Key-words:
Brain lesion - complication - histopathology - radiology - stereotactic biopsy
Introduction
Use of stereotaxis in neurosurgery first began in the 1950s.[[1]],[[2]] Stereotactic biopsy (STB) is a common technique used to diagnose cerebral lesions.
Histopathological diagnosis is of the key value for determining the treatment modalities
in neuro-oncology. Although the development of cerebral imaging techniques has provided
convenience in determining the nature of the tumor, these imaging techniques have
not replaced the histopathological diagnosis yet. Massive excision with open surgery
is the optimum method that directly affects the survival in neuro-oncology. On the
other hand, the STB method is used as the first choice in the histological diagnosis
of the deeply located cerebral lesions, also in multifocal tumors and lesions located
in functional areas.
The STB procedure is a reliable method for diagnosing the lesions that cannot be excised
through open surgery due to their depth, number, and/or location.
In this study, the data of 84 STB samples of 83 selected patients were presented.
Materials and Methods
All procedures performed in studies involving human participants were in accordance
with the ethical standards of the Institutional and/or National Research Committee
and with the 1964 Helsinki declaration and its later amendments or comparable ethical
standards. Informed consent was obtained from all individual participants included
in the study. The institutional approval of the study and publication was obtained
before the study.
From January 2011 to December 2015, in our department, 83 patients were taken 84 STB
procedures (one patient underwent the procedure twice) to diagnose cerebral lesions
they had according to the magnetic resonance imaging (MRI) results. The demographic
data revealed that, of the 83 patients included in this study, 52 were males and 31
were females with the age ranged from 10 to 83 (mean = 53.6). The STB technique was
used for those patients with multiple lesions, with deeply located lesions, and with
lesions which could not be removed through open surgery. Radiologically, the lesions
were divided into three groups: (1) deeply located lesions, which were also divided
into three subgroups: (i) callosal and pericallosal lesions, (ii) thalamic and basal
ganglia lesions, and (iii) brain stem lesions; (2) lobar lesions, which were of dimension
rendering excision difficult with open surgery, and (3) multifocal lesions.
Stereotactic biopsy technique
Leksell stereotactic frame was placed on the head of the patients, who were under
local anesthesia, and the contrast-enhanced T1-weighted MR images were obtained. The
section width of the images was 2 mm. For the STB procedure, the intense contrasted
parts of the lesions under contrast-enhanced T1-weighted MR images were targeted.
For the noncontrast enhanced lesions, the center of the hyperintense lesion on T2-weighted
MR images was targeted.
While targeting the intense contrasted or noncontrasted lesions, the x, y, and z coordinates
were calculated using the Cartesian system. The patients were under local anesthesia,
and the biopsies were performed through the drill hole, using the Sedan needle kit.
A minimum of three core biopsies was obtained from this target and its periphery for
each individual case. In addition, for the brainstem lesions, the biopsy samples were
obtained through transfrontal trajectory.
Results
Following the biopsy procedures, histopathological diagnoses were made. The histopathological
results of the STB procedures of 83 cases were as follows: 47 gliomas, 12 lymphomas,
5 infections, 4 metastases, 3 histiocytoses, 2 biopsy-negative cases, 2 nonspecific
lesions, 1 vasculitis, 1 infarct, 1 necrosis, 1 round cell malignant tumor, and 1
neuroepithelial cyst [[Table 1]].
Table 1: Histopathlogy of Stereotactic Biopsies
Pathological examination results of callosal and pericallosal lesions
Glioma: 15, lymphoma: 4, and nonspecific lesion: 1.
Pathological examination results of thalamic and basal ganglia lesions
Glioma: 15, lymphoma: 3, abscess: 1, infarct: 1, and negative biopsy: 1 (one more
STB was performed on this patient).
Pathological examination results of multifocal lesions
Lymphoma: 4, glioma: 4, metastasis: 2, encephalitis: 2, round cell malignant tumor:
1, vasculitis: 1, reactive gliosis: 1, necrosis: 1, histiocytosis: 1, demyelinating
plaques: 1, nonspecific lesion: 1, and negative biopsy: 1.
