The Effects of Panduratin A Isolated from Kaempferia pandurata on the Expression of Matrix Metalloproteinase-1 and Type-1 Procollagen in Human Skin Fibroblasts

Abstract

Exposure of ultraviolet (UV) light on the skin induces photoaging associated with up-regulated matrix metalloproteinase (MMP) activities and decreased collagen synthesis. We investigated the effects of panduratin A isolated from Kaempferia pandurata Roxb. on the expression of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen in UV-irradiated human skin fibroblasts. Cultured human fibroblasts were irradiated with UV (20 mJ/cm²) and panduratin A was added into the medium of the fibroblast culture. The expressions of MMP-1 and type-1 procollagen levels were measured using Western blot analysis and RT-RCR. Panduratin A in the range of 0.001 - 0.1 μM significantly reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein and mRNA gene levels. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can be a potential candidate for the prevention and treatment of skin aging brought about by UV.

Abbreviations

UV:ultraviolet

MMP:matrix metalloproteinase

ROS:reactive oxygen species

DCFH-DA:2′,7′-dichlorofluorescein-diacetate

EGCG:epigallocatechin 3-O-gallate

RT-PCR:reverse transcriptase-polymerasechain reaction

Key words

Kaempferia pandurata - Zingiberaceae - panduratin A - UV irradiation - matrix metalloproteinase-1 - type-1 procollagen

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