RESULTS
Anatomical considerations
Peripheral nerves are formed by parallel bundles (fascicles) embedded in a collagenous
matrix (epineurium). Each fascicle is separated by a perineural sheath formed by flattened
squamous cells connected by tight junctions (perineurium). The individual motor, sensory
and autonomic nerve fiber (axon and its associated Schwann cells) is enveloped by
connective tissue (endoneurium), although some authors include the Schwann cells and
axons as part of the endoneurium[2 ]. In addition to different functional subtypes (motor, sensory and autonomic), axons
can also be distinguished by different degrees of myelination, being highly, moderately,
or thinly myelinated (Aδ fibers), or completely lacking a myelin envelope, the so-called
unmyelinated fibers.
Unmyelinated fibers (type C) can be autonomic or sensory. They are surrounded by Schwann
cells in a different way, forming the so-called Remak fibers. In 1838, Remak teased
autonomic nerve fibers in water and differentiated myelinated (tubuli primitivi ) from unmyelinated fibers – fibrae organicae
[3 ]. In 1868, Langerhans described the intra-epidermal nerve fiber endings[4 ]. Sixty years later, Remak and Tuckett identified both Schwann cells and axons[3 ]. Joseph Erlanger and Herbert Spencer Gasser won the Nobel Prize in 1944 for their
studies on the differential functions of single nerve fibers. Unmyelinated axons range
in diameter from 0.2 μm to 3 μm and in men there are 1–4 axons per Schwann cell unit[3 ]. The proportion of myelinated to unmyelinated fibers in the sural nerve is 1:4[5 ]. The presence of myelin in very thin fibers does not increase the conduction speed
and its absence makes unmyelinated fibers more resistant to metabolic insults[3 ]. On the other hand, since unmyelinated fibers are not insulated from each other,
impulses conducting in one axon may spread to neighboring axons[3 ].
New histological evidence from skin biopsies completely changed the concept of SFN,
as it revealed that axons lose the Schwann cell ensheathment as they cross the dermal-epidermal
junction[6 ]. However, since the loss of distal nerve endings may indicate the loss of distal
fibers from more prominently myelinated sensory fibers, distal nerve fiber loss may
be due to a less specific sensory involvement, which could include myelinated sensory
fibers (e.g. mediating vibration and proprioception) amenable to assessment by electromyography.
This controversial aspect will be discussed further in the next sections.
Definition and clinical subtypes of small fiber neuropathy
The term “small fiber neuropathy” was first employed by Stewart et al.[7 ] From the clinical point of view, SFN is defined as a syndrome characterized by sensory
or combined sensory and autonomic manifestations, associated with an almost entirely
normal neurological examination (except for possible distal pinprick and thermal loss)
or at least abnormalities solely associated with small fiber involvement (e.g. orthostatic
hypotension).
Small fiber neuropathy may present as negative and positive manifestations. For the
sensory subtype of SFN, positive manifestations usually dominate the clinical picture.
Patients may complain of dysesthesias, burning, stinging, tingling sensations and
neuropathic pain (length-dependent), usually more severe in the evening. Additional
complaints include formication-like phenomena and chronic pruritus[8 ]. Patchy or non-length dependent symptoms have also been described.
The exclusive autonomic subtype has a broader range of manifestations, that can be
easily mimicked by several other medical conditions, such as dizziness (due to orthostatic
intolerance or orthostatic hypotension), syncopal spells, anhidrosis or hyperhidrosis,
decreased lacrimation, decreased salivation, important trophic skin changes, changes
in bowel habits (constipation or diarrhea) or urinary complaints (polyuria, urinary
retention or incontinence), among others ( for a more extensive review of autonomic
manifestations, the reader is referred to authoritative texts on autonomic diseases).
Although they technically belong to the same group of SFN, autonomic neuropathies
are usually studied separately from sensory SFN. Therefore, the term SFN is usually
employed to discuss variants that course with sensory changes (pure or associated
with autonomic findings) and herein we follow this same clinical tradition.
A non-length dependent variant of SFN has recently been reported and will not be discussed
in detail in this review, but the reader is referred to: Brannagan et al.[9 ]; and Khoshnoodi et al.[10 ]. Conditions, like diabetes mellitus, may be associated with forms of SFN associated
with combined sensory and autonomic involvement that seems to be the most prevalent
subtype[11 ]. It is important to highlight, that in addition to unusual clinical symptoms such
as formicationlike phenomena and chronic pruritus[8 ], SFN may be associated with acute neuropathy symptoms (Guillain-Barré-like syndrome),
minimal clinical symptoms (subclinical neuropathy) or other controversial clinical
entities, such as restless leg syndrome[4 ],[11 ].
