Moderate oral supplementation with docosahexaenoic acid improves platelet function and oxidative stress in type 2 diabetic patients

Evelyne Véricel; Romain Colas; Catherine Calzada; Quang Huy Lê; Nathalie Feugier; Christine Cugnet; Hubert Vidal; Martine Laville; Philippe Moulin; Michel Lagarde

doi:10.1160/TH14-12-1003

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2015; 114(02): 289-296
DOI: 10.1160/TH14-12-1003

Cellular Haemostasis and Platelets

Schattauer GmbH

Moderate oral supplementation with docosahexaenoic acid improves platelet function and oxidative stress in type 2 diabetic patients

Evelyne Véricel

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

,

Romain Colas

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

,

Catherine Calzada

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

,

Quang Huy Lê

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

,

Nathalie Feugier

²Centre de Recherche en Nutrition Humaine Rhône-Alpes, Pierre-Bénite, France

,

Christine Cugnet

³Hôpital Cardiovasculaire Louis Pradel, Lyon-Bron, France

,

Hubert Vidal

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

,

Martine Laville

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

²Centre de Recherche en Nutrition Humaine Rhône-Alpes, Pierre-Bénite, France

,

Philippe Moulin

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

³Hôpital Cardiovasculaire Louis Pradel, Lyon-Bron, France

,

Michel Lagarde

¹Université de Lyon, Inserm UMR 1060, Laboratoire CarMeN, Université Claude Bernard Lyon 1, INSA-Lyon, Villeurbanne, France

› Author Affiliations

Abstract

Summary

Platelets from patients with type 2 diabetes are characterised by hyperactivation and high level of oxidative stress. Docosahexaenoic acid (DHA) may have beneficial effects on platelet reactivity and redox status. We investigated whether moderate DHA supplementation, given as a triglyceride form, may correct platelet dysfunction and redox imbalance in patients with type 2 diabetes. We conducted a randomised, double-blind, placebo-controlled, two-period crossover trial (n=11 post-menopausal women with type 2 diabetes) to test the effects of 400 mg/day of DHA intake for two weeks on platelet aggregation, markers of arachidonic acid metabolism, lipid peroxidation status, and lipid composition. Each two week-period was separated from the other by a six-week washout. Daily moderate dose DHA supplementation resulted in reduced platelet aggregation induced by collagen (-46.5 %, p< 0.001), and decreased platelet thromboxane B₂ (-35 %, p< 0.001), urinary 11-dehydro-thromboxane B₂ (-13.2 %, p< 0.001) and F2-isoprostane levels (-19.6 %, p< 0.001) associated with a significant increase of plasma and platelet vitamin E concentrations (+20 % and +11.8 %, respectively, p< 0.001). The proportions of DHA increased both in plasma lipids and in platelet phospholipids. After placebo treatment, there was no effect on any parameters tested. Our findings support a significant beneficial effect of low intake of DHA on platelet function and a favourable role in reducing oxidative stress associated with diabetes.

Keywords

Platelet aggregation - arachidonic acid - vitamin E - isoprostane - plasma and platelet lipid composition

Full Text

References

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