Unravelling the mechanism and significance of thrombin binding to platelet glycoprotein Ib

Zaverio M. Ruggeri; Alessandro Zarpellon; James R. Roberts; Richard A. Mc Clintock; Hua Jing; G. Loredana Mendolicchio

doi:10.1160/TH10-09-0578

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2010; 104(05): 894-902
DOI: 10.1160/TH10-09-0578

Theme Issue Article

Schattauer GmbH

Unravelling the mechanism and significance of thrombin binding to platelet glycoprotein Ib

Authors

Zaverio M. Ruggeri

¹Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
Alessandro Zarpellon

¹Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
James R. Roberts

¹Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
Richard A. Mc Clintock

¹Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
Hua Jing

¹Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
G. Loredana Mendolicchio

²IRCCS Istituto Clinico Humanitas, Milan, Italy

Abstract

Summary

The main question concerning the mechanism of α-thrombin binding to platelet membrane glycoprotein (GP)Ib is whether it involves both thrombin exosite I and exosite II. The solution of two independent crystal structures suggests alternative explanations that may actually reflect different modes of binding with distinct pathophysiological significance. With respect to function, it is still unclear whether thrombin binding to GPIb promotes procoagulant and prothrombotic pathways of re-sponse to vascular injury or limits such responses by sequestering, at least temporarily, the active enzyme. We review here published information on these topics and touch upon ongoing studies aimed at finding definitive answers to outstanding questions relevant for a better understanding of thrombosis and haemostasis.

Keywords

Thrombin - GPIb - von Willebrand factor - thrombosis - animal models

Full Text

References

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