Comparisons of Scutellaria baicalensis, Scutellaria lateriflora and Scutellaria racemosa: Genome Size, Antioxidant Potential and Phytochemistry

Abstract

The genus Scutellaria in the family Lamiaceae has over 350 species, many of which are medicinally active. One species, Scutellaria baicalensis, is one of the most widely prescribed plants in Traditional Chinese Medicine, used for neurological disorders, cancer and inflammatory diseases and has been the subject of detailed scientific study but little is known about the phytochemistry of other Scutellaria. The current study was designed to compare the medicinal phytochemistry of 3 species of Scutellaria used to treat neurological disorders. To accomplish this objective, the specific objectives were (a) to establish an in vitro collection of the South American native; S. racemosa, (b) to botanically characterize S. racemosa and (c) to compare the phytochemistry of S. racemosa with S. baicalensis and S. lateriflora. S. racemosa was established in vitro from wild populations in Florida. Botanically, S. racemosa is diploid with 18 chromosomes, and flow cytometry data indicated that S. baicalensis and S. racemosa have small nuclei with estimated small genomes (377 mbp and 411 mbp respectively). Antioxidant potential studies showed that there were no significant differences in the 3 Scutellaria species. Phytochemical analyses detected and quantified the flavonoids baicalin, baicalein, scutellarin, and wogonin as well as the human neurohormones melatonin and serotonin in leaf and stem tissues from S. baicalensis, S. lateriflora, and S. racemosa. These findings represent the first phytochemical analysis of S. racemosa and establish S. racemosa as a model system for study of medicinal plant secondary metabolism and as a potential source of important phytopharmaceuticals for treatment of human disease.

Scutellaria - Lamiaceae - melatonin - baicalin - wogonin - antioxidant

References

• 1 Cole I B, Saxena P K, Murch S J. Medicinal biotechnology in the genus Scutellaria. .  In Vitro Cell Dev Biol Plant. 2007;  43 318-27
• 2 Watanabe H, Kobayashi T, Meibou T, Sekiguchi Y, Uchida K, Aoki T. et al . Effects of Kampo herbal medicine on plasma melatonin concentration in patients.  Am J Chin Med. 2002;  30 65-71
• 3 Foster S. Scullcap: A herbal enigma.  The Business of Herb. 1996;  3 14-6
• 4 Awad R, Amason J T, Trudeau V, Bergeron C, Budzinski J W, Foster B C. et al . lPhytochemical and biological analysis of skullcap (Scutellaria lateriflora L.): A medicinal plant with anxiolytic properties.  Phytomedicine. 2003;  10 640-9
• 5 Gafner S, Bergeron C, Batcha L L, Reich J, Arnason J T, Burdette J E. et al . Inhibition of [³H]-LSD binding to 5-HT7 receptors by flavonoids from Scutellaria lateriflora. .  J Nat Prod. 2003;  66 535-7
• 6 Bianchi A. Attivita antidepressiva di due specie di Scutellariae Colombiane.  Annual Meeting for the Instruction of Acupuncture and Reflexology. Torino; 2006
• 7 Murch S J, Simmons C B, Saxena P K. Melatonin in feverfew and other medical plants.  Lancet. 1997;  350 1598-9
• 8 Murch S J, Rupasinghe H PV, Goodenowe D, Saxena P K. A metobolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi.) genotypes discovery of novel compounds.  Plant Cell Rep. 2004;  23 419-25
• 9 Han J, Ye M, Xu M, Sun J, Wang B, Guo D. Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry.  J Chromatogr B Biomed Sci Appl. 2007;  848 355-62
• 10 de Boer J G, Quiney B, Murch S J, Saxena P K. Protection against aflatoxin-B1-induced liver mutagenesis by Scutellaria baicalensis. .  Mutat Res. 2005;  578 15-22
• 11 Tobe J D, Craddock Burks K, Cantrell R W, Garland M A, Sweeley M E, Hall D W. Florida wetland plants: an identification manual. Tallahassee; Florida Department of Environmental Protection 1998
• 12 Krings A, Neal J C. South American skullcap (Scutellaria racemosa: Lamiaceae) in the Southeastern United States.  SIDA. 2001;  19 1171-9
• 13 Li H, Murch S J, Saxena P K. Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls, and stem segments of Huang-qin (Scutellaria baicalensis).  Plant Cell Tiss Org Cult. 2000;  62 169-73
• 14 Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures.  Physiol Plant. 1962;  15 473-8
• 15 Gamborg O L, Miller R A, Ojima K. Nutrient requirement of a suspension cultures of soybean root cells.  Exp Cell Res. 1968;  50 150-8
• 16 Wunderlin R P, Hansen B F. Atlas of Florida vascular plant. Institute for Systematic Botany: University of South Florida Press 2004
• 17 Alan A R, Zeng H, Assani A, Shi W L, McRae H E, Murch S J. et al . Assessment of genetic stability of the germplasm lines of medicinal plant Scutellaria baicalensis Georgi. (Huang-qin) in long term, in vitro maintained cultures.  Plant Cell Rep. 2007;  26 1345-55
• 18 Liu C Z, Murch S J, El-Demerdash M, Saxena P K. Artemisia judaica L.: micropropagation and antioxidant activity.  J Biotechnol. 2004;  110 63-71
• 19 Cao J, Murch S J, O’Brien R, Saxena P K. Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry.  J Chromatogr A. 2006;  1134 333-7
• 20 Murch S J, Krishnaraj S, Saxena P K. Phytopharmaceuticals: mass-production, standardization, and conservation.  Sci Rev Alt Med. 2000;  4 33-8
• 21 Gao S J, Chen B J, Zhu D N. In vitro production and identification of autotetraploids of Scutellaria baicalensis. .  Plant Cell Tissue Organ Cult. 2002;  70 289-93
• 22 Gill L S, Morton J K. Scutellaria churchilliana-hybrid or species?.  Syst Bot. 1978;  3 342-8
• 23 Wojcikowski K, Stevenson L, Leach D, Wohlmuth H, Gobe G. Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: A comparison using a sequential three-solvent extraction process.  J Alt Compl Med. 2007;  13 103-9
• 24 Gao D, Sakurai K, Katoh M, Chen J, Ogiso T. Inhibition of microsomal lipid peroxidation by baicalein: A possible formation of an iron-baicalein complex.  Biochem Mol Biol Int. 1996;  39 215-25
• 25 Murch S J, Saxena P K. A melatonin-rich germplasm line of St John′s wort (Hypericum perforatum L.).  J Pineal Res. 2006;  41 284-7
• 26 Horvath C R, Martos P A, Saxena P K. Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi.) using high-performance liquid chromatography with diode array and mass spectrometric detection.  J Chromatogr A. 2005;  1062 199-207
• 27 Zhang L, Zhang R W, Li Q, Lian J -W, Liang J, Chen X -H. et al . Development of the fingerprints for the quality evaluation of Scutellariae Radix by HPLC-DAD and LC-MS-MS.  Chromatographia. 2007;  66 13-20
• 28 Tai M C, Tsand S Y, Chang L YF, Xue H. Therapeutic potential of wogonin: A naturally occurring flavonoid.  CNS Drug Rev. 2005;  11 41-50
• 29 Chen G F, Huo Y S, Tan D X, Liang Z, Zhang W, Zhang Y. Melatonin in Chinese medicinal herbs.  Life Sci. 2003;  73 19-26
• 30 Arnao M B, Hernandez-Ruiz J. The physiological function of melatonin in plants.  Plant Signal Behav. 2006;  1 89-95