Planta Med 2006; 72(13): 1193-1199
DOI: 10.1055/s-2006-947222
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Immunoactive Polysaccharide-Rich Fractions from Panax notoginseng

Ying Zhu1 , Filomena Pettolino1 , Shaio-lim Mau1 , Yu-Chang Shen2 , Chie-Fu Chen2 , Yuh-Chi Kuo3 , Antony Bacic1
  • 1Cooperative Research Centre for Bioproducts, School of Botany, University of Melbourne, Australia
  • 2National Research Institute of Chinese Medicine, Taipei, Taiwan, R.O.C.
  • 3Institute of Life Science, Fu-Jen University, Taipei, Taiwan, R.O.C.
Further Information

Publication History

Received: January 9, 2006

Accepted: July 2, 2006

Publication Date:
18 September 2006 (online)

Abstract

Panax notoginseng is a commonly used medicinal plant in south-western China. In a previous study, a sequential solubilisation of P. notoginseng high-molecular-weight (HMW) polymers using phenol-acetic acid-water, hot water, weak and strong alkali was performed to determine the structure of the component polysaccharides and proteins. The effects of these extracted HMW fractions on the human complement system, polymorphonuclear neutrophils (PMN) and peripheral blood mononuclear cells (PBMC) are reported here. Fr1MKOH, which was extracted with 1 M KOH, showed the strongest complement-fixing activity and priming of reactive oxygen species (ROS) production by PMNs, as well as a mitogenic effect. Fr1MKOH was further fractionated by anion-exchange chromatography followed by gel-permeation chromatography. 1MD3-G2, the fraction most strongly bound to the DEAE anion-exchange column with a molecular weight of 1140 kDa, showed the highest complement-fixing activity. It is composed of acidic polysaccharides [including glucuronoarabinoxylan (GAX), homogalacturonan (HGA), rhamnogalacturonan I (RG I)], neutral polysaccharides (4-galactan and arabinan), and some protein.

