Endothelial cells express a matrix protein which binds activated factor XII in a zinc-independent manner

Summary

Recent studies have shown that peptides identified as surface binding regions of high molecular mass kininogen (HK) and factor XII (FXII) inhibit the Zn²⁺-dependent binding of FXII to confluent layers of human umbilical vein endothelial cells (HUVEC). This indicates that negatively charged FXII binding surfaces, such as sulfatides and dextran sulfate, may interfere with the binding of FXII to confluent layer of HUVEC. Upon investigating this hypothesis it was unexpectedly found that sulfatides enhanced a specific binding of FXII to a matrix protein expressed during growth of the endothelial cells and that this binding was independent of the presence of Zn²⁺. The function of sulfatides was partly to minimize nonspecific electrostatic binding and partly to induce and enhance autoactivation of FXII generating αFXIIa. Western blot analysis of the extracts of the matrix incubated with FXII and sulfatides showed that the binding was specific for αFXIIa. The dissociation constant for binding αFXIIa was 12. 8 ± 0. 4 nM (n=4). The binding of αFXIIa to ECM was mapped to the heavy chain as no binding was observed of the light chain containing the catalytic domain. HK, which previously has been shown to completely abolish the Zn²⁺-dependent binding of FXII to confluent layers of HUVEC, did not inhibit the binding of αFXIIa to the matrix but sulfatides enhanced binding of FXII to ECM. This suggests that HK interferes with the binding of FXII to sulfatides and thereby the autoactivation of FXII. Trypsin treatment of the matrix protein completely abolished the binding, and fibronectin but not laminin was found to bea suitable target. The binding of activated FXII to the ECM suggests that FXIIa may bea modulator of cellular adhesion, migration and vascularization.

Keywords

Factor XII - endothelial cell matrix - HUVEC - sulfatides - zinc

• References

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