The Effect of Copper Ions on Clot Lysis Time in the Fibrinolytic Assay of Streptokinase

Summary

Experiments were conducted to determine why different lots of Borate Buffer reagent affect the clot lysis times obtained in the fibrinolytic assay of Streptokinase. Minerals naturally occurring in distilled water were screened individually to determine their influence on lysis. Copper was found to have a very pronounced effect in this regard on the fibrinolytic system in that low levels reduce the lysis time and high levels increase it.

• References

• 1 Christensen L. R. Methods for Measuring the Activity of Components of the Streptococcal Fibrinolytic System, and Streptococcal Dexoxyribonuclease. J. clin. Invest. 28: 163-172 1949;
• 2 Bogert A. B, Corbett J. J. The Quantitative Effects of Cations on Erythrocyte Agglutination by Influenza Virus V acci ne. J. Immunol. 81: 524-526 1958;
• 3 Ronwin E. Enzymatic Properties of Bovine Plasmin Preparations: Evidence for Similarity to, but Non-identity with Trypsin. Canad. J. Biochem. 34: 1169 1956;
• 4 Ronwin E. Thrombin Properties. Canad. J. Biochem. 35: 743 1957;
• 5 Kowalski et al. Influence of Metallic Ions on Plasmin. Sang 27:No. 5 1956