Neurological examination in patients with small fiber neuropathy
Neurological examination may be normal, usually at the very early onset of the disease,
but eventually pain and thermal sensations will decrease in a length-dependent fashion
in polyneuropathies, following the nerve territories in multiple mononeuropathies,
and in a patchy temperature-dependent fashion in leprosy. A mental status examination
is usually normal, cranial nerve involvement (usually mild and late, except for some
specific variants) may be present for both sensory (involvement of trigeminal sensory
modalities) and autonomic subtypes (pupillary, salivation involvement of IX and X
nerves). Motor examination is entirely normal (including reflexes), although hyporeflexia
also does not invalidate the diagnosis. Sensory examination can, and frequently does,
reveal decreased distal pinprick and temperature sensation (length-dependent fashion).
There is no agreement on whether minor distal vibratory involvement is acceptable
or not. Autonomic abnormalities may be seen on examination, including changes in skin
temperature and color as well as in sweating pattern. Other changes on physical examination
may be particular to different subtypes of SFN, such as genetic forms, leprosy, SFN
associated with systemic diseases and especially with the range of different autonomic
disorders (such as orthostatic hypotension, orthostatic tachycardia). These changes
will not be the subject of this consensus.
Epidemiology of small fiber neuropathy
In the normal population, there is only one study from the Netherlands that reported
an incidence of SFN of 11.7 cases per 100,000 population per year, and higher rates
in individuals older than 65 years[12 ]. The overall prevalence of neuropathy is 2.4% (in those older than 65 years, it
may reach 8%) and estimates of hereditary neuropathy are as high as 1 in 1,214 persons[6 ]. In patients with certain types of systemic conditions, SFN can be highly prevalent,
e.g. celiac and inflammatory bowel diseases[13 ],[14 ],[15 ]. Small fiber neuropathy is a common presentation of axonal idiopathic polyneuropathy
or cryptogenic sensory polyneuropathy. In a cooperative study involving two North
American sites and one South American (Federal Fluminense University in Brazil), cryptogenic
sensory polyneuropathy made up 25% of neuropathy cases[16 ].
Electromyography testing
Nerve conduction parameters can be significantly affected by several physiological
factors, such as body temperature, height, age, anatomical variations and anatomical
differences. Normal values for the different neurophysiological parameters are not
universally accepted. Each neurophysiology laboratory is responsible for setting up
the normal values and usually employs a slightly different profile of common neurophysiological
techniques. In addition, most neurophysiology laboratories do not have adequate normal
values for different age groups. Since most patients undergoing evaluation for peripheral
neuropathy have not had prior electrodiagnostic testing, it is also theoretically
possible that a given neurophysiological parameter, such as sensory or motor amplitude,
may be within the normal range for a certain neurophysiology laboratory but still
reflect an unexplained decrease for a given time interval. Therefore, a normal nerve
conduction study cannot exclude minor large-fiber axonal decrements. This may explain
why a patient labeled with SFN may indeed have a minor decrement of distal vibration.
In this regard, although not necessarily entirely true for all cases, the current
terminology and concept of SFN may serve as an important concept for the diagnosis
of peripheral neuropathy itself and avoid confusion (labeling a patient with symptomatic neuropathy as normal because
of normal EMG results) generated by diverse neurophysiological sets of normal values
within the laboratory tests.
Mechanisms of selective small fiber involvement
Although, most commonly, all types of peripheral axons (motor, sensory and autonomic)
are affected by a specific pathogenic mechanism, different disease processes can lead
to more selective involvement of a particular axon subtype, due to variable reasons.
Diffuse unmyelinated and thinly myelinated fibers can be affected separately in SFN.
The most common site of small fiber involvement is at the distal nerve branches at
the skin, although non-length-dependent involvement has been described. Here, we provide
a growing list of conditions that can lead to selective small fiber involvement in
humans, at least during part of the natural course of the condition ([Table ])[11 ],[13 ],[14 ],[15 ],[17 ],[18 ],[19 ],[20 ],[21 ],[22 ],[23 ],[24 ],[25 ],[26 ],[27 ],[28 ],[29 ],[30 ],[31 ],[32 ]
,333,
[34 ],[35 ],[36 ],[37 ],[38 ],[39 ],[40 ],[41 ],[42 ],[43 ],[44 ].
Table
Causes of small fiber neuropathy.
