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Planta Med 2008; 74(1): 84-89
DOI: 10.1055/s-2008-1034291
Analytical Studies
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Micellar Electrokinetic Chromatography for the Quantitative Analysis of Flavonoids in the Radix of Astragalus membranaceus var. mongholicus

Yue Song1 , Ping Li1 , Duo Wang1 , Yi-Yu Cheng1
  • 1Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou, People's Republic of China
  • 2Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, People's Republic of China
Further Information

Publication History

Received: May 16, 2007 Revised: October 25, 2007

Accepted: November 22, 2007

Publication Date:
17 January 2008 (online)

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Abstract

The separation and determination of eight flavonoids in the radix of Astragalus membranaceus (Fisch.) Bge var. mongholicus (Bge.) Hsiao was achieved by using micellar electrokinetic chromatography (MEKC) with a diode array detector (DAD). The influence of background electrolyte (BGE) concentrations and pH, surfactant concentrations, organic solvents, temperature, and voltage on the separate procedure was systematically investigated. The optimal resolution was obtained with a micellar phase consisting of 100 mM sodium cholate, 25 % (v/v) acetonitrile, 20 mM Na2B4O7, and 20 mM H3BO3 buffer at pH 9.2 by using a fused silica capillary at + 25 kV and 25 °C. A high reproducibility and good linearity (r2 > 0.9978) were obtained over the concentration range tested. The relative standard deviations (R.S.D.s) from intra-day and inter-day precision for injection were less than 4.42 %. Six plant samples from different locations were then analyzed for the flavonoids by this newly developed MEKC method.

Key words

Micellar electrokinetic chromatography - quantitative analysis - flavonoids - Leguminosae - Astragalus membranaceus var. mongholicus

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Yi-Yu Cheng

Pharmaceutical Informatics Institute

College of Pharmaceutical Sciences

Zhejiang University

Hangzhou 310027

People's Republic of China

Phone: +86-571-8795-2509

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Email: chengyy@zju.edu.cn