Regulation of STIM1/Orai1-dependent Ca2+ signalling in platelets

Florian Lang; Patrick Münzer; Meinrad Gawaz; Oliver Borst

doi:10.1160/TH13-02-0176

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2013; 110(05): 925-930
DOI: 10.1160/TH13-02-0176

Theme Issue Article

Schattauer GmbH

Regulation of STIM1/Orai1-dependent Ca²⁺ signalling in platelets

Florian Lang

¹Department of Physiology, University of Tübingen, Germany

,

Patrick Münzer

¹Department of Physiology, University of Tübingen, Germany

,

Meinrad Gawaz

²Department of Cardiology & Cardiovascular Medicine, University of Tübingen, Germany

,

Oliver Borst

¹Department of Physiology, University of Tübingen, Germany

²Department of Cardiology & Cardiovascular Medicine, University of Tübingen, Germany

› Author Affiliations

Abstract

Summary

Platelet secretion and aggregation as well as thrombus formation of blood platelets critically depend on increase of cytosolic Ca²⁺ concentration ([Ca²⁺]i) mainly resulting from intracellular Ca²⁺ release followed by store operated Ca²⁺ entry (SOCE) through Ca²⁺ release activated channels (CRAC). SOCE is in part accomplished by the pore forming unit Orai and its regulator stromal interaction molecule (STIM). Orai1 and STIM1 transcription is stimulated by NFkB (nuclear factor kappa B). Serum- and glucocorticoid-inducible kinase 1 (SGK1) up-regulates NFkB-activity in megakaryocytes and thus Orai1- expression and SOCE in platelets. SGK1 is thus a powerful regulator of platelet Ca²⁺-signalling and thrombus formation and presumably participates in the regulation of platelet activation by a variety of hormones as well as clinical conditions (e.g. type 2 diabetes or metabolic syndrome) associated with platelet hyperaggregability and increased risk of thromboocclusive events. SOCE in platelets is further regulated by scaffolding protein Homer and chaperone protein cyclophilin A (CyPA). Additional potential regulators of Orai1/STIM1 and thus SOCE in platelets include AMP activated kinase (AMPK), protein kinase A (PKA), reactive oxygen species, lipid rafts, pH and mitochondrial Ca²⁺ buffering. Future studies are required defining the significance of those mechanisms for platelet Orai1 abundance and function, for SOCE into platelets and for platelet function in cardiovascular diseases.

Keywords

Platelet physiologypolymorphisms - kinases - signal transduction - Ca²⁺ (arterial thrombosis)

Full Text

References

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