [8], [9]. Although the signals of H-2, H-2′′, H-3 and H-3′′ almost could not be observed due to the fixed conformation at 23 °C, these appeared as doublets (δH = 4.86, 2H, d, J = 12.0 Hz, H-2/H-2′′ and 3.55, 2H, d, J = 12.0 Hz, H-3/H-3′′) on NMR analysis at 80 °C in DMSO-d 6 in a similar manner as reported previously [9], suggesting that H-2, H-2′′, H-3 and H-3′′ were each located at axial sites. Compound 1 also gave a very small optical rotation, and from these observations 1 was identified to be the meso form of 3,3′′-di(7,4′-dihydroxyflavanone-3-yl) (Fig. [1]). Compound 2 gave similar spectra upon 1H-NMR, 13C-NMR and MS analyses as the other biotransformation product, the diastereomer of 1 [9]. Although this biotransformation product of 1 has been reported as the racemate, which showed no optical rotation [9], 2 showed significant dextrorotation (+ 83.2° in MeOH at 25 °C). These findings suggested that 2 might be a diastereomer of 1, but the absolute stereostructure of 2 could not be determined in the present study. Compound 1 and the racemate of 2 had been obtained only as biotransformation products from chalcones with peroxidases of cultured plant cells [8], [9], [10]. The present paper is the first report on the isolation of compounds 1 and 2 directly from natural plant extracts. When the 80 % extracts from both the inner and outer barks of O. integerrima were compared by HPLC, compounds 1 and 2 could not be detected in the extract from the inner barks, therefore explaining why the extracts of the inner barks were not active (Fig. [2]).
Compounds 1 and 2 were evaluated in vitro for antimalarial activity (Table [1]). The antimalarial activity of compound 1 against the multidrug-resistant strain (K1) was three times stronger than against the sensitive strain (FCR3), and the selectivity (IC50 value against MRC-5/IC50 value against K1) was 375. However, the antimalarial activity of compound 2 was 65 and 17 times weaker than those of 1 for the K1 and FCR3 strains, respectively, although similar cytotoxic activities against MRC-5 were observed for both compounds 1 and 2.
The antimalarial biflavanones, sikokianins B and C, have been isolated from Wikstroemia indica [11], and sikokianins B and C both showed ten times weaker activity than compound 1, assuming that the stereochemistry of the C3/C3′′ coupling bond and methoxy substitution might affect the degree of activity. The monomer of compound 1 is liquiritigenin which has no antiplasmodial activity, while a chalcone (could be related to 1/2), namely isoliquiritigenin, has weaker in vitro antimalarial activity than the present biflavanone (1) [12]. Finally, biflavanone 1 could be a promising compound for investigation of in vivo antimalarial activity in animal models.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 1 Antimalarial biflavanones from Ochna integerrima Merr.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 2 HPLC profiles at 254 nm of (a) the extract of inner bark of Ochna integerrima Merr.; (b) the extract of outer bark of Ochna integerrima Merr.; (c) compound 1; (d) compound 2.
Extract/Fraction/Compound | K1 [μg/mL] | FCR3 [μg/mL] | MRC-5 [μg/mL] | Selectivityc |
80 % EtOH extract (outer barks) | 6.5 | 4.5 | 4.5 | 0.7 |
80 % EtOH extract (inner barks) | > 12.5 | N.T.b | N.T.b | |
Fraction 1 | 5.0 | 4.5 | 4.5 | 0.9 |
Fraction 2 | 40.0 | 40.0 | 40.0 | 1.0 |
Fraction 3 | 50.0 | 45.0 | 45.0 | 0.9 |
1 | 0.08 | 0.26 | 30.0 | 375 |
2 | 5.2 | 4.5 | 27.0 | 5.2 |
Sikokianin B | 0.54 | 0.54 | 22.5 | 41.7 |
Sikokianin C | 0.56 | 0.34 | 11.2 | 20.0 |
Artemisinina | 0.007 | 0.005 | N.T.b | |
Chloroquinea | 0.11 | 0.009 | N.T.b | |
a Positive control. | ||||
b N.T. = not tested. | ||||
c IC50 on MRC-5/IC50 on K1. |
Acknowledgements
This work was supported, in part, by funds from the UNICEF/UNDP/World Bank/WHO special Program for Research and Training in Tropical Disease (grants ID A10124), the 21st century COE Program, Ministry of Education, Culture, Sports, Science and Technology (MEXT), and JSPS/NRCT Core University Program for Natural Products in the category of pharmaceutical sciences. The authors appreciate Ms. A. Nakagawa and Ms. C. Sakabe for mass measurements and Ms. N. Sato for NMR measurements.