Metabolic and/or nutritional
Diabetes mellitus, metabolic syndrome/glucose intolerance, insulin neuritis[11 ],[17 ]
Chronic renal insufficiency[18 ]
Thiamine or B12 deficiency (myeloneuropathy spectrum)[11 ],[19 ]
Hyper- or hypothyroidism[11 ],[20 ]
Hyperlipidemia[21 ]
Infectious
Leprosy[22 ]
HIV[23 ], HTLV[24 ]
Hepatitis C[25 ]
Chagas disease[26 ]
Amyloidosis
Systemic[27 ]
Familial (familial amyloid polyneuropathy)[28 ]
Autoimmune
Celiac disease[15 ]
Sjögren's disease[29 ]
Inflammatory bowel disease[13 ],[14 ]
Systemic lupus erythematosus[30 ]
Rheumatoid arthritis[31 ]
Sarcoidosis[32 ]
Guillain-Barré syndrome variants[33 ]
Drugs
Extensive list, chronic metronidazole, statins, anti-TNF inhibitor, bortezomib[11 ],[34 ]
Toxins
Thallium, arsenic, alcohol[35 ],[36 ]
Vaccinations[37 ]
Hereditary forms
Hereditary sensory and autonomic neuropathy, Fabry disease, sodium channelopathies,
Wilson's disease, Pompe disease, Fragile X-associated tremor/ataxia syndrome, Ehlers-Danlos
syndrome
Neurodegenerative
Parkinson's disease, Amyotrophic lateral sclerosis [42 ],[43 ]
Cryptogenic
As we discuss in the next sections, pure (isolated) involvement of small nerve fibers
may be difficult to pinpoint. Most commonly, at least a milder degree of combined
large-fiber involvement is observed, leading us to conclude that, “pure” small fiber
involvement is, in several instances, a fallacy. Selective small fiber involvement
can only be explained by specific genetic conditions and a few auto-immune disorders,
as we will subsequently point out.
Tests to confirm the clinical diagnosis of small fiber neuropathies
By definition, as large fibers are spared in small fiber neuropathies, the most classic
ways to document peripheral nerve involvement yield a totally normal test, i.e. nerve
conduction studies and electromyography are entirely normal in patients with SFN.
Therefore, specialized testing has been developed to evaluate the function of autonomic
and unmyelinated sensory fibers. Although most institutions have developed different
protocols for testing of small fibers, SFN confirmatory tests can grossly be divided
into two subtypes: (A) tests to evaluate somatic (sensory) small fibers; and (B) tests
to evaluate the function of autonomic fibers.
A - Tests for selective evaluation of somatic small fibers
Thermoregulatory sweat test
The thermoregulatory sweat test consists of dusting the body surface with an indicator
powder (alizarin red or starch powder over the body surface with an aqueous iodine
solution – Minor's test). The powder turns from orange to purple or dark purple, to
almost black when wet. The patient is kept in an enclosed cabinet with a moderately
hot and humid environment (45°–50°C air temperature with 35–40% relative humidity).
The mean skin temperature is monitored and kept between 38.5° and 39.5°C (overhead
infrared heaters). The oral temperature must rise at least 1°C or to 38°C (whichever
is higher). Maximal sweating is achieved in 30–65 minutes. Digital photographs of
the sweating and non-sweating areas are taken, and a computerized body image is generated.
A software compares the sweat patterns with laboratory controls. In addition to evaluating
peripheral small fibers, the test also evaluates preganglionic sympathetic fibers
and central nervous system autonomic pathways[45 ]. This test is currently available in a few academic institutions in Brazil.
Quantitative sensory testing
Quantitative sensory tests are psychophysiological tests (dependent on the patient's
self-assessment and report) designed to evaluate the thresholds for detection of vibration,
temperature (most commonly cold, but also warm) sensations and pain. Although they
have been employed in several clinical trials (usually for both small- and large-fiber
involvement), they have significant limitations: central loss cannot be differentiated
from peripheral loss and feigned sensory loss cannot easily be differentiated[46 ]. Newer algorithms decrease the risk of feigned deficits and heat and heat-pain thresholds
are better suited for small fiber assessment. This test is currently available in
a few academic institutions in Brazil. A Brazilian version of a quantitative sensory
testing device has also been validated[47 ].
