Download PDF
Exp Clin Endocrinol Diabetes 2004; 112(8): 440-443
DOI: 10.1055/s-2004-821190
Article

J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

No Association of IL-1β C + 3954 T Polymorphism and Thyroid Disease

I. Reichmann1 , K. M. Schulte1 , H. D. Roeher1
  • 1Department of General and Visceral Surgery, Heinrich-Heine-University, Düsseldorf, Germany
Further Information

Publication History

Publication Date:
16 September 2004 (online)

Also available at
    Permissions and Reprints

Abstract

Interleukin-1 is a potent pro inflammatory agent, has a direct influence on human thyroid cell function, and modulates cell growth in differentiated thyroid carcinoma cell lines. To evaluate whether a polymorphism on the IL-1β gene has an influence on the incidence of thyroid disorders, we analyzed the C + 3954 T polymorphism in DNA samples of 673 individuals. 414 venous blood samples were collected from patients suffering from thyroid diseases (Graves' disease n = 53, euthyroid or hyperthyroid non-immunogenic benign thyroid disorders n = 240, thyroid carcinoma n = 121). 259 persons without thyroid disease served as a control. There was no statistically significant association between either of the thyroid alterations or functional conditions on the one hand and the examined genetic polymorphism on the other. Because of the large number of samples tested we can conclude with a high degree of confidence that there is no association between the genotype and the surveyed diseases.

