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Thromb Haemost 2011; 105(05): 783-789
DOI: 10.1160/TH10-10-0635
Theme Issue Article
Schattauer GmbH

Imaging inflammatory plasma leakage in vivo

Ellinor Kenne
1   Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
,
Lennart Lindbom
1   Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
› Author Affiliations Financial support:The authors work is funded by grants from the Swedish Research Council, the Swedish Heart-Lung Foundation, Swedish Foundation for Strategic Research, and the Lars Hierta Memorial Fund.
Further Information

Publication History

Received: 06 October 2010

Accepted after minor revision: 10 February 2011

Publication Date:
28 November 2017 (online)

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Summary

Increased vascular permeability and consequent plasma leakage from postcapillary venules is a cardinal sign of inflammation. Although the movement of plasma constituents from the vasculature to the affected tissue aids in clearing the inflammatory stimulus, excessive plasma extravasation can lead to hospitalisation or death in cases such as influenza-induced pneumonia, burns or brain injury. The use of intravital imaging has significantly contributed to the understanding of the mechanisms controlling the vascular permeability alterations that occur during inflammation. Today, intravital imaging can be performed using optical and non-optical techniques. Optical techniques, which are generally used in experimental settings, include traditional intravital fluorescence microscopy and near-infrared fluorescence imaging. Magnetic resonance (MRI) and radioisotopic imaging are used mainly in the clinical setting, but are increasingly used in experimental work, and can detect plasma leakage without optics. Although these methods are all able to visualise inflammatory plasma leakage in vivo, the spatial and temporal resolution differs between the techniques. In addition, they vary with regards to invasiveness and availability. This overview discusses the use of imaging techniques in the visualisation of inflammatory plasma leakage.

Keywords

Inflammation - imaging - endothelial cells
 

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