#References
- 1 Greenwood B, Mutabingwa T. Malaria in 2002. Nature. 2002; 415 670-2
- 2 Sachs J, Malaney P. The economic and social burden of malaria. Nature. 2002; 415 680-5
- 3 Smitinand T, Larsen K. Flora of Thailand. Vol. 2 Bangkok; Tistr Press 1970: p 24-6
- 4 Perry L M. Medicinal plants of East and Southeast Asia. Massachusetts; MIT Press 1980: p 289-90
- 5 Likhitwitayawuid K, Rungserichai R, Ruangrungsi N, Phadungcharoen T. Flavonoids from Ochna integerrima . Phytochemistry. 2001; 56 353-7
- 6 Kaewamatawong R, Likhitwitayawuid K, Ruangrungsi N, Takayama H, Kitajima M, Aimi N. Novel biflavonoids from the stem bark of Ochna integerrima . J Nat Prod. 2002; 65 1027-9
- 7 Likhitwitayawuid K, Kaewamatawong R, Ruangrungsi N. Mono- and biflavonoids of Ochna integerrima . Biochem Syst Ecol. 2005; 33 527-36
- 8 Botta B, Vinciguerra V, DeRosa M C, Scurria R, Carbonetti A, Ferrari F. et al . Studies in cell suspension cultures of Cassia didymobotrya. Part III. The biotransformation of chalcones to flavones and biflavanones. Heterocycles. 1989; 29 2175-83
- 9 Vitali A, Botta B, Delle Monache G, Zappitelli S, Ricciardi P, Melino S. et al . Purification and partial characterization of a peroxidase from plant cell cultures of Cassia didymobotrya and biotransformation studies. Biochem J. 1998; 331 513-9
- 10 Botta B, Ricciardi P, Vitali A, Vinciguerra V, Garcia C, Delle Monache G. Peroxidase from cell cultures of Cassia didymobotrya: a review and comparison with horseradish peroxidase. Heterocycles. 1999; 50 757-66
- 11 Nunome S, Ishiyama A, Kobayashi M, Otoguro K, Kiyohara H, Yamada H. et al . In vitro antimalarial activity of biflavonoids from Wikstroemia indica . Planta Med. 2004; 70 76-8
- 12 Beldjoudi N, Mambu L, Labaied M, Grellier P, Ramanitrahasimbola D, Rasoanaivo P. et al . Flavonoids from Dalbergia louvelii and their antiplasmodial activity. J Nat Prod. 2003; 66 1447-50
- 13 Otoguro K, Kohana A, Manabe C, Ishiyama A, Ui H, Shiomi K. et al . Potent antimalarial activities of polyether antibiotic, X-206. J Antibiot. 2001; 54 658-63
Prof. Dr. Haruki Yamada
Kitasato Institute for Life Sciences
Kitasato University
5-9-1 Shirokane
Minato-ku
Tokyo 108-8641
Japan
Telefon: +81-3-3444-6164
Fax: +81-3-3445-1351
eMail: yamada@lisci.kitasato-u.ac.jp
References
- 1 Greenwood B, Mutabingwa T. Malaria in 2002. Nature. 2002; 415 670-2
- 2 Sachs J, Malaney P. The economic and social burden of malaria. Nature. 2002; 415 680-5
- 3 Smitinand T, Larsen K. Flora of Thailand. Vol. 2 Bangkok; Tistr Press 1970: p 24-6
- 4 Perry L M. Medicinal plants of East and Southeast Asia. Massachusetts; MIT Press 1980: p 289-90
- 5 Likhitwitayawuid K, Rungserichai R, Ruangrungsi N, Phadungcharoen T. Flavonoids from Ochna integerrima . Phytochemistry. 2001; 56 353-7
- 6 Kaewamatawong R, Likhitwitayawuid K, Ruangrungsi N, Takayama H, Kitajima M, Aimi N. Novel biflavonoids from the stem bark of Ochna integerrima . J Nat Prod. 2002; 65 1027-9
- 7 Likhitwitayawuid K, Kaewamatawong R, Ruangrungsi N. Mono- and biflavonoids of Ochna integerrima . Biochem Syst Ecol. 2005; 33 527-36
- 8 Botta B, Vinciguerra V, DeRosa M C, Scurria R, Carbonetti A, Ferrari F. et al . Studies in cell suspension cultures of Cassia didymobotrya. Part III. The biotransformation of chalcones to flavones and biflavanones. Heterocycles. 1989; 29 2175-83
- 9 Vitali A, Botta B, Delle Monache G, Zappitelli S, Ricciardi P, Melino S. et al . Purification and partial characterization of a peroxidase from plant cell cultures of Cassia didymobotrya and biotransformation studies. Biochem J. 1998; 331 513-9
- 10 Botta B, Ricciardi P, Vitali A, Vinciguerra V, Garcia C, Delle Monache G. Peroxidase from cell cultures of Cassia didymobotrya: a review and comparison with horseradish peroxidase. Heterocycles. 1999; 50 757-66
- 11 Nunome S, Ishiyama A, Kobayashi M, Otoguro K, Kiyohara H, Yamada H. et al . In vitro antimalarial activity of biflavonoids from Wikstroemia indica . Planta Med. 2004; 70 76-8
- 12 Beldjoudi N, Mambu L, Labaied M, Grellier P, Ramanitrahasimbola D, Rasoanaivo P. et al . Flavonoids from Dalbergia louvelii and their antiplasmodial activity. J Nat Prod. 2003; 66 1447-50
- 13 Otoguro K, Kohana A, Manabe C, Ishiyama A, Ui H, Shiomi K. et al . Potent antimalarial activities of polyether antibiotic, X-206. J Antibiot. 2001; 54 658-63
Prof. Dr. Haruki Yamada
Kitasato Institute for Life Sciences
Kitasato University
5-9-1 Shirokane
Minato-ku
Tokyo 108-8641
Japan
Telefon: +81-3-3444-6164
Fax: +81-3-3445-1351
eMail: yamada@lisci.kitasato-u.ac.jp
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 1 Antimalarial biflavanones from Ochna integerrima Merr.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 2 HPLC profiles at 254 nm of (a) the extract of inner bark of Ochna integerrima Merr.; (b) the extract of outer bark of Ochna integerrima Merr.; (c) compound 1; (d) compound 2.