Subscribe to RSS
Download PDF
DOI: 10.1055/s-0044-1793842
Calcium Carbonate from Anadara granosa Shells Stimulates FGF2, TGF-β1, and Collagen Type 1 Expression in Rat Dental Pulp
Authors
Abstract
Objectives Calcium carbonate (CaCO3), a major inorganic component in bones and teeth, offers potential protection against demineralization. This study investigates the effect of CaCO3 from Anadara granosa shells on the expression of fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGF-β1), and collagen type 1 in the rat dental pulp.
Materials and Methods The first maxillary molars of Rattus norvegicus were perforated and subsequently pulp capped with CaCO3 extracted from A. granosa shells. The cavities were then filled with glass ionomer cement, while the control group received calcium hydroxide (Ca(OH)2). Teeth were extracted after 7 and 14 days of treatment, and the expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp was analyzed using immunohistochemistry staining.
Results The group treated with CaCO3 from A. granosa shells exhibited significantly higher expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp at both 7 and 14 days compared with the group treated with Ca(OH)2 (p < 0.01).
Conclusion The application of CaCO3 derived from A. granosa shells enhances the proliferative phase in the dental pulp after pulp perforation and perhaps promotes reparative dentine formation.
Publication History
Article published online:
30 December 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
-
References
- 1 Al-Zahrani A, Al-Qahtani M, Al-Barti M, Bakhurji EA. Dietary determinants of dental caries prevalence and experience in Saudi schoolchildren: frequency versus quantity. ScientificWorldJournal 2022; 2022: 5447723
- 2 Anil A, Ibraheem WI, Meshni AA, Preethanath R, Anil S. Demineralization and remineralization dynamics and dental caries. In: Rusu LC, Ardelean LC. eds. Dental Caries: The Selection of Restoration Methods and Restorative Materials. London: IntechOpen; 2022
- 3 Paredes SO, da Nóbrega RF, Soares TS, Bezerra ME, de Abreu MH, Forte FD. Dental pain associated with untreated dental caries and sociodemographic factors in 5-year-old children. J Clin Exp Dent 2021; 13 (06) e552-e557
- 4 Hanna SN, Perez Alfayate R, Prichard J. Vital pulp therapy: an insight over the available literature and future expectations. Eur Endod J 2020; 5 (01) 46-53
- 5 Kratunova E, Silva D. Pulp therapy for primary and immature permanent teeth: an overview. Gen Dent 2018; 66 (06) 30-38
- 6 Bui AH, Pham KV. Evaluation of reparative dentine bridge formation after direct pulp capping with biodentine. J Int Soc Prev Community Dent 2021; 11 (01) 77-82
- 7 Álvarez-Vásquez JL, Castañeda-Alvarado CP. Dental pulp fibroblast: a star cell. J Endod 2022; 48 (08) 1005-1019
- 8 Lampiasi N. The migration and the fate of dental pulp stem cells. Biology (Basel) 2023; 12 (05) 742
- 9 Aspriyanto D, Nirwana I, Budi HS. FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract. Dent J 2018; 50 (03) 121
- 10 Vidovic-Zdrilic I, Vining KH, Vijaykumar A, Kalajzic I, Mooney DJ, Mina M. FGF2 enhances odontoblast differentiation by αSMA+ progenitors in vivo. J Dent Res 2018; 97 (10) 1170-1177
- 11 Lee E-S, Wadhwa P, Kim M-K, Bo Jiang H, Um I-W, Kim Y-M. Organic matrix of enamel and dentin and developmental defects. In: Gil de Bona A, Karaaslan H. eds. Human Tooth and Developmental Dental Defects: Compositional and Genetic Implications. London: IntechOpen; 2022
