Performance of two new automated assays for measuring von Willebrand activity: HemosIL AcuStar and Innovance

Emmanuel de Maistre; Fabienne Volot; Guillaume Mourey; Ludwig S. Aho; Catherine Ternisien; Marie-Elisabeth Briquel; Marie-Anne Bertrand; Brigitte Tardy; Birgit Frotscher; Philippe Nguyen; Laure Dumont; David Vandroux; Nathalie Hezard; Marc Trossaërt

doi:10.1160/TH14-02-0108

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2014; 112(04): 825-830
DOI: 10.1160/TH14-02-0108

New Technologies, Diagnostic Tools and Drugs

Schattauer GmbH

Performance of two new automated assays for measuring von Willebrand activity: HemosIL AcuStar and Innovance

Authors

Emmanuel de Maistre

¹Haematology Laboratory, University Hospital, Dijon, France
Fabienne Volot

²Haemophilia Center, University Hospital, Dijon, France
Guillaume Mourey

³Etablissement Français du Sang, Besançon, France
Ludwig S. Aho

⁴Epidemiology Department, University Hospital, Dijon, France
Catherine Ternisien

⁵Haematology Laboratory, University Hospital, Nantes, France
Marie-Elisabeth Briquel

⁶Haemophilia Center, University Hospital, Nancy, France
Marie-Anne Bertrand

⁷Haemophilia Center, University Hospital, Besançon, France
Brigitte Tardy

⁸Haematology Laboratory, University Hospital, Saint Etienne, France
Birgit Frotscher

⁶Haemophilia Center, University Hospital, Nancy, France
Philippe Nguyen

⁹Haematology Laboratory, University Hospital, Reims, France
Laure Dumont

¹⁰NVH Medicinal, Dijon, France
David Vandroux

¹⁰NVH Medicinal, Dijon, France
Nathalie Hezard

⁹Haematology Laboratory, University Hospital, Reims, France
Marc Trossaërt

⁵Haematology Laboratory, University Hospital, Nantes, France

Abstract

Summary

The ristocetin cofactor activity assay (VWF:RCo) is the reference method for assessing von Willebrand factor (VWF) activity but remains difficult to perform, and the coefficient of variation of the method is high (about 20–30%). This study evaluated and compared the performance for measuring the VWF activity of two newly commercialised assays [VWF:Ac Innovance (VWF:Ac) and VWF:RCo Acustar (VWF:RCo Acu)] with the reference VWF:RCo aggregation in 123 pathological plasma samples. The correlation and concordance between both new tests (VWF:RCo-Acu and VWF:Ac) and the reference VWF:RCo were good. The results of the VWF activity to VWF antigen ratio were also comparable whatever the method for the classification of VWF deficiency in all patients. Our results showed that both new tests could replace the “gold standard” VWF:RCo in aggregometry with several benefits: they are fully automated, easier and faster to perform, better adapted to emergency situations if necessary.

Keywords

Willebrand activity assay - ristocetin cofactor activity - aggregometry - von Willebrand disease

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Referenzen

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