Download PDF
Thromb Haemost 1996; 76(03): 361-368
DOI: 10.1055/s-0038-1650584
Original Article
Schattauer GmbH Stuttgart

Activation of Factor X by Factor VIIa Complexed with Human-mouse Tissue Factor Chimeras Requires Human Exon 3

Carrie H Fang
1   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT
,
T-C Lin
1   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT
,
Arabinda Guha
2   Th Departments of Medicine and Biochemistry, Mount Sinai School of Medicine, City University of New York, New York, NY, USA
,
Yale Nemerson
2   Th Departments of Medicine and Biochemistry, Mount Sinai School of Medicine, City University of New York, New York, NY, USA
,
William H Konigsberg
1   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT
› Author Affiliations
Further Information

Publication History

Received 03 July 1995

Accepted after resubmission 26 April 1996

Publication Date:
26 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

In an attempt to define sequence elements in human and mouse tissue factor (TF) that are responsible for the species specificity observed in their interaction with human factor VIIa (HVIIa), we constructed human-mouse chimeric TF cDNAs, inserted them into plasmid vectors, and induced their expression in E.coli. Assays for procoagulant activity were carried out with the resulting E. coli lysates using (HVIIa) human and mouse (MVIIa). The ratio of the procoagulant activities, HVIIa/MVIIa, revealed that human TF exon 3 was essential for activity when the TF:VIIa complex was formed with HVIIa. By ligating the maltose binding protein (MBP) gene to TF cDNAs it was possible to construct, express and purify MBP-TF chimeras as well as to estimate their specific activities. With selected MBP-TF chimeras and HVIIa we determined kinetic parameters for the activation of human factor X. Replacement of exon 3 in human TF cDNA with the corresponding exon from mouse TF cDNA resulted in both lower affinity for HVIIa and failure to convert bound HVIIa into a potent protease

 