- 12 Nirwana I, Munadziroh E, Yogiartono RM, Thiyagu C, Ying CS, Dinaryanti A. Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid. J Adv Pharm Technol Res 2021; 12 (01) 27-31
- 13 Saraswati W, Dhaniar N, Wahjuningrum DA, Nuraini N, Bhardwaj A. The effect of exposure calcium carbonat from blood cockle (Anadara granosa) shells to the expression of the NF-κβ on dentin pulp complex. J Int Dent Med Res 2021; 14 (02) 549-553
- 14 Praja HA, Dhaniar N, Santoso RM. et al. Calcium carbonate of blood cockle (Anadara granosa) shells induced VEGF-A expression in dentin pulp complex: an in vivo study. Malays J Med Health Sci 2022; 18 (Suppl. 06) 24-30
- 15 Saraswati W, Soetojo A, Dhaniar N. et al. CaCO3 from Anadara granosa shell as reparative dentin inducer in odontoblast pulp cells: in-vivo study. J Oral Biol Craniofac Res 2023; 13 (02) 164-168
- 16 Trongkij P, Sutimuntanakul S, Lapthanasupkul P, Chaimanakarn C, Wong RH, Banomyong D. Pulpal responses after direct pulp capping with two calcium-silicate cements in a rat model. Dent Mater J 2019; 38 (04) 584-590
- 17 Sari RP, Revianti S, Andriani D, Prananingrum W, Rahayu RP, Sudjarwo SA. The effect of Anadara granosa shell's-Stichopus herrmanni scaffold on CD44 and IL-10 expression to decrease osteoclasts in socket healing. Eur J Dent 2021; 15 (02) 228-235
- 18 Rusdaryanti AF, Amalia U, Suharto S. Antibacterial activity of CaO from blood cockle shells (Anadara granosa) calcination against Escherichia coli . Biodiversitas (Surak) 2020; 21 (06) 2826-2830
- 19 Saharudin SH, Shariffuddin JH, Nordin NIAA. Biocomposites from (Anadara granosa) shells waste for bone material applications. IOP conf Ser Mater Sci Eng 2017; 257 (01) 012061
- 20 Zakaria MN, Pauziah NFN, Sabirin IP, Cahyanto A. Evaluation of carbonate apatite cement in inducing formation of reparative dentin in exposed dental pulp. Key Eng Mater 2017; 758: 250-254
- 21 Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci 2016; 73 (20) 3861-3885
- 22 Juniarti DE, Kunarti S, Mardiyah AA, Purniati NMD. Biomodulator of diode laser irradiation on odontoblast-like cells by expression of vascular endothelial growth factor-a and transforming growth factor-β1. Eur J Dent 2023; 17 (03) 706-712
- 23 Cobanoglu N, Alptekin T, Kitagawa H, Blatz MB, Imazato S, Ozer F. Evaluation of human pulp tissue response following direct pulp capping with a self-etching adhesive system containing MDPB. Dent Mater J 2021; 40 (03) 689-696
- 24 Zhang Y, Alexander PB, Wang X-F. TGF-β family signaling in the control of cell proliferation and survival. Cold Spring Harb Perspect Biol 2017; 9 (04) a022145
- 25 Balic A, Perver D, Pagella P. et al. Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing. Int J Oral Sci 2023; 15 (01) 30
- 26 Matsumura T, Fujimoto T, Futakuchi A. et al. TGF-β-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness. PLoS One 2020; 15 (11) e0242626
- 27 Nirwana I, Munadziroh E, Yuliati A. et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res 2022; 12 (01) 116-120
- 28 Herawati D, Pertiwi EC. Application of ozonated olive oil as adjunctive therapy after periodontal pocket curettage towards collagen density of alveolar bone in periodontitis healing process (in vivo study with Sprague Dawley). Majalah Obat Tradisional 2021; 26 (02) 129
- 29 Wulandari ER, Handajani J, Rosanto YB. Effectiveness of kirinyuh (Chromolaena odorata) extract on increasing of collagen fibers after tooth extraction. J Int Dent Med Res 2020; 13 (04) 1258-1263