Key-words:
Bamboo leaf sign - cervical - subependymoma - subpial
Introduction
Subependymoma constitutes about 1%–2% of spinal ependymal tumors.[[1]],[[2]] Even though they may occur anywhere along the spinal cord, C1–C2 is the most frequent
location (24%).[[2]],[[3]],[[4]],[[5]]
Subependymoma often mimic aggressive intramedullary tumors on radiology and frequently
present with pain, sensory-motor deficits, bowel, and bladder dysfunction like them.
Due to their benign biological behavior, complete surgical excision is usually considered
curative. Owing to a highly controversial role of adjuvant therapy, no consensus has
been reached on “optimal” management of these cases.
We report a case of eccentric subpial subependymoma discussing important differentiating
radiological features and surgical nuances with an attempt to define “optimal” management
strategy.
Case Report
A 36-year-old male presented with pain and progressive paresthesia in right-sided
limbs for 3 years without sphincter dysfunction. Examination revealed spastic weakness
(Medical Research Council Grade 4/5) in both upper limbs and right lower limb with
modified McCormick Grade 2 disability. He also had 30%–40% sensory loss to touch and
pain below C5 with impaired posterior column sensations. Magnetic resonance imaging
(MRI) showed a well-defined mass lesion extending from cervicomedullary junction to
C5, causing expansion of the cord without syrinx formation or tumor cysts. Tumor was
eccentrically placed, anterolaterally on right side pushing the spinal cord toward
left. It was T1 hypo to isointense, T2 hyperintense without significant contrast enhancement
[[Figure 1]]. Common intramedullary lesions such as astrocytoma and ependymoma were considered
among the differential diagnoses.
Figure 1: The typical radiological findings in spinal subependymoma. A well-defined eccentrically
placed, intramedullary lesion can be seen extending from cervicomedullary junction
to C5 causing distinctive steep dilation of spinal cord: Bamboo leaf sign. Lesion
is iso to hypointense on T1 -weighted images (a and b) and hyperintense on T2-weighted
image. There is no evidence of perilesional edema or syrinx formation (c). No significant
enhancement is seen on T1-weighted postcontrast image (d and e)
C1–C5 laminectomy for tumor excision was done. Intraoperatively, tumor was greyish,
soft, moderately vascular lesion extending along subpial plane on the right side,
without distinct planes at either end. Upper half of the tumor was found anterolateral
to the cord on right side, and lower part was seen extending ventral to the cord.
No myelotomy was required for tumor excision.
Histopathologically, tumor cells with mildly enlarged anisomorphic nuclei were seen
clustered in the acellular fibrillary matrix. Mitotic activity and necrosis were absent.
These features were consistent with subependymoma. Immunohistochemistry showed weak
tumor cell positivity for glial fibrillary acidic protein (GFAP) and S-100, and negative
for neuron-specific enolase and epithelial membrane antigen (EMA) with low Ki-67 index
(1%) [[Figure 2]].
Figure 2: (a) Microphotograph showing tumor composed of loose aggregates of cells with intervening
hypocellular fibrillary matrix (H and E, *10), (b) Immunohistochemistry showing expression
of glial fibrillary acidic protein (IHC, *20), (c) Immunohistochemistry shows expression
of S-100 (IHC, x20), and (d) Ki-67 proliferation index showing nuclear positivity
in <1% cells (IHC, x20)
Postoperatively, motor power in all four limbs worsened by one grade compared to the
baseline. We planned for radiological follow-up without any adjuvant therapy. At 2-month
follow-up, motor power and spasticity improved with functional recovery.
Discussion
Nearly 50% of subependymoma are identified incidentally. On the other hand, symptomatic
cases occur frequently in 5th–6th decade and rarely affect children.[[6]],[[7]],[[8]] Symptoms may exist months to several years before diagnosis are made, reflecting
their indolent behavior. However, they may mimic more aggressive intramedullary tumors
clinically. Pain and sensory deficits are the most common initial presentation with
the risk of compressive myelopathy and loss of sphincter control later. Therefore,
early surgery may preclude a significant morbidity.
Histopathogenesis of subependymoma still remains elusive, with few authors even reporting
them as a variant of ependymoma based on electron microscopic study.[[9]] Whereas, others believe them to be a separate entity arising from various cells
such as subependymal glial cells, subependymal cell plate or as a result of some developmental
defect.[[10]],[[11]],[[12]] Krishnan et al. proposed their origin from subpial white matter progenitor cells,
which later descend to eccentric, subpial location.[[3]]
In contrast with ependymoma, features such as mitotic activity, ependymal rosettes,
or perivascular pseudorosettes are rarely found. They exhibit the GFAP and S-100 positivity
similar to astrocytic tumors but may show dot-like pattern for EMA due to poor formation
of ependymal-type rosettes.[[6]] Mitotic index is frequently low (<1%).
Radiologically, it is difficult to establish a definitive diagnosis due to lack of
characteristic findings and a limited number of reported cases. On MRI, our case showed
an eccentrically placed tumor causing distinctive steep dilation of the cervical cord.
It was T1 isointense, T2 hyperintense, nonenhancing lesion without peritumoral edema.
Such dilation occurs as a result of tumor growth in the subpial plane and has been
termed “Bamboo leaf sign.” It may help to differentiate them from ependymoma or astrocytoma
which cause gradual fusiform enlargement of the cord.[[13]] Therefore, a high index of suspicion for “subependymoma” should be considered in
“ependymoma” which have little or no edema with minimal or no contrast enhancement.
However, tumor cysts and syringomyelia are very rarely associated with them.
A gross total resection is considered curative without requiring adjuvant therapy.
Intraoperative features such as lobulated shape, minimal vascularity, eccentric subpial
location, and distinct anatomical planes from normal cord facilitate the dissection.
Sometimes, total excision may still be difficult to achieve due to local infiltration
leading to neurological deficits. Therefore, it becomes highly pertinent to define
an “optimal” treatment in managing them.
In our case, we were unable to achieve total excision for the following two reasons.
First, there was loss of plane between tumor and cord parenchyma at cervicomedullary
junction. Second, tumor was extending ventral to the cord at its lower end with the
high risk of cord traction on attempting its removal. A minimal part of tumor was
left behind at these locations to prevent worsening or development of new deficits.
Therefore, such a safe surgical approach may be considered “optimal.”
Transient weakness in immediate postoperative period similar to our case has been
reported earlier in nearly 60% of patients despite a “safe” surgical course; nonetheless,
most (76%) of them improve with time.[[14]] A study also suggests high incidence of poor outcome owing to the cervicothoracic
location of tumor, poor intramedullary microcirculation, and postoperative kyphotic
deformity.[[15]]
Surgical excision forms the cornerstone of management in cervical subependymoma and
also preferred by most surgeons over irradiation in case of recurrence/regrowth of
tumor. Currently, the role of adjuvant radiotherapy remains controversial. Although
more experience and studies with longer follow-up would be required to gain further
evidence.
Conclusion
Cervical subependymoma is an uncommon, benign tumor which lacks characteristic clinicoradiological
findings, and often mimic, frequently occurring aggressive tumors. Complete tumor
excision, though desirable, is not feasible always. Maximal safe resection holds the
key to good postoperative outcome with the limited role of adjuvant therapy.
Declaration of patient consent
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understand that their names and initials will not be published and due efforts will
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