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Planta Med 2007; 73(14): 1509-1516
DOI: 10.1055/s-2007-990260
Analytical Methods
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Simultaneous Determination of Eight Coumarins in Angelica gigas and in Various Other Angelica Species by High Performance Liquid Chromatography and Comparative Micro-Morphology Study of Angelica Species

Bharathi Avula1 , Vaishali C. Joshi1 , Niranjan Reddy V. L1 , Young-Whan Choi3 , Ikhlas A. Khan1 , 2
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS, USA
  • 2Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS, USA
  • 3Department of Horticulture, Miryang National University, Miryang, Korea
Further Information

Publication History

Received: May 21, 2007 Revised: September 27, 2007

Accepted: October 2, 2007

Publication Date:
16 November 2007 (online)

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Abstract

This article outlines the development of a simple and specific analytical method for the quantitative determination of eight coumarin constituents from the methanolic extract of the roots of Angelica gigas. The coumarin constituents present in the roots of Angelica gigas were separated using an acetonitrile (0.1 % acetic acid)-water (0.1 % acetic acid)-reagent alcohol gradient solvent system at a flow rate of 1.0 mL per minute. The HPLC separation was performed using a Phenomenex C12 reversed phase column with detection at 328 nm. The method was successfully used to study the percentage compositions of eight coumarins present in various Angelica species procured from S. Korea. Additionally, this manuscript provides a detailed microscopic account for the authentication of Angelica gigas and compared it with other Angelica species. The microscopic evaluation of plant tissue samples plays a vital role in botanical authentication. The combination of these two methodologies will provide additional tools for the evaluation of various Angelica species.

Key words

Angelica gigas - Apiaceae - coumarins - LC-UV - microscopy - micro-morphology

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Dr. Ikhlas A. Khan

National Center for Natural Products Research

Research Institute of Pharmaceutical Sciences

The University of Mississippi

University

MS 38677

USA

Phone: +1-662-915-7821

Email: ikhan@olemiss.edu