Contact heat evoked potentials
Contact heat evoked potentials are potentials that are generated by a device that
provides rapid cycles of heat. Their amplitudes correlate with cutaneous nociceptive
nerve fiber density[48 ]. A recent study in patients with Parkinson's disease revealed that contact heat
evoked potential amplitudes could be modulated by dopamine levels (ON and OFF stages),
suggesting that evaluation of small fibers may be affected by central factors when
one uses this technique[49 ] in contrast with a laser evoked potential study[50 ]. Another study concluded that small fibers are affected in patients with meralgia
paresthetica and neuropathic pain[51 ]. There is a strong correlation of contact heat evoked potential findings with the
intra-epidermal nerve fiber density observed in skin biopsy. A recent study reported
normative data for contact heat evoked potentials[52 ]. This study involved several different international peripheral nerve centers including
one in Brazil (Federal Fluminense University, Rio de Janeiro). This method has the
advantage of being noninvasive and does not induce burning lesions in the area where
heat stimuli are applied, contrasting with the laser evoked potentials test. This
test is currently available in very few institutions in Brazil.
B - Tests for selective evaluation of autonomic small fibers
Quantitative sudomotor axonal reflex testing
Quantitative sudomotor axon reflex testing evaluates postganglionic sudomotor function
through iontophoresis induced with acetylcholine applied to the skin. Several complex
devices have been developed to accomplish this task, including a recently-designed
commercially-available machine, called QSweat[53 ]. In general, four sites are mapped, two distal and two proximal in the upper and
lower extremities, allowing the evaluation of a distal-to-proximal neuropathy gradient
involvement. Some authors have observed moderate test-retest reliability (at least
for older versions of the quantitative sudomotor axon reflex test), suggesting limitations
for longitudinal assessment of sudomotor function with quantitative sudomotor axon
reflex testing[54 ]. This test is currently available in very few academic institutions in Brazil.
Skin wrinkling test
Skin wrinkling is mediated by sympathetic activity. The observation that amputated
fingers maintain the ability to vasodilate while not wrinkling supports this premise.
Recent studies have validated osmotic and EMLA-induced skin wrinkling[55 ],[56 ]. They revealed that an index of skin wrinkling from four fingers (all but the thumb)
correlated with the fiber density in skin biopsies[57 ]. The great advantage is the low cost but the evaluation of skin wrinkling may be
limited in hands with calluses or other labor-related issues. Although not very popular
worldwide (despite the low cost), this test has been used in at least one institution
(only for research purposes) in Brazil.
Sympathetic skin responses
Sympathetic skin responses are electrical potentials obtained in the skin by electrodes
that are positioned on the palms and soles of the feet. Potentials are dependent on
the changes in sudomotor activity, providing a measurement of sympathetic cholinergic
activity[58 ]. Different stereotyped stimuli have been used to elicit sympathetic skin responses,
such as inspiratory gasp, auditory stimuli (clapping of the hands) or electrical stimuli.
The sympathetic skin response is mediated through long spinal cord descending pathways
and is not affected by pure autonomic failure restricted to sympathetic adrenergic
pathways. It can be absent in normal old individuals and, therefore, usually is not
considered a very reliable test. However, since it can readily be performed with most
electromyography equipment, it has been used as a screening tool for autonomic involvement
in several centers. This test is probably the most-employed screening tool for autonomic
involvement in Brazil.
Heart rate response to deep breathing and Valsalva test (cardiovagal function tests)
Due to their availability in several electromyography packages worldwide, the heart
rate response to deep breathing and Valsalva ratio tests have frequently been used
in several neurophysiology laboratories. The heart rate response to deep breathing
is affected by several factors (hypocapnia, sympathetic activity, position of the
individual, medication, obesity and depth of breathing) but age and rate of breathing
are the two most important modulating factors. The R-R variation is maximal at rates
of five to six breaths per minute, so the patient is instructed to establish smooth
breathing at this rate (usually following an oscillating bar) in 10-second intervals.
Eight cycles are recorded, followed by a five-minute rest and repeated testing. The
five largest consecutive responses are read, averaged and the heart rate range derived[59 ]. The Valsalva ratio is established by asking the patient to maintain a column of
mercury at 40 mmHg for 15 seconds via a bugle with an air leak, to keep an open glottis.
The Valsalva ratio is derived from the maximum heart rate generated by the Valsalva
maneuver divided by the lowest heart rate within the 30-second period of peak heart
rate[60 ]. In addition to diagnostic purposes, at least for diabetic neuropathies, these tests
can have a prognostic value[57 ]. Although very popular worldwide (due to the low cost), these tests are only used
for research purposes in Brazil (they were not approved for standard routine medical
testing).
