Semi-Automatic Blood Lactate Assay: Experiences from an Exercise Laboratory

J. Karlsson; I. Jacobs; B. Sjödin; P. Tesch; P. Kaiser; O. Sahl; B. Karlberg

doi:10.1055/s-2008-1026016

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 1983; 04(1): 52-55
DOI: 10.1055/s-2008-1026016

Technical Communication

Semi-Automatic Blood Lactate Assay: Experiences from an Exercise Laboratory

J. Karlsson, I. Jacobs, B. Sjödin, P. Tesch, P. Kaiser, O. Sahl, B. Karlberg

Depts. of Clinical Physiology and Environmental Medicine, Karolinska Institute; Swedish National Defence Research Institute; Biotest AB; Bifok AB, Stockholm, Sweden

Abstract

A semi-automatic system based on flow injection analysis (FIA) for the transportation of small sample aliquots has been combined with fluorometric, enzymatic methods for blood lactate determination and has been described earlier. In the present study duplicate blood samples were obtained from exercising subjects to enable a comparison of lactate concentrations between neutralized and non-neutralized samples. Duplicate samples were also obtained to enable FIA lactate values to be compared to those obtained with a manual enzymatic method, and with a colorimetric method. No significant change was observed if the sample was not neutralized, enabling a more rapid sample turnover. The FIA method was reliable, with a coefficient of variation of 4.9% between duplicate blood samples. FIA lactate values were valid when compared to two other manual assays. FIA has been shown to be a rapid (60 samples × h^-1 means of accurately determining blood lactate concentrations with 25 µl blood samples and is of particular relevance to the exercise laboratory.

Key words

blood lactate - flow injection analysis - exercise

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