  • References

  • 1 Nemerson Y. The reaction between bovine tissue factor and factors VII and X. Biochemistry 1966; 5: 601-608
    CrossrefPubMedSearch in Google Scholar
  • 2 Nemerson Y. Tissue factor and hemostasis. Blood 1988; 71: 1-8
    PubMedSearch in Google Scholar
  • 3 Edgington TS, Mackman N, Brand K, Ruf W. The structural of expression and function of tissue factor. Thromb Haemost 1991; 66: 64-79
    PubMedSearch in Google Scholar
  • 4 Carson D, Brozna JP. Role of tissue factor in the production of thrombin. Blood Coag. Fibrinol 1993; 4: 281-292
    PubMedSearch in Google Scholar
  • 5 Osterud B, Rapaport SI. Activation of factor IX by the reaction product of tissue factor and factor VII: Additional pathway for initiating blood coagulation. Proc Natl Acad Sci USA 1977; 74: 5260-5264
    CrossrefPubMedSearch in Google Scholar
  • 6 Bach R. Initiation of coagulation by tissue factor. CRC Critical Reviews in Biochemistry 1988; 23: 339-368
    PubMedSearch in Google Scholar
  • 7 Nemerson Y, Gentry R. An ordered addition, essential activation model of the tissue factor pathway of coagualtion: Evidence for a conformational cage. Biochemistry 1986; 25: 4020-4033
    CrossrefPubMedSearch in Google Scholar
  • 8 Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Am J Pathology 1989; 134: 1087-1097
    PubMedSearch in Google Scholar
  • 9 Cole EH, Schulman J, Urowitz M, Keystone E, Williams C, Levy GA. Monocyte procoagulant activity in glomerulonephritis associated with systemic lupus erythematosus. J Clin Invest 1985; 75: 861-868
    CrossrefPubMedSearch in Google Scholar
  • 10 Halloran P, Aprile M, Haddad G, Roginette M. The significance ofelevated procoagulant activity in the monocytes of renal transplant recipients. Transplant Proc 1982; 14: 669-672
    PubMedSearch in Google Scholar
  • 11 Osterud B, Flaegstad T. Increased tissue thromboplastin activity in monocytes of patients with meningococcal infection: related to an unfavorable prognosis. Thromb Haemost 1983; 49: 5-7
    Thieme ConnectPubMedSearch in Google Scholar
  • 12 Wilcox JN, Smith KM, Schwartz S, Gordon D. Localization of tissue factor in the normal vessel wall and in the artherosclerotic plaque. Proc Natl Acad Sci USA 1989; 86: 2839-2834
    CrossrefPubMedSearch in Google Scholar
  • 13 Silverberg S, Nemerson Y, Zur M. Kinetics of the activation of bovine coagulation factor X by components of the extrinsic pathway. J Biol Chem 1977; 252: 8481-8488
    PubMedSearch in Google Scholar
  • 14 Kadish JL, Wenc KM, Dvorak HF. Tissue factor activity of normal and neoplastic cells: quantitation and species specificity. JNCI 1983; 70: 551-557
    PubMedSearch in Google Scholar
  • 15 Janson TL, Stormoken H, Prydz H. Species Specificity of tissue thromboplastin. Hemostasis 1984; 14: 440-444
    PubMedSearch in Google Scholar
  • 16 Muller YA, Ultsch MH, Kelley RF, Abraham M. de Vos. Structure of the extracellular domain of human tissue factor: location of the factor Vila binding site. Biochemistry 1994; 33: 10864-10870
    CrossrefPubMedSearch in Google Scholar
  • 17 Harlos K, Martin DMA, O’Brien DP, Jones EY, Stuart DI, Pollkarpov I, Miller A, Tuddenham EGD, Boys CWG. Crystal structure of the extracellular region of human tissue factor. Nature 1994; 370: 662-666
    CrossrefPubMedSearch in Google Scholar
  • 18 Banner DW, D’Acry A, Chene C, Winkler FK, Guha A, Konigsberg WH, Nemerson Y, Kirchhofer D. The crystal structure of the complex of blood coagulation of factor Vila with soluble tissue factor. Nature 1996; 380: 41-46
    CrossrefPubMedSearch in Google Scholar
  • 19 Kunkel TA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA 1985; 82: 488-492
    CrossrefPubMedSearch in Google Scholar
  • 20 Hartzell S, Ruder K, Langan A, Lau LF, Nathans D. A growth factor responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to human tissue factor. Mol Cell Biol 1989; 9: 2567-2573
    CrossrefPubMedSearch in Google Scholar
  • 21 Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977; 74: 5463-5467
    CrossrefPubMedSearch in Google Scholar
  • 22 Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct sensitive high-level expression of cloned genes. Mol Biol 1986; 189: 113-130
    PubMedSearch in Google Scholar
  • 23 Studier W, Rosenberg AH, Dunn JJ, Dubendorff JW. Use of the T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 1990; 185: 66-89
