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DOI: 10.1055/s-2006-931914
Profile of Serum S100β Levels during Maturation in Fetal and Neonatal Sheep
Publication History
Publication Date:
22 February 2006 (online)
ABSTRACT
Serum levels of the protein S-100β are dependent on three factors: rate of production, permeability of the blood-brain barrier, and rate of clearance. In the developing fetus and neonate all of these factors change at different rates. This study was performed to determine how serum S-100 levels varied during fetal and early postnatal life. Blood samples were obtained from 41 fetal and neonatal lambs. The blood was separated in a centrifuge and the serum drawn off and assayed for S-100β using a commercially available radioimunoassay kit. S-100β did not appear in the blood until halfway through pregnancy. Thereafter, levels steadily increased until 1 month after birth. Following this, S-100β levels decreased progressively until by 1 year of age, they had reached a plateau. S-100β levels change significantly with normal fetal and neonatal maturation. Valid interpretation of other data from subjects of similar developmental stage must take into consideration this physiological variation.
KEYWORDS
S-100 - brain maturation
REFERENCES
- 1 Gazzolo D, Vinesi P, Marinoni E et al.. S100B protein concentrations in cord blood: correlations with gestational age in term and preterm deliveries. Clin Chem. 2000; 46 998-1000
- 2 Distefano G, Curreri R, Betta P, Isaja M T, Romeo M G, Amato M. Serial protein S-100 serum levels in preterm babies with perinatal asphyxia and periventricular white matter lesions. Am J Perinatol. 2002; 19 317-322
- 3 Dwyer R, Fee J P, Moore J. Uptake of halothane and isoflurane by mother and baby during caesarean section. Br J Anaesth. 1995; 74 379-383
- 4 Rosen M A. Anesthesia for fetal procedures and surgery. Yonsei Med J. 2001; 42 669-680
- 5 Ikai A, Reimer R K, Ramamoorthy C et al.. Preliminary results of fetal cardiac bypass in nonhuman primates. J Thorac Cardiovasc Surg. 2005; 129 175-181
- 6 Westaby S, Johnsson P, Parry A J et al.. Serum S100 protein: a potential marker for cerebral events during cardiac surgery. Ann Thorac Surg. 1996; 61 88-92
- 7 Hidaka H, Endo T, Kawamoto S et al.. Purification and characterization of adipose tissue S-100b protein. J Biol Chem. 1983; 258 2705-2709
- 8 Stefansson K, Wollmann R L, Moore B W, Arnason B G. S-100 protein in human chondrocytes. Nature. 1982; 295 63-64
- 9 Haimoto H, Hosoda S, Kato K. Differential distribution of immunoreactive S100-a and S100-b proteins in normal nonnervous human tissues. Lab Invest. 1987; 57 489-498
- 10 Becker T, Gerke V, Kube E, Weber K. S100P, a novel Ca(2+)-binding protein from human placenta: cDNA cloning, recombinant protein expression and Ca2+ binding properties. Eur J Biochem. 1992; 207 541-547
-
11 Widdowson E M, Dickerson J W.
Composition of the body . In: Lentner C Geigy Scientific Tables. Vol. 1. Units of Measurement, Body Fluids, Composition of the Body, and nutrition. Basel, Switzerland; CIBA-GEIGY 1981: 217 - 12 Hyden H, Ronnback L. S100 on isolated neurons and glial cells from rat, rabbit and guinea pig during early postnatal development. Neurobiology. 1975; 5 291-302
- 13 Ghandour M S, Labourdette G, Vincendon G, Gombos G. A biochemical and immunohistological study of S100 protein in developing rat cerebellum. Dev Neurosci. 1981; 4 98-109
- 14 Zimmer D B, van Eldik L J. Secretion of S-100 from rat C6 glioma cells. Brain Res. 1987; 436 367-370
- 15 Kligman D, Marshak D R. Isolation and characterization of a neurite extension factor from bovine brain. Proc Natl Acad Sci U S A. 1985; 82 7136-7139
- 16 Ronnback L. Appearance and accumulation of the brain specific S100 protein in the developing nervous systems of rat, rabbit and guinea pig. Cytobios. 1976; 16 219-226
- 17 Patterson D SP, Sweasey D, Hebert C N. Changes occurring in the chemical composition of the central nervous system during foetal and post-natal development of the sheep. J Neurochem. 1971; 18 2027-2040
-
18 Dobbing J.
The later development of the brain and its vulnerability . In: Davis JA, Dobbing J Scientific Foundations of Paediatrics. London; Heinemann Medical 1974: 565-577 - 19 Adinolfi M, Haddad S A. Levels of plasma proteins in human and rat fetal CSF and the development of the blood-CSF barrier. Neuropediatrics. 1977; 8 345-353
- 20 Dziegielewska K M, Knott G W, Saunders N R. The nature and composition of the internal environment of the developing brain. Cell Mol Neurobiol. 2000; 20 41-56
- 21 Dziegielewska K M, Habgood M D, Mollgard K, Stagaard M, Saunders N R. Species-specific transfer of plasma albumin from blood into different cerebrospinal fluid compartments in the fetal sheep. J Physiol. 1991; 439 215-237
- 22 Mollgard K, Balslev Y, Lauritzen B, Saunders N R. Cell junctions and membrane specializations in the ventricular zone (germinal matrix) of the developing sheep brain: a CSF-brain barrier. J Neurocytol. 1987; 16 433-444
- 23 Marshak D R. S100b as a neurotropic factor. Prog Brain Res. 1990; 86 169-181
- 24 Hu J, Van Eldik L J. S100 beta induces apoptotic cell death in cultured astrocytes via a nitric oxide-dependent pathway. Biochim Biophys Acta. 1996; 1313 239-245
- 25 Kligman D. Isolation of a protein from bovine brain which promotes neurite extension from chick embryo cerebral cotex neurones in defined medium. Brain Res. 1982; 250 93-100
- 26 Kligman D, Marshak D R. Isolation and characterisation of a neurite extension factor from bovine brain. Proc Natl Acad Sci U S A. 1985; 82 7136-7139
- 27 Yang Q, Hamberger A, Wang S, Haglid K G. Appearance of neuronal S-100β during development of the rat brain. Dev Brain Res. 1996; 91 181-189
- 28 Zuckerman J E, Herschman H R, Levine L. Appearance of a brain specific antigen (the S-100 protein) during human fietal development. J Neurochem. 1970; 17 247-251
- 29 Tiu S C, Chan W Y, Heizmann C W, Schafer B W, Shu S Y, Yew D T. Differential expression of S100B and S100A6 in the human fetal and aged cerebral cortex. Dev Brain Res. 2000; 119 159-168
- 30 Ueda S, Saitoh Y, Koibuchi N, Ishizuya-Oka A. Local disturbance of neuronal migration in the S-100beta-retarded mutant mouse. Cell Tissue Res. 1997; 289 547-551
- 31 Makino E, Sakaguchi M, Iwatsuki K, Huh N H. Introduction of an N-terminal peptide of S100C/A11 into human cells induces apoptotic cell death. J Mol Med. 2004; 82 612-620
- 32 Yui S, Nakatani Y, Mikami M. Calprotectin (S100A8/S100A9), an inflammatory protein complex from neutrophils with a broad apoptosis-inducing activity. Biol Pharmacol Bull. 2003; 26 753-760
Andrew J ParryM.A. B.M.
Department of Pediatric Cardiac Surgery, Bristol Royal Hospital for Children
Upper Maudlin Street, Bristol BS2 8BJ, United Kingdom