Pathological examination results of lobar single lesions
Glioma: 9, lymphoma: 1, metastasis: 1, cerebritis: 1, histiocytosis: 2, and toxoplasma:
1.
Pathological examination results of brainstem lesions
Glioma: 4, neuroepithelial cyst: 1, metastatic adenocarcinoma: 1, and infarct: 1.
The radiological distribution of the lesions
According to the classification of the lesion on the basis of their radiological locations,
the numbers of the lesions were as in [[Table 2]]. The number of callosal and pericallosal lesions was 20 [[Table 2]] and [[Figure 1]], that of thalamic and basal ganglia located lesions was 21 [[Table 2]] and [[Figure 2]], that of multifocal lesions was 20 [[Table 2]] and [[Figure 3]], that of lobar lesions was 15 [[Table 2]] and [[Figure 4]], and that of brainstem lesions was 7 [[Table 2]] and [[Figure 5]].
Table 2: Distribution of the Cerebral Lesions on Radiological Areas
Figure 1: (a) Grade 4 glial tumor (glioblastoma multiforme), (b) lymphoma, (c) Grade 1 glial
tumor (subependymal giant cell astrositoma)
Figure 2: (a) Abscess, (b) Grade 2 glial tumor (astrocytoma), (c) infarct, (d) Grade 3 glial
tumor (anaplastic astrocytoma)
Figure 3: (a) Histiocytosis and (b) cerebritis
Figure 4: (a) Small round cell tumor, (b) histiocytosis, (c) metastasis
Figure 5: (a) Grade 4 glial tumor (glioblastoma multiforme), (b) Grade 2 glial tumor (atypical
pilocytic astrocytoma), and (c) neuroepithelial cyst 94
For the four cases, histopathological diagnoses of the lesions could not be made:
two of which had negative biopsy samples – normal brain tissue and two had nonspecific
lesions. Therefore, three of these cases underwent biopsy procedure through stereotactic-guided
awake craniotomy, and for the fourth case, the STB technique was applied again after
the recalculation of the coordinates. As a result of these four cases, the histopathological
diagnoses were clarified. Since the biopsy procedures followed for these four cases
were accepted as unsuccessful practices, the success of obtaining positive STB samples
was found to be 95.2% in the current series.
In the intraoperative and the postoperative period, there occurred some complications
in three patients. One patient had permanent hemihypoesthesia after the procedure.
Another patient had complaints of headache, nausea, and vomiting after the procedure,
and on the control computed tomography (CT) scan, a deeply located intracerebral hematoma
of 2 cm was observed. In third patient, acute respiratory failure occurred when the
third biopsy sample was being obtained during the brainstem biopsy procedure. A full
recovery of the respiratory function was observed at the moment of the procedure was
halted. Thus, the complication rate was calculated as 3.6%.
Postoperative control computed tomography scan
Routine control CT scan was not performed on the patients postoperatively. However,
control CT was performed on patients who had the side sign, headache, nausea, and
vomiting on the neurological examination following the procedure. On the control CT
scans, a deeply located cerebral hematoma of 2 cm dimension was determined in one
patient; however, it did not require surgery.
Discussion
Gross-total excision, which is crucially important for survival in the case of glial
tumors, is the surgical approach we prefer to use with glial tumor cases in our clinic.
The extent of tumor resection in gliomas has a significant role on the survival.[[3]],[[4]] In this series, STB was preferred particularly for deeply located tumors, multifocal
lesions, and lobar lesions, which were of dimension rendering excision difficult with
open surgery. STB was not performed on the cases, of which the lesion was localized
at functional areas (motor cortex, Broca's) as the excisions were performed with craniotomy
with the patient awake.
The four patients, who had had no primary diagnosis of any systemic cancer but having
a cerebral mass at the first admission to our clinic, were diagnosed with cerebral
metastasis, and they were referred to the medical oncology department for primary
focus scans and oncological treatment.
