Contrast medium attenuates platelet activation and platelet-leukocyte cross–talk

Qiushang Ji; Melania Ghaly; Paul Hjemdahl; Per Tornvall; Nailin Li

doi:10.1160/TH04-12-0809

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 93(05): 922-926
DOI: 10.1160/TH04-12-0809

Platelets and Blood Cells

Schattauer GmbH

Contrast medium attenuates platelet activation and platelet-leukocyte cross–talk

Qiushang Ji

¹Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden

³Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China

,

Melania Ghaly

¹Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden

,

Paul Hjemdahl

¹Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden

,

Per Tornvall

²Cardiology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden

,

Nailin Li

¹Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden

› Author Affiliations

Abstract

Summary

The influence of ionic and non-ionic contrast media (CM) on platelet and leukocyte activation and platelet-leukocyte crosstalk was investigated in hirudinized whole blood. The blood was incubated with and without the ionic CM ioxaglate and the nonionic CM iodixanol at 37ºC for 5 min, without or with stirring. Platelet and leukocyte activation and platelet-leukocyte aggregation were measured using whole blood flow cytometry. When blood samples were pre-incubated in the presence of 2%, 5%, and 10% of CM without stirring, both ioxaglate and iodixanol had little effect on unstimulated samples, but dose-independently decreased 1 μM ADP-induced platelet P-selectin expression and fibrinogen binding, and thus platelet-leukocyte aggregate formation. Ioxaglate had little effect on leukocyte CD11b expression, whilst iodixanol slightly enhanced resting and N-formyl-methionyl-leucyl-phenylalanine (fMLP; 0.1 μM)-stimulated leukocyte CD11b expression. Blood samples were also incubated with stirring to investigate the impact of CM (5% of ioxaglate or iodixanol) on platelet-leukocyte cross-talk. Collagen induced marked platelet activation and platelet-leukocyte aggregation, and subsequently elevated leukocyte CD11b expression. The latter was attenuated by ioxaglate and iodixanol, and was accompanied by reduced platelet-leukocyte aggregation. In conclusion, the CM ioxaglate and iodixanol attenuate platelet activation and platelet-leukocyte cross-talk. Inhibitory effects of the contrast agents on this cross-talk are apparently exerted by reducing heterotypic conjugation, and may be beneficial in connection with PCI.

Keywords

Contrast media - platelets - leukocytes - platelet-leukocyte cross-talk

Full Text

References

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