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Plant Biol (Stuttg) 2001; 3(6): 616-621
DOI: 10.1055/s-2001-19365
Original Paper
Georg Thieme Verlag Stuttgart ·New York

The Description of Clones of Quercus robur L. and Q. petraea (Matt.) Liebl. with Microsatellites and AFLP in an Ancient Woodland

E. G. Bakker 1,2 , B. C. Van Dam 1 , H. J. Van Eck 2 , E. Jacobsen 2
  • 1 Department of Ecology and Environment, Alterra, AA, Wageningen, The Netherlands
  • 2 Laboratory of Plant Breeding, Wageningen University, AJ, Wageningen, The Netherlands
Further Information

Publication History

April 10, 2001

September 28, 2001

Publication Date:
02 January 2002 (online)

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Abstract

In densely populated areas autochthonous Quercus robur L. (pedunculate oak) and Q. petraea (Matt.) Liebl. (sessile oak) (Fagaceae) populations have been maintained as ancient devastated woodlands. The continuous cutting, grazing and resprouting of such woodlands has enabled the development of clonal structures. For conservation purposes, an analysis of the actual number, size and spatial distribution of clones is necessary, especially when there is an interest in genetic variation of the population. This study describes for the first time - based on microsatellite and AFLP™ analysis - clones in an autochthonous mixed Q. robur and Q. petraea population that has been coppiced and grazed for several centuries. Based on six microsatellite loci and 69 polymorphic AFLP markers, only 14 unique genotypes were detected in a plot that consisted of 80 trees. Clones were observed for both Q. robur and Q. petraea. The largest clone diameters were observed for Q. robur, with distances up to 5.8 m. The observed clone sizes may indicate the age of the trees.

Abbreviations

PVP: polyvinylpyrrolidone

PCR: polymerase chain reaction

Key words

AFLP - clones - leaf morphology - microsatellites - Quercus petraea - Quercus robur

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B. C. Van Dam

Department of Ecology and Environment
Alterra

P.O. Box 47
6700 AA Wageningen
The Netherlands

Email: b.c.vandam@alterra.wag-ur.nl

Section Editor: F. Salamini