The success of obtaining positive STB samples in the current series was 95.2%, whereas
for the previous studies using the Leksell system, it was reported to be over 90%.[[3]],[[5]] Of the four patients with negative biopsy and nonspecific lesions (2 negative and
2 nonspecific), the histopathological diagnoses changed with open surgical excision
in three and repeated STB in one patient.
The complication rate in this series was 3.6% and the previous studies in the literature
reported to have a complication rate from 2% to 5%.[[5]],[[6]],[[7]],[[8]],[[9]],[[10]],[[11]],[[12]],[[13]],[[14]],[[15]],[[16]],[[17]] Except for three patients who developed morbidity after the procedure, no major
morbidity or mortality was detected. For the case who had complaints of headache,
nausea, and vomiting after the procedure, the control CT scans showed a deeply located
intracerebral hematoma of 2 cm. Following the biopsy procedures, no postoperative
control CT scan was performed on any of the patients in this study. Had a routine
CT scan been performed, the number of clinically insignificant small intracerebral
hematomas would have probably been higher.
In their study, Yamada et al. found the mean rate of minor bleeding as 9.9% in their
biopsy series, in which they performed postoperative CT scan on all patients.[[16]] In our study, biopsy was not performed on cases with a high probability of bleeding
and for lesions rich with vascular tissue. Again, open biopsy samples were obtained
from the masses that were localized close to tissues rich in vasculature, and as a
result, the probability of intracerebral hematoma was reduced. When determining the
entry points on the scalp and the targets, routes that were at a distant from the
vascular structures in contrast-enhanced MR images were used. The biopsy samples of
two pediatric patients (aged 10 and 15) with lesions in the brainstem were obtained
with the Leksell frame kit. No complications occurred following the biopsy procedures.
In their pediatric STB series with 62 patients, Pattisapu et al.[[18]] reported the complication rate as 2%.
Stereotactic brainstem biopsy technique can be performed either through transfrontal
or transcerebellar approach. Compared to transcerebellar approach, transfrontal approach
is easier to apply since the patient is positioned supine.[[19]] On the other hand, though the transcerebellar route traverses a much shorter distance,
this approach, which is performed with the help of suboccipital entry point, could
not be very comfortable both for the patient and the neurosurgeon as it is performed
in prone or semi-sitting position. While the transfrontal approach is particularly
used for the upper brainstem lesions, for the brainstem lesions laterally located,
the transcerebellar approach could be the preferred route. The transfrontal approach
has been more often to be reported as the preferred approach for STB, whereas the
complication rates of both approaches have been reported to be similar in the relevant
literature.[[20]]
According to the histopathological examination, 47 (56.6%) of 83 cases were diagnosed
with glial tumors in this series. Eight of the glial tumors were low grade. Eight
cases were those on which gross excision could not be performed owing to the location
of the lesions, which posed a surgical excision risk. The applicability of STB in
low-grade glial tumors is limited due to the necessity of radical resection in those
cases to achieve long survival.
Twelve (14.4%) of the lesions were diagnosed as lymphomas, and in terms of radiological
distribution, lymphoma was seen everywhere except for the brainstem. Lymphomas are
generally seen as a solitary lesion in the white matter, mostly in the basal ganglia
and the corpus callosum.[[21]] In the current study, lymphomas were notable as the most common multifocal lesions
according to the radiological distribution. However, the review of the literature
did not yield sufficient results except for a few cases for the lymphomas located
in the brainstem.[[22]],[[23]],[[24]],[[25]] Future studies on brainstem and lymphoma if possible with more cases could add
to the relevant literature and foster new approaches toward the treatment.
Conclusion
The success of obtaining positive STB samples in the current series was 95.2%. The
complication rate in this series was 3.6%. Had a routine CT scan been performed on
each patient in this series, the number of clinically insignificant small intracerebral
hematomas would have probably been higher. Nevertheless, the accuracy of the STB sampling
rate and the complication rate were similar to those reported in the relevant literature.
STB is still a highly safe method in diagnosing lesions that are of dimensions that
render them impossible to be excised by surgery and that are high in number or deeply
located.
