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Horm Metab Res 2003; 35(4): 222-227
DOI: 10.1055/s-2003-39478
Original Basic
© Georg Thieme Verlag Stuttgart · New York

Suppression of Aquaporin Adipose Gene Expression by Isoproterenol, TNFα, and Dexamethasone

M.  Fasshauer 1 , J.  Klein 2 , U.  Lossner 1 , M.  Klier 1 , S.  Kralisch 1 , R.  Paschke 1
  • 1 University of Leipzig, Department of Internal Medicine III, Leipzig, Germany
  • 2 University of Lübeck, Department of Internal Medicine I, Lübeck, Germany
Further Information

Publication History

Received 4 June 2002

Accepted after Revision 19 December 2002

Publication Date:
02 June 2003 (online)

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Abstract

Aquaporin adipose (AQPap) is a putative glycerol channel in adipocytes. It has recently been shown to be upregulated in insulin resistance stimulated by thiazolidinediones and inhibited by insulin. To further clarify regulation of AQPap gene expression, 3T3-L1 adipocytes were chronically treated with various hormones known to influence insulin sensitivity and adipocyte metabolism, and AQPap mRNA was measured by quantitative real-time reverse transcription-polymerase chain reaction. Interestingly, treatment of 3T3-Ll adipocytes with 10 µM isoproterenol, 10 ng/ml TNFα, and 100 nM dexamethasone for 16 h inhibited AQPap gene expression by 62 %, 60 %, and 39 %, respectively; angiotensin 2, growth hormone, and triiodothyronine did not have any effect. The inhibitory effects were dose-dependent with significant suppression detectable at concentrations as low as 1 nM isoproterenol, 1 ng/ml TNFα, and 10 nM dexamethasone. Furthermore, inhibition of AQPap gene expression could be almost completely reversed by pretreating 3T3-L1 adipocytes with the β-adrenoceptor antagonist propranolol. Moreover, stimulation of Gs-proteins with cholera toxin and adenylyl cyclase with forskolin and dibutyryl-cAMP dramatically downregulated AQPap mRNA. Taken together, our results suggest that AQPap is an adipocyte-expressed glycerol channel selectively regulated and profoundly downregulated by hormones implicated in the pathogenesis of insulin resistance and dyslipidemia.

Key words

3T3-L1 adipocyte - AQPap - Glycerol channel - Insulin resistance - Obesity

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Prof. Dr. R. Paschke

Ph.-Rosenthal-Str.27 · 04103 Leipzig · Germany ·

Phone: +49 (34) 9713200

Fax: +49 (341) 9713209

Email: pasr@medizin.uni-leipzig.de