Heparin-Associated Thrombocytopenia: The Effects of Various Intravenous lgG Preparations on Antibody Mediated Platelet Activation - A Possible New Indication for High Dose i.v. IgG

A Greinacher; U Liebenhoff; V Kiefel; P Presek; C Mueller-Eckhardt

doi:10.1055/s-0038-1642496

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1994; 71(05): 641-645
DOI: 10.1055/s-0038-1642496

Review Article

Schattauer GmbH Stuttgart

Heparin-Associated Thrombocytopenia: The Effects of Various Intravenous lgG Preparations on Antibody Mediated Platelet Activation - A Possible New Indication for High Dose i.v. IgG

A Greinacher

¹The Institute for Clinical Immunology and Transfusion Medicine, Giessen, FRG

,

U Liebenhoff

²Rudolf-Buchheim-Institut für Pharmakologie, Justus Liebig University, Giessen, FRG

,

V Kiefel

¹The Institute for Clinical Immunology and Transfusion Medicine, Giessen, FRG

,

P Presek

²Rudolf-Buchheim-Institut für Pharmakologie, Justus Liebig University, Giessen, FRG

,

C Mueller-Eckhardt

¹The Institute for Clinical Immunology and Transfusion Medicine, Giessen, FRG

› Institutsangaben

Abstract

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Summary

The immunologic type of heparin-associated thrombocytopenia (HAT) is caused by antibodies which activate platelets via the Fc-re- ceptor in the presence of polysulfated oligosaccharides. The antigen is formed by a releasable platelet protein (in many cases PF4) complexed to heparin. Since the role of GP Ilb/IIIa in platelet activation by HAT antibodies is controversial, we investigated platelet activation by antibodies related to HAT. We used normal platelets and platelets from a patient with Glanzmann’s thrombasthenia (GT) lacking GP Ilb/IIIa. Heparin and sera from patients with HAT stimulated GT platelets in the same manner as determined by ¹⁴C-serotonin release and the changes in phosphorylation of p20 and p47. Platelet activation could be inhibited by an anti FcRII monoclonal antibody (IV. 3, Fab-fragments), and by Fc-fragments, but not by F(ab’)₂-fragments of human IgG. The effect of four different, commercially available preparations of intact i.v. IgG on the platelet activation by six HAT sera was investigated by ¹⁴C-seroto- nin release. The inhibitory effect was strongly dependent upon the manufacturing process. At a concentration of 20 mg/ml only IgG that had been subjected to low pH and traces of pepsin sufficiently inhibited platelet activation. IgG treated with polyethylenglycol or sulfitolysis was less effective, whereas beta-propiolactone-treated IgG almost completely lost the ability to inhibit platelet activation by antibodies related to HAT. We conclude that inhibition of GP Ilb/IIIa-fibrinogen interaction is insufficient for preventing platelet activation in HAT. This is, however, possible by high dose i.v. IgG, whereby inhibition of FcRII on platelets strongly depends upon the process by which the i.v. IgG preparation was manufactured.

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Referenzen

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