Effects of autologous platelet rich plasma gel and calcium phosphate biomaterials on bone healing in an ulnar ostectomy model in dogs

M. Rabillard; J.-G. Grand; E. Dalibert; B. Fellah; O. Gauthier; G.W. Niebauer

doi:10.3415/VCOT-09-04-0048

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2009; 22(06): 460-466
DOI: 10.3415/VCOT-09-04-0048

Original Research

Schattauer GmbH

Effects of autologous platelet rich plasma gel and calcium phosphate biomaterials on bone healing in an ulnar ostectomy model in dogs

M. Rabillard

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

,

J.-G. Grand

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

,

E. Dalibert

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

,

B. Fellah

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

²LIOAD INSERM Unit 791, University of Nantes, France

,

O. Gauthier

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

²LIOAD INSERM Unit 791, University of Nantes, France

,

G.W. Niebauer

¹Department of Small Animal Surgery, Veterinary School of Nantes, Atlanpole – La Chantrerie, Nantes Cedex 3, France

› Author Affiliations

Abstract

Summary

The aim of the study was to evaluate the bone healing properties of an osteopromotive platelet rich plasma (PRP) gel in combination with osteoconductive calcium phosphate (CaP) ceramic granules in a long-bone critical size defect in dogs. A standardised 2 cm long ulnar ostectomy was performed bilaterally in four dogs to compare new-bone formation by CaP matrix with and without association with PRP. Radiographic and histological evaluations were performed blindly. Radiographic evaluation was performed at three, six, nine, 12 and 16 weeks postoperatively. Quantitative measurements of new-bone formation were compared using statistical analysis. At explantation 16 weeks after surgery, no significant ossification was present, neither with CaP granules alone nor in association with PRP gel, and there was no difference of radiodensity between the groups. Qualitative histological evaluation demonstrated for both types of implants the presence of non-mineralised fibrous connective tissue around the CaP granules. New-bone formation was only present to a very small extent within the macropores of the CaP granules at the distal boneimplant interface. In our model which exhibited very limited osteoconduction, neither the CaP granules alone nor in association with PRP were sufficient to stimulate bone healing. In this canine model employing a critical size ulnar gap, the combination of CaP granules and PRP did not effectively promote bone regeneration.

Keywords

Calcium phosphate bone substitute - platelet-rich plasma - bone healing - critical-size defect - dog

Full Text

References

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