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Thromb Haemost 1998; 79(02): 383-388
DOI: 10.1055/s-0037-1614996
Letters to the Editor
Schattauer GmbH

Effect of SR121566A, a Potent GP IIb-IIIa Antagonist on Platelet-mediated Thrombin Generation In Vitro and In Vivo

J. P. Herault
1   From the Haemobiology Research Department, Sanofi Recherche, Toulouse, France
,
V. Peyrou
1   From the Haemobiology Research Department, Sanofi Recherche, Toulouse, France
,
P. Savi
1   From the Haemobiology Research Department, Sanofi Recherche, Toulouse, France
,
A. Bernat
1   From the Haemobiology Research Department, Sanofi Recherche, Toulouse, France
,
J. M. Herbert
1   From the Haemobiology Research Department, Sanofi Recherche, Toulouse, France
› Author Affiliations
Further Information

Publication History

Received 27 August 1997

Accepted 03 October 1997

Publication Date:
08 December 2017 (online)

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Summary

The effect of SR121566A, a new non-peptide GP IIb-IIIa antagonist was studied in vitro with regard to thrombin generation in platelet rich plasma and in vivo on stasis-induced venous thrombosis in the rabbit. SR121566A inhibited ADP-, arachidonic acid- and collagen-induced human platelet aggregation with IC50 values of 46 ± 7.5, 56 ± 6 and 42 ± 3 nM, respectively. In the same experimental conditions, SR121566A strongly inhibited thrombin generation triggered by low concentrations of tissue factor. SR121566A reduced in a dose-dependent manner both the area under the curve and the thrombin peak concentration but did not affect the lag phase (defined as the time until 10 nM thrombin was generated). Aspirin (100 µg/ml) did not affect thrombin generation.

One hour after intravenous administration to rabbits, SR121566A exhibited a potent ex vivo inhibitory effect against ADP-, arachidonic acid- and collagen-induced platelet aggregation. The ID50 were 0.6 ± 0.25, 0.7 ± 0.08 and 0.13 ± 0.08 mg/kg, respectively. The ability of aspirin and SR121566A to affect venous stasis was determined in a stasis-induced venous thrombosis model in rabbits under high and low thrombogenic challenges. While aspirin was ineffective in both conditions, SR121566A significantly inhibited thrombus formation under low thrombogenic challenge demonstrating for the first time that a potent non-peptide platelet GP IIb-IIIa antagonist inhibits thrombin generation in vivo and exhibits a strong antithrombotic effect with regard to stasis-induced venous thrombosis. These results therefore confirm the existence of a close relationship between platelet activation and thrombin generation leading to blood coagulation but also emphasise the key role of platelets in the development of venous thrombosis, most likely through activation of the GP IIb-IIIa complex.

 

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