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Vet Comp Orthop Traumatol 2011; 24(01): 39-44
DOI: 10.3415/VCOT-10-05-0070
Original Research
Schattauer GmbH

Evaluation of in vitro serial antibiotic elution from meropenem-impregnated polymethylmethacrylate beads after ethylene oxide gas and autoclave sterilization

L. A. Báez
1   College of Veterinary Medicine, Mississippi State, Mississippi, United States
,
C. Langston
1   College of Veterinary Medicine, Mississippi State, Mississippi, United States
,
S. Givaruangsawat
1   College of Veterinary Medicine, Mississippi State, Mississippi, United States
,
R. McLaughlin
1   College of Veterinary Medicine, Mississippi State, Mississippi, United States
› Author Affiliations
Further Information

Publication History

Received: 07 May 2010

Accepted: 30 July 2010

Publication Date:
19 December 2017 (online)

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Summary

Objectives: To determine the elution properties of meropenem and to compare the elutions of meropenem-impregnated polymethylmethacrylate (PMMA) beads without sterilization (P-M-C) to those sterilized with steam (P-M-A) and ethylene oxide gas (P-M-EO).

Methods: A commercial bead mould was used to produce four groups of beads: one group without antibiotic (negative control), and three groups of meropenem-impregnated beads: P-M-C, P-M-A, and P-M-EO. The beads were placed in a phosphate buffered solution and eluent samples were collected. Concentrations of the antibiotic in eluent samples from the two sterilized groups and the control beads were determined using a microbiological assay at 1, 3, 6 and 12 hours and at 1, 2, 3, 6, 9, 12, 15, 18, 22, 26, and 30 days.

Results: The microbiological assay resulted in no zone of inhibition at all time periods for the P-M-A samples and the samples of PMMA without antimicrobial. The meropenem concentration on the eluent remained above 4 mcg/ml for 15 days in the P-M-C group and until day 18 for P-M-EO group. There was no statistical difference in AUC0-∞ (p <0.318), however significance did occur for MRT (p <0.005) when comparing P-M-C and P-M-EO with the later being higher.

Discussion: The meropenem incorporated in the PMMA beads eluted effectively and gradually decreased after the 24 hour peak, but remained above the concentration level of 4 mcg/ml for 15 days in the P-M-C group and until day 18 for P-M-EO group. Ethylene oxide does not adversely affect meropenem’s elution from PMMA beads.

Keywords

Antibiotic-impregnated polymethylmethacrylate beads - meropenem - PMMA beads - polymethylmethacrylate - sterilization
 

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