Effect of an anti-sulfatide single-chain antibody probe on platelet function

Prasenjit Guchhait; Corie N. Shrimpton; Koichi Honke; Rolando E. Rumbaut; Jose A. Lopez; Perumal Thiagarajan

doi:10.1160/TH07-05-0351

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 99(03): 552-557
DOI: 10.1160/TH07-05-0351

Platelets and Blood Cells

Schattauer GmbH

Effect of an anti-sulfatide single-chain antibody probe on platelet function

Prasenjit Guchhait

¹Thrombosis Research Section, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA

,

Corie N. Shrimpton

¹Thrombosis Research Section, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA

,

Koichi Honke

²Department of Biochemistry, Kochi University Medical School, Kochi, Japan

,

Rolando E. Rumbaut

³Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA

,

Jose A. Lopez

⁴Research Division, Puget Sound Blood Center, Seattle, Washington, USA

,

Perumal Thiagarajan

¹Thrombosis Research Section, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA

³Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA

› Author Affiliations

Abstract

Summary

Sulfatide (galactocylceramide-3'-sulfate), a cell surface glycosphingolipid interacts with several cell adhesion molecules including fibrinogen, von Willebrand factor (VWF), P-selectin, thrombospondin (TSP) and laminin, which are involved in haemostasis.We have used a sulfatide-specific single-chain fragment variable (scFv) antibody probe PA38 and sulfatide-deficient mice to investigate the role of membrane sulfatide in platelet function. PA38 bound to platelets and binding increased following platelet activation. Sulfatide was localized as a large cluster towards the center of the platelet surface when examined in a confocal microscope. PA38 (20 μg/ml) inhibited the adhesion of activated platelets to fibrinogen,VWF, P-selectin,TSP1 and laminin by 30%, 30%,75%,20% and 35%,respectively,compared to a control scFv (p<0.05). Furthermore, PA38 inhibited collagen, ADP, thrombin and ristocetin-induced platelet aggregation in PRP by 25%, 30%, 18% and 20%, respectively, compared to the control scFv (p<0.05). In a PFA-100 platelet function assay, PA38 prolonged the occlusion time by 25% (p<0.05).Under flow PA38 decreased the thrombus formation on collagen by 31%, (p<0.01). Sulfatidedeficient mice displayed an extended lag-phase in collagen-induced platelet aggregation compared to wild type (p<0.05), though in-vivo haemostasis did not differ significantly.Thus, this study provides new evidence for a role for membrane sulfatide in platelet function.

Keywords

Sulfatide - anti-sulfatide scFv - platelet function

Full Text

References

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