MiR-223 is dispensable for platelet production and function in mice

Simon Leierseder; Tobias Petzold; Lin Zhang; Xavier Loyer; Steffen Massberg; Stefan Engelhardt

doi:10.1160/TH13-07-0623

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2013; 110(06): 1207-1214
DOI: 10.1160/TH13-07-0623

Platelets and Blood Cells

Schattauer GmbH

MiR-223 is dispensable for platelet production and function in mice

Simon Leierseder

¹Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany

,

Tobias Petzold^*

²Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany

,

Lin Zhang^*

²Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany

³Current affiliations: Heart Failure Institute, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China

⁴Current affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China

,

Xavier Loyer

¹Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany

⁵Current affiliation: Inserm U970, Paris Cardiovascular Research Center, Paris, France

,

Steffen Massberg

²Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany

⁶DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

,

Stefan Engelhardt

¹Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany

⁶DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

› Author Affiliations

Abstract

Summary

MicroRNAs (miRNAs) are key physiological regulators in multiple cell types. Here, we assessed platelet production and function in mice deficient in miR-223, one of the most abundantly expressed miRNAs in platelets and megakaryocytes. We found platelet number, size, lifespan as well as surface expression of platelet adhesion receptors to be unchanged in miR-223-deficient mice. Likewise, loss of miR-223 did not affect platelet activation, adhesion and aggregation and also had no effect on bleeding times. Moreover, miR-223 null megakaryocytes developed normally and were capable to form pro-platelets. However, we detected a transient delay in the recovery of platelet numbers following antibody-induced platelet depletion in miR-223-deficient animals. This delay was not observed after transplantation of bone marrow from miR-223-deficient animals into wild-type recipients, indicating a non-cell-autonomous role of miR-223 for thrombopoiesis. Overall, our data indicate a surprisingly modest role of miR-223 in platelet production, while the function of platelets does not seem to depend on miR-223.

Keywords

MicroRNA - platelets - megakaryocytes - transgenic mice

Full Text

References

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