Thromb Haemost 2012; 107(06): 1185-1187
DOI: 10.1160/TH11-07-0509
Letters to the Editor
Schattauer GmbH

Correlation between platelet-derived microparticle enumeration by flow cytometry and phospholipid-dependent procoagulant activity in microparticles: The centrifugation step matters!

Jérémie Stagnara*
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Francine Garnache Ottou
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Fanny Angelot
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Guillaume Mourey
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Estelle Seilles
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Sabéha Biichlé
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
,
Philippe Saas
1   INSERM, UMR1098, Besançon Cedex, France
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
4   CHU Besançon, Centre d’Investigation Clinique intégré en Biothérapies, CBT506, Besançon Cedex, France
,
Evelyne Racadot
2   Université de Franche-Comté, Besançon Cedex, France
3   EFS Bourgogne Franche-Comté, laboratoires d’hémostase, cytologie, plateforme de biomonitoring, Besançon Cedex, France
› Author Affiliations
Financial support: This work is supported by a grant from the Etablissement Français du Sang (French Blood Agency depending on the French Ministry of Health, APR 2011, grant #2011–11 to PS).
Further Information

Publication History

Received: 08 August 2011

Accepted after major revision: 24 February 2012

Publication Date:
29 November 2017 (online)

 

* Current address: Hopital de la Croix-Rousse, Laboratoire d'hématologie, 69317 Cedex 04, France.


 
  • References

  • 1 Burnier L, Fontana P, Kwak BR. et al. Cell-derived microparticles in haemostasis and vascular medicine. Thromb Haemost 2009; 101: 439-451.
  • 2 Angelot F, Seilles E, Biichle S. et al. Endothelial cell-derived microparticles induce plasmacytoid dendritic cell maturation: potential implications in inflammatory diseases. Haematologica 2009; 94: 1502-1512.
  • 3 Thery C, Ostrowski M, Segura E. Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 2009; 9: 581-593.
  • 4 Leroyer AS, Anfosso F, Lacroix R. et al. Endothelialderived microparticles: Biological conveyors at the crossroad of inflammation, thrombosis and angiogenesis. Thromb Haemost 2010; 104: 456-463.
  • 5 Tsimerman G, Roguin A, Bachar A. et al. Involvement of microparticles in diabetic vascular complications. Thromb Haemost 2011; 106: 310-321.
  • 6 Connor DE, Exner T, Ma DD. et al. The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein Ib. Thromb Haemost 2010; 103: 1044-1052.
  • 7 Yuana Y, Bertina RM, Osanto S. Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb Haemost 2011; 105: 396-408.
  • 8 Robert S, Poncelet P, Lacroix R. et al. Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?. J Thromb Haemost 2009; 7: 190-197.
  • 9 Pelletier F, Garnache-Ottou F, Angelot F. et al. Increased levels of circulating endothelial-derived microparticles and small-size platelet-derived microparticles in psoriasis. J Invest Dermatol 2011; 131: 1573-1576.
  • 10 Connor DE, Exner T, Ma DD. et al. Detection of the procoagulant activity of microparticle-associated phosphatidylserine using XACT. Blood Coagul Fibrinolysis 2009; 20: 558-564.