Key words

C + 3954 T - polymorphism - thyroid - interleukin-1β

References

  • 1 Ashizawa K, Yamashita S, Tobinaga T, Nagayama Y, Kimura H, Hirayu H, Izumi M, Nagataki S. Inhibition of human thyroid peroxidase gene expression by interleukin 1.  Acta Endocrinol (Copenh). 1989;  121 465-469
    PubMedGoogle Scholar
  • 2 Bidwell J, Keen L, Gallagher G, Kimberly R, Huizinga T, McDermott M F, Oksenberg J, McNicholl J, Pociot F, Hardt C, D'Alfonso S. Cytokine gene polymorphism in human disease: on-line databases.  Genes Immun. 1999;  1 3-19
    CrossrefPubMedGoogle Scholar
  • 3 Buchner A, Faul F, Erdfelder E. G·Power. A priori, post-hoc, and compromise power analyses for the Macintosh (Version 2.1.2). (URL: http://www.psycho.uni-duesseldorf.de/aap/projects/gpower/). Düsseldorf, Germany; Heinrich-Heine-Universität 1996
    Google Scholar
  • 4 Cohen J. Statistical power analysis for the behavioral sciences. Revised edition. Hillsdale, NJ; Lawrence Erlbaum Associates Inc. 1977
    Google Scholar
  • 5 Dinarello C A. The biology of interleukin-1.  Chem Immunol. 1992;  51 1-32
    PubMedGoogle Scholar
  • 6 El-Omar E M, Carrington M, Chow W H, McColl K E, Bream J H, Young H A, Herrera J, Lissowska J, Yuan C C, Rothman N, Lanyon G, Martin M, Fraumeni Jr J F, Rabkin C S. Interleukin-1 polymorphisms associated with increased risk of gastric cancer.  Nature. 2000;  404 398-402 Erratum in.  Nature. 2001;  412 99
    CrossrefPubMedGoogle Scholar
  • 7 Erdfelder E, Faul F, Buchner A. GPower: A general power analysis program.  Behavior Research Methods, Instruments, and Computers. 1996;  28 1-11
    CrossrefPubMedGoogle Scholar
  • 8 Gehrke L, Jobling S A, Paik L S, McDonald B, Rosenwasser L J, Auron P E. A point mutation uncouples human interleukin-1 beta biological activity and receptor binding.  J Biol Chem. 1990;  265 5922-5925
    PubMedGoogle Scholar
  • 9 Grubeck-Loebenstein B, Buchan G, Chantry D, Kassal H, Londei M, Pirich K, Barrett K, Turner M, Waldhausl W, Feldmann M. Analysis of intrathyroidal cytokine production in thyroid autoimmune disease: thyroid follicular cells produce interleukin-1 alpha and interleukin-6.  Clin Exp Immunol. 1989;  77 324-330
    PubMedGoogle Scholar
  • 10 Hamajima N, Matsuo K, Saito T, Tajima K, Okuma K, Yamao K, Tominaga S. Interleukin 1 polymorphisms, lifestyle factors, and Helicobacter pylori infection.  Jpn J Cancer Res. 2001;  92 383-389
    PubMedGoogle Scholar
  • 11 Houssiau F, Van Snick J. IL6 and the T-cell response.  Res Immunol. 1992;  143 740-743
    PubMedGoogle Scholar
  • 12 Inokuchi N, Zeki K, Morimoto I, Nakano Y, Fujihira T, Yamashita U, Yanagihara N, Izumi F, Eto S. Stimulatory effect of interleukin-1 alpha on proliferation through a Ca2 + /calmodulin-dependent pathway of a human thyroid carcinoma cell line, NIM 1.  Jpn J Cancer Res. 1995;  86 670-676
    PubMedGoogle Scholar
  • 13 Kamogashira T, Sakaguchi M, Ohmoto Y, Mizuno K, Shimizu R, Nagamura K, Nakai S, Masui Y, Hirai Y. Site-specific mutagenesis of the human interleukin-1 beta gene: the role of arginine residue at the N-terminal region.  J Biochem (Tokyo). 1988;  104 837-840
    PubMedGoogle Scholar
  • 14 Kasai K, Hiraiwa M, Emoto T, Kuroda H, Hattori Y, Mochizuki Y, Nakamura T, Shimoda S. Presence of high affinity receptor for interleukin-1 (IL-1) on cultured porcine thyroid cells.  Horm Metab Res. 1990;  22 75-79
    PubMedGoogle Scholar
  • 15 Kimura H, Yamashita S, Namba H, Tominaga T, Tsuruta M, Yokoyama N, Izumi M, Nagataki S. Interleukin-1 inhibits human thyroid carcinoma cell growth.  J Clin Endocrinol Metab. 1992;  75 596-602
    CrossrefPubMedGoogle Scholar
  • 16 March C J, Mosley B, Larsen A, Cerretti D P, Braedt G, Price V, Gillis S, Henney C S, Kronheim S R, Grabstein K. et al . Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.  Nature. 1985;  315 641-647
    CrossrefPubMedGoogle Scholar
  • 17 Nicklin M J, Weith A, Duff G W. A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes.  Genomics. 1994;  19 382-384
    CrossrefPubMedGoogle Scholar