    PubMedSearch in Google Scholar
  • 24 Maina CV, Riggs PD, Grandea AG III, Slatko BE, Moran LS, Taglamonte JA, McReynolds LA, Guan CD. An Escherichia coli vector to express and purify foreign proteins by fusion and separation from maltose-binding protein. Gene 1988; 74: 365-373
    CrossrefPubMedSearch in Google Scholar
  • 25 Guha A, Bach R, Konigsberg W, Nemerson Y. Affinity purification of human tissue factor: Interaction of factor VII and tissue factor in detergent micelles. Proc Nat Acad Sci USA 1986; 83: 299-302
    CrossrefPubMedSearch in Google Scholar
  • 26 Waxman E, Ross JBA, Laue T, Guha A, Thiruvikraman V, Lin TC, Konigsberg WH, Nemerson Y. Tissue factor and its extracellular soluble domain: the relationship between intermolecular association with factor Vila and enzymatic activity of the complex. Biochemistry 1992; 31: 3998-4003
    CrossrefPubMedSearch in Google Scholar
  • 27 Bach R, Gentry R, Nemerson Y. Factor VII binding to tissue factor in reconstituted phospholipid vesicles: Induction of cooperativity by phos-phatidylserine. Biochemistry 1986; 25: 4007-4020
    CrossrefPubMedSearch in Google Scholar
  • 28 Ruf W, Miles D, Rehemtulla A, Edgington TS. Tissue factor residues 157167 are required for efficient proteolytic activation of factor X and factor VII. J Biol Chem 1992; 267: 22206-22210
    PubMedSearch in Google Scholar
  • 29 Stormorken H. Species differences of clotting factors in ox, dog, horse and man. Thromboplastin and Proconvertin. Acta Physiol. Scand 157 (41) 301-324
    PubMed
  • 30 Ploplis VA, Edgington TS, Fair DS. Initiation of the extrinsic of coagulation: association of factor Vila with a cell line expressing tissue factor. J Biol Chem 1987; 262: 9503-9508
    PubMedSearch in Google Scholar
  • 31 Bazan JF. Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA 1990; 87: 6934-6938
    CrossrefPubMedSearch in Google Scholar
  • 32 De Vos AM, Ultsch M, Kossiakoff AA. Human growth hormone and extracellular domain of its receptor. Crystal structure of the complex. Science 1992; 255: 306-312
    PubMedSearch in Google Scholar
  • 33 Ruf W, Schullek JR, Stone MJ, Edgington TS. Mutational mapping of functional residues in tissue factor: Identification of factor VII recognition determinants in both structural modules of the predicted cytokine receptor homology domain. Biochemistry 1994; 33: 1565-1572
    CrossrefPubMedSearch in Google Scholar
  • 34 Baron M, Main AL, Driscoll PC, Mardon HJ, Boys J, Campbell ID. (1992) H-NMR assignment and secondary structure of the cell adhesion type III module of fibronectin. Biochemistry 1992; 31: 2068-2073
    CrossrefPubMedSearch in Google Scholar
  • 35 Leahy DJ, Hendrickson WA, Aukhil I, Erickson HP. Structure of a fibronectin type III domain from tenascin phased by MAD analysis of the selenomethionyl protein. Science 1992; 258: 987-991
    CrossrefPubMedSearch in Google Scholar
  • 36 Huber AH, Wang YE, Bieber AJ, Bjorkman PJ. Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 A. Neuron 1994; 12: 717-731
    CrossrefPubMedSearch in Google Scholar
  • 37 Waxman E, Laws WR, Laue TM, Nemerson Y, Ross JBA. Human factor Vila and its complex with soluble tissue factor: evaluation of asymmetry and conformational dynamics by ultracentrifugation and fluorescence anisotropy decay methods. Biochemistry 1993; 32: 3005-3012
    CrossrefPubMedSearch in Google Scholar
  • 38 Schullek JR, Ruf W, Edgington TS. Key ligand interface residues in tissue factor contribute independently to factor Vila binding. J Biol Chem 1994; 269: 19399-19403
    PubMedSearch in Google Scholar
  • 39 Gibbs C, McCurdy S, Leung L, Paborsky L. Identification of the factor Vila binding site on tissue factor by homologous loop swap and alanine scanning mutagenesis. Biochemistry 1994; 33: 14003-14010
    CrossrefPubMedSearch in Google Scholar
  • 40 Ruf W, Kelly CR, Schullek JR, Martin DMA, Polikarpov I, Boys CWG, Tuddenham EGD, Edgington TS. Energetic contributions and topographical organization of ligand binding residues of tissue factor. Biochemistry 1995; 34: 6310-6316
    CrossrefPubMedSearch in Google Scholar
  • 41 Martin DMA, Boys CWG, Ruf W. Tissue factor: molecular recognition and cofactor function. FASEB Journal 1995; 9: 852-859
    CrossrefPubMedSearch in Google Scholar