C - Tests for evaluation of somatic and/or autonomic small fibers
Skin biopsy
Skin biopsies are increasingly being employed to evaluate small fiber function and
this is the most important diagnostic test to evaluate small fibers in the USA and
Europe (it is starting to become available in Brazil but with level A recommendation
on European guidelines). Standard 3-mm dermatologic punch biopsies can be taken from
any part of the body, but have been standardized for the distal lateral leg and thigh,
to demonstrate a length-dependent pattern[58 ]. The morphometric evaluation of linear density of intra-epidermal nerve fibers should
include normative values for different genders and ages, and this has been considered
a very safe procedure with no serious side effects in 35,000 biopsies over 10 years[61 ]. New technical variants have evolved to evaluate autonomic function (sweat gland
innervation) as well as PMP22 expression and alpha-synuclein deposits[62 ],[63 ]. Evaluation of older patients is less reliable due to higher dispersion/non-Gaussian
distribution. Neuropathy could also be feigned by prior administration of agents that
lead to denervation, such as capsaicin. Another drawback is that decreased intra-epidermal
fiber density could be secondary to involvement of the distal portions of large sensory
fibers, and not necessarily selective involvement of unmyelinated and thinly myelinated
fibers, making the interpretation of the results of the skin biopsies more complicated.
Although skin biopsy is considered a gold-standard test for the diagnosis of small
fiber neuropathies in several recommendations from different societies (e.g. Lauria
et al. 2010[61 ]), an unequivocal clinical-histological correlation is still under discussion. It
is currently available at very few academic institutions in Brazil and has been used
to evaluate HIV-related neuropathy in a Brazilian study[64 ].
In vivo confocal microscopy
The use of confocal microscopy has recently gained attention in the medical literature
to evaluate small fiber function. The cornea is densely innervated by sensory and
autonomic trigeminal fibers (C and Aδ fibers) and corneal confocal microscopy has
also emerged as a new diagnostic tool to evaluate small fiber neuropathy in several
medical conditions. In patients with diabetes mellitus, several parameters of corneal
confocal microscopy correlate with intra-epidermal nerve fiber density[65 ]. Very recently, normative data for corneal confocal microscopy from Brazil (Federal
Fluminense University, Rio de Janeiro) have been reported[66 ].
Grading scale of certainty for the diagnosis of small fiber neuropathies
There have been several attempts to develop scales for the diagnosis of SFN. A recent
tool was designed that proposed to grade SFN according to clinical symptoms, signs
and findings from validated examinations[67 ],[68 ]. A diagnosis of ‘possible’ SFN is present for length-dependent symptoms and/or clinical
signs of small-fiber damage. A diagnosis of ‘probable’ SFN is made by length-dependent
symptoms, clinical signs of small-fiber damage, and normal sural nerve conduction
studies. Finally, a diagnosis of ‘definite’ SFN is made by length-dependent symptoms,
clinical signs of small-fiber damage, normal sural nerve conduction studies, altered
intra-epidermal nerve fiber density at the ankle and/or abnormal quantitative sensory
testing thermal thresholds at the foot. These criteria have been proposed for diabetic
SFN, but can be applied to all patients with suspected SFN, irrespective of the associated
disease or condition. [Figure 1 ] details a flowchart that we have designed for the evaluation of patients with suspected
SFN.
Figure Schematic view of the evaluation of the patient with small fiber neuropathy (SFN)
leading to disease classification as possible, probable and defined according to different
test results. The lower part of the diagram depicts the most common forms of SFN.
Tests to confirm the etiology of small fiber neuropathies
Whenever possible, investigation and testing should be based on disease history, clinical
examination, past medical and family history. There is no evidence-based literature
to establish which tests should initially be employed to evaluate the etiology of
SFN. If no clue is provided by the clinical history, a reasonable (low cost) initial
screening panel should include at least: fasting glucose (or, ideally, a glucose tolerance
test and HgA1c), VDRL, TSH and serum vitamin B12 levels. Testing for total cholesterol
levels, LDL, and triglycerides may also be relevant but are still controversial. A
second step should include homocysteine and methylmalonic acid levels, rheumatoid
factor, immunofixation electrophoresis, ANA, ACE levels, anti-endomysial antibodies
(or antitransglutaminase), HIV, HTLV and hepatitis B and C screening. Additional tests
should be chosen according to gender, nutritional status and age. Lumbar puncture
should be considered whenever an inflammatory, autoimmune or paraneoplastic disorder
is suspected. In Brazil, due to the relatively high prevalence of leprosy in several
regions, leprosy screening (or at least clinical consideration) in patients with SFN
is mandatory. Also, it is important to consider the diagnosis of familial amyloid
neuropathy, a condition that has been detected in some areas in Brazil.