  • 18 Nilsson M, Husmark J, Bjorkman U, Ericson L E. Cytokines and thyroid epithelial integrity: interleukin-1 alpha induces dissociation of the junctional complex and paracellular leakage in filter-cultured human thyrocytes.  J Clin Endocrinol Metab. 1998;  83 945-952
    CrossrefPubMedGoogle Scholar
  • 19 Pociot F, Molvig J, Wogensen L, Worsaae H, Nerup J. A TaqI polymorphism in the human interleukin-1 beta (IL-1 beta) gene correlates with IL-1 beta secretion in vitro.  Eur J Clin Invest. 1992;  22 396-402
    PubMedGoogle Scholar
  • 20 Rasmussen A K, Diamant M, Blichert Toft M, Bendtzen K, Feldt Rasmussen U. The effects of interleukin-1 beta (IL-1 beta) on human thyrocyte functions are counteracted by the IL-1 receptor antagonist.  Endocrinology. 1997;  138 2043-2048
    CrossrefPubMedGoogle Scholar
  • 21 Rasmussen A K, Feldt Rasmussen U, Bendtzen K. The effect of interleukin-1 on the thyroid gland.  Autoimmunity. 1993;  16 141-148
    PubMedGoogle Scholar
  • 22 Rasmussen A K, Kayser L, Feldt Rasmussen U, Bendtzen K. Influence of tumour necrosis factor-alpha, tumour necrosis factor-beta and interferon-gamma, separately and added together with interleukin-1 beta, on the function of cultured human thyroid cells.  J Endocrinol. 1994;  143 359-365
    PubMedGoogle Scholar
  • 23 Reimers J I, Rasmussen A K, Karlsen A E, Bjerre U, Liang H, Morin O, Andersen H U, Mandrup Poulsen T, Burger A G, Feldt Rasmussen U, Nerup J. Interleukin-1 beta inhibits rat thyroid cell function in vivo and in vitro by an NO-independent mechanism and induces hypothyroidism and accelerated thyroiditis in diabetes-prone BB rats.  J Endocrinol. 1996;  151 147-157
    CrossrefPubMedGoogle Scholar
  • 24 Sato K, Satoh T, Shizume K, Ozawa M, Han D C, Imamura H, Tsushima T, Demura H, Kanaji Y, Ito Y. et al . Inhibition of 125I organification and thyroid hormone release by interleukin-1, tumor necrosis factor-alpha, and interferon-gamma in human thyrocytes in suspension culture.  J Clin Endocrinol Metab. 1990;  70 1735-1743
    PubMedGoogle Scholar
  • 25 Svenson M, Kayser L, Hansen M B, Rasmussen A K, Bendtzen K. Interleukin-1 receptors on human thyroid cells and on the rat thyroid cell line FRTL-5.  Cytokine. 1991;  3 125-130
    CrossrefPubMedGoogle Scholar
  • 26 Tominaga T, Yamashita S, Nagayama Y, Morita S, Yokoyama N, Izumi M, Nagataki S. Interleukin 6 inhibits human thyroid peroxidase gene expression.  Acta Endocrinol (Copenh). 1991;  124 290-294
    PubMedGoogle Scholar
  • 27 Watson P F, Pickerill A P, Davies R, Weetman A P. Semi-quantitative analysis of interleukin-1 alpha, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes.  J Mol Endocrinol. 1995;  15 11-21
    PubMedGoogle Scholar
  • 28 Weetman A P, Bright Thomas R, Freeman M. Regulation of interleukin-6 release by human thyrocytes.  J Endocrinol. 1990;  127 357-361
    PubMedGoogle Scholar
  • 29 Weetman A P, Cohen S, Makgoba M W, Borysiewicz L K. Expression of an intercellular adhesion molecule, ICAM-1, by human thyroid cells.  J Endocrinol. 1989;  122 185-191
    Google Scholar
  • 30 Yip I, Pang X P, Berg L, Hershman J M. Antitumor actions of interferon-gamma and interleukin-1 beta on human papillary thyroid carcinoma cell lines.  J Clin Endocrinol Metab. 1995;  80 1664-1669
    CrossrefPubMedGoogle Scholar
  • 31 Zeki K, Morimoto I, Arao T, Eto S, Yamashita U. Interleukin-1 alpha regulates G1 cell cycle progression and arrest in thyroid carcinoma cell lines NIM1 and NPA.  J Endocrinol. 1999;  160 67-73
    CrossrefPubMedGoogle Scholar
  • 32 Zeki K, Nakano Y, Inokuchi N, Watanabe K, Morimoto I, Yamashita U, Eto S. Autocrine stimulation of interleukin-1 in the growth of human thyroid carcinoma cell line NIM 1.  J Clin Endocrinol Metab. 1993;  76 127-133
    CrossrefPubMedGoogle Scholar

Ira Reichmann

Department of General and Visceral Surgery
Heinrich-Heine-University

Moorenstraße 5

40225 Düsseldorf

Germany

Phone: + 49(0)2118117376

Fax: + 49 (0) 21 18 11 50 37

Email: Ira.Reichmann@uni-duesseldorf.de