References

  • 1 Gao H. Immunostimulating polysaccharides from Chinese medicinal herbs: Panax notoginseng and Viola yedoensis . Dissertation, Los Angeles University of Southern California 1996
  • 2 Ma W G, Mizutani M, Malterud K E, Lu S L, Ducrey B, Tahara S. Saponins from the roots of Panax notoginseng .  Phytochemistry. 1999;  52 1133-9
  • 3 Zhu Y, Pettolino F, Mau S L, Bacic A. Characterization of cell wall polysaccharides from the medicinal plant Panax notoginseng .  Phytochemistry.. 2005;  66 1067-76
  • 4 Li X Y. Immunomodulating Chinese herbal medicines.  Mem Inst Oswaldo Cruz. 1991;  86 159-64
  • 5 Ohtani K, Mizutani K, Hatono S, Kasai R, Sumino R, Shiota T. et al . Sanchinan-A, a reticuloendothelial system activating arabinogalactan from Sanchi-ginseng (roots of Panax notoginseng).  Planta Med. 1987;  53 166-9
  • 6 Sasaki R, Tsunoda S, Matano Y, Saito Y. Antitumor polysaccharides from Panax notoginseng roots. Japanese Patent Kokai Tokkyo Koho 02 268,120 1990
  • 7 Yamada H. Bioactive plant polysaccharides from Japanese and traditional herbal medicines. In: Paulsen BS, editor Bioactive carbohydrate polymers. Dordrecht Hardbound: Kluwer Academic Publishers 2000: p 15-24
  • 8 Tomoda M, Takeda K, Shimizu N, Gonda R, Ohara N, Takada K. et al . Characterisation of two novel polysaccharides having immunological activities from the root of Panax ginseng .  Biol Pharm Bull. 1993;  16 22-5
  • 9 Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N, Takada K. Characterisation of two novel polysaccharides having immunological activities from the root of Panax ginseng .  Biol Pharm Bull. 1993;  16 1087-90
  • 10 Sonoda S, Kasahara T, Mukaida N, Shimizu N, Tomoda M, Takeda T. Stimulation of interleukin-8 production by acidic polysaccharides from the root of Panax ginseng .  Immunopharmacology. 1997;  38 287-94
  • 11 Dubois M, Gilles K A, Hamilton J K, Rebers P A, Smith F. Colorimetric methods for determination of sugars and related substances.  Anal Chem. 1956;  18 350-6
  • 12 Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.  Anal Biochem. 1976;  72 248-54
  • 13 Shen Y C, Chou C J, Chiou W F, Chen C F. Anti-inflammatory effects of the partially purified extract of radix Stephaniae tetrandrae: comparative studies of its active principles tetrandrine and fangchinoline on human polymorphonuclear leukocyte functions.  Mol Pharmacol. 2001;  60 1083-90
  • 14 Kuo Y C, Yang N S, Chou C J, Lin L C, Tsai W J. Regulation of cell proliferation, gene expression, production of cytokines, and cell cycle progression in primary human T lymphocytes by piperlactam S isolated from Piper kadsura .  Mol Pharmacol. 2000;  58 1057-66
  • 15 Alban S, Classen B, Brunner G, Blaschek W. Differentiation between the complement modulating effects of an arabinogalactan-protein from Echinacea purpurea and heparin.  Planta Med. 2002;  68 1118-24
  • 16 Michaelsen T E, Gilje A, Samuelsen A B, Hogasen K, Paulsen B S. Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L.  Scand J Immunol. 2000;  52 483-90
  • 17 Leet C S, Vincan E, Thomas R J, Phillips W A. Lipopolysaccharide-induced priming of the human neutrophil is not associated with a change in phosphotyrosine phosphatase activity.  Int J Biochem Cell Biol. 1999;  31 585-93
  • 18 Ulmer A J, Flad H D, Rietschel T, Mattern T. Induction of proliferation and cytokine production in human T lymphocytes by lipopolysaccharide (LPS).  Toxicology. 2000;  152 37-45
  • 19 Zhang Y W, Kiyohara H, Matsumoto T, Yamada H. Fractionation and chemical properties of immunomodulating polysaccharides from roots of Dipsocus asperoides .  Planta Med. 1997;  63 393-9
  • 20 Hokputsa S, Harding S E, Inngjerdingen K, Jumel K, Michaelsen T E, Heinze T. et al . Bioactive polysaccharides from the stems of the Thai medicinal plant Acanthus ebracteatus: their chemical and physical features.  Carbohydr Res. 2004;  339 753-62
  • 21 Samuelsen A B. The traditional uses, chemical constituents, and biological activities of Plantago major. A review.  J Ethnopharmacol. 2000;  71 1-21
  • 22 Yamada H, Kiyohara H. Complement-activating polysaccharides from medicinal herbs. In: Wagner H, editor Immunomodulatory agents from plants. Basel; Birkhäuser Verlag 1999: p 161-202
  • 23 Ebringerova A, Kardosova A, Hromadkova Z, Hribalova V. Mitogenic and comitogenic activities of polysaccharides from some European herbaceous plants.  Fitoterapia. 2003;  74 52-61
  • 24 Dogasaki C, Murakami H, Nishijima M, Ohno N, Yadomae T, Miyazaki T. Biological activity and structural characterization of alkaline-soluble polysaccharides from the kernels of Prunus mume Sieb. et Zacc.  Biol Pharm Bull. 1994;  17 386-90
  • 25 Wagner H, Proksch A, Riess-Maurer I, Vollmar A, Odenthal S, Stuppner H. et al . Immunostimulating polysaccharides (heteroglycans) of higher plants.  Arzneimittelforschung. 1985;  35 1069-75
  • 26 Hokputsa S, Gerddit W, Pongsamart S, Inngjerdingen K, Heinze T, Koschella A. et al . Water-soluble polysaccharides with pharmaceutical importance from Durian rinds (Durio zibethinus Murr.): isolation, fractionation, characterization and bioactivity.  Carbohydr Polym. 2004;  56 471-81
  • 27 Gonda R, Tomoda M, Shimizu N, Yamada H. Structure and anti-complementary activity of an acidic polysaccharide from the leaves of Malva sylvestris var. mauritiana .  Carbohydr Res. 1990;  198 323-9
  • 28 Han S B, Yoon Y D, Ahn H J, Lee H S, Lee C W, Yoon W K. et al . Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus .  Int Immunopharmacol. 2003;  3 1301-12
  • 29 Thanavala Y, Mahoney M, Pal S, Scott A, Richter L, Natarajan N. et al . Immunogenicity in humans of an edible vaccine for hepatitis B.  Proc Natl Acad Sci USA. 2005;  102 3378-82

Professor A. Bacic

Cooperative Research Centre for Bioproducts

School of Botany

University of Melbourne

Victoria 3010

Australia

Phone: +61-3-8344-5041

Fax: +61-3-9347-1071

Email: abacic@unimelb.edu.au