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Exp Clin Endocrinol Diabetes 2000; Vol. 108(3): 214-219
DOI: 10.1055/s-2000-7745
Articles

© Johann Ambrosius Barth

Effect of four cGMP analogues with different mechanisms of action on hormone release by porcine ovarian granulosa cells in vitro

A. V. Sirotkin 1 , A. V. Makarevich 1 , H.-G. Genieser 2 , J. Kotwica 3 , L. Hetényi 1
  • 1 Research Institute of Animal Production, 94992 Nitra, Slovakia
  • 2 BIOLOG Life Science Institute, 28071 Bremen, Germany
  • 3 Institute of Animal Production and Food Research, 10-718 Olsztyn-Kortowo, Poland
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Publication History

Publication Date:
31 December 2000 (online)

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Summary:

The aim of our in-vitro experiments was to examine the role of cGMP-dependent intracellular mechanisms in control of ovarian hormone secretion, as well as to understand, whether cGMP effect on the ovary may be mediated by either protein kinase G (PKG), cGMP-gated ion channels (CGI) or cGMP-specific phosphodiesterases (PDE).

We compared the effects of the cGMP analogues 8-pCPT-cGMP, an activator of PKG 1-alpha, 1-beta and type II and of CGI, but not of PDE; Rp-8-pCPT-cGMPS and Rp-8-Br-cGMPS, inhibitors of PKG, stimulators of CGI with no effect of PDE, and Rp-8-Br-PET-cGMPS, an inhibitor of both, PKG and CGI and stimulator of PDE (all at 0.01, 0.1, 1, 10 or 100 nM), on the release of oxytocin (OT) and progesterone (P) by cultured porcine granulosa cells.

It was observed, that Rp-8-pCPT-cGMPS significantly (p < 0.05) suppressed OT release when given at 1 or 10 nM. Rp-8-Br-cGMPS increased OT output, when given at 1-10 nM too, but decreased it at 100 nM. Rp-8-Br-PET-cGMPS inhibited OT release at 1 nM. No influence of 8-pCPT-cGMP on OT output was found. 8-pCPT-cGMP stimulated P release at 0.1, 10 or 100 nM. All other cGMP analogues studied suppressed P release at all doses used.

The present observations suggest the involvement of cGMP-dependent intracellular mechanisms in control of ovarian steroid and nonapeptide hormone release. The lack of association between patterns of influence of cGMP analogues on CGI and PDE, and the coincidence of the majority of effects of cGMP analogues on P, OT and PKG may indirectly indicate that cGMP action on release of ovarian hormones is mediated mainly by PKG, but not by CGI or PDE.

Key words:

Oxytocin - progesterone - protein kinase G - cGMP-gated ion channels - phosphodiesterase

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1 + - stimulation, - - inhibition, ± - biphasic effect, 0 - no effect

Dr. Alexander V. Sirotkin

Head, Lab. Endocrinology

Research Institute of Animal Production

Hlohovská 2

SK-94992 Nitra

Slovak Republic

Phone: +4 21-87-54 63 35

Fax: +4 21-87-54 63 61

Email: sirotkin@vuzv.sk