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DOI: 10.1055/s-2007-981578
© Georg Thieme Verlag KG Stuttgart · New York
Plaunotol Induces Apoptosis of Gastric Cancer Cells
Publication History
Received: February 21, 2007
Revised: April 4, 2007
Accepted: May 8, 2007
Publication Date:
09 August 2007 (online)
Abstract
Although some isoprenoids, such as taxans and geranylgeraniol (GGOH), have been reported to have strong anticancer activities, the effect of plaunotol, the isoprenoid extracted from the leaves of Plau-noi, on cancer has not yet been evaluated. Here, we aimed to investigate the effect of plaunotol on gastric cancer cell lines. Three gastric cancer cell lines, namely MKN-45, MKN-74 and AZ-521 were used. Plaunotol was tested at 10, 20, 30 and 40 μmol/L. Plaunotol dose-dependently inhibited the growth of all gastric cancer cells, dependent on the induction of apoptosis. Caspases-8, -9 and -3, were found to be activated in the apoptotic cells. The expression of Bax protein was increased, but Bcl-2 and Bcl-xL protein expressions were not significantly affected. Plaunotol should be a promising new antitumor agent, and since it is already available for clinical use in Japan, its anticancer properties should be confirmed in clinical trials.
Abbreviations
COX: cyclooxygenase
DMSO: dimethyl sulfoxide
FITC: fluorescein isothiocyanate
GGOH: geranylgeraniol
MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium
PBS: phosphate-buffered saline
PG: prostaglandin
PI: propidium iodide
TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labelling
Key words
Plaunotol - cell apoptosis - isoprenoids - gastric cancer
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Jun Yamada
Department of Surgical Oncology
University of Tokyo
7-3-1 Hongo
Bunkyo-ku
Tokyo 113-8655
Japan
Phone: +81-3-3815-5411 (ext.37067)
Fax: +81-3-3811-6822
Email: jymda-tky@umin.ac.jp