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Thromb Haemost 1968; 19(03/04): 397-402
DOI: 10.1055/s-0038-1651218
Originalarbeiten – Original Articles – Travaux Originaux
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Formation of Anticoagulants by Digesting Fibrin with Thrombin[*]

D. C Triantaphyllopoulos
1   Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
,
C. R Muirhead
1   Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
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Publication Date:
27 June 2018 (online)

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Summary

Purified thrombin was able to digest fibrin clots in the combined presence of ε-ACA and Trasylol. The digestion products prolonged the thrombin clotting time of both fibrinogen and plasma and inhibited completely the coagulation of shed blood. The incidental observation was made that phosphate buffer elutes from Amberlite IRC-50 a nondialyzable anticoagulant which can easily be neutralized by calcium salts.

* Supported by Grants HE 7144-03 and HE 11090-01 of the U. S. Public Health Service.

* Presented at the 10th Annual Meeting of the Canadian Federation of Biological Societies, Montreal, 1967.


 

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