Plasmin/plasminogen is essential for the healing of tympanic membrane perforations

Jinan Li; Per-Olof Eriksson; Annika Hansson; Sten Hellström; Tor Ny

doi:10.1160/TH06-03-0168

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 96(04): 512-519
DOI: 10.1160/TH06-03-0168

Wound Healing and Inflammation/Infection

Schattauer GmbH

Plasmin/plasminogen is essential for the healing of tympanic membrane perforations

Jinan Li

¹Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden

,

Per-Olof Eriksson

²Department of Clinical Sciences, Otorhinolaryngology, Umeå University, Umeå, Sweden

,

Annika Hansson

²Department of Clinical Sciences, Otorhinolaryngology, Umeå University, Umeå, Sweden

,

Sten Hellström

²Department of Clinical Sciences, Otorhinolaryngology, Umeå University, Umeå, Sweden

,

Tor Ny

¹Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden

› Author Affiliations

Abstract

Summary

Plasminogen has been proposed to play an important role in different tissue remodeling processes such as wound healing and tissue regeneration after injuries. The healing of tympanic membrane perforations is a well-organized chain of inflammatory events, with an initial invasion of inflammatory cells followed by reparative and restoration phases. Here we show that the healing of tympanic membrane perforations is completely arrested in plasminogen-deficient mice, with no signs of any healing even 143 days after perforation. Inflammatory cells were recruited to the wounded area, but there were no signs of tissue debridement. In addition, removal of fibrin, keratinocyte migration and in-growth of connective tissue were impaired. This contrasts with skin wound healing, where studies have shown that, although the healing process is delayed, it reaches completion in all plasminogen-deficient mice. Our finding that keratinocyte migration and re-epithelialization were completely arrested in plasminogen-deficient mice indicates that plasminogen/plasmin plays a more profound role in the healing of tympanic membrane perforations than in the healing of other epithelial wounds.

Keywords

Plasmin - wound healing - tympanic membrane - inflammation - neutrophils

Full Text

References

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