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Horm Metab Res 2002; 34(9): 530-534
DOI: 10.1055/s-2002-34794
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Effects of 17β-Estradiol on Blood-Brain Barrier Disruption During Focal Ischemia in Rats

O.  Z.  Chi 1 , X.  Liu 1 , H.  R.  Weiss 2
  • 1Departments of Anesthesia, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA
  • 2Departments of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA
Further Information

Publication History

Received: 18 March 2002

Accepted after revision: 28 May 2002

Publication Date:
17 October 2002 (online)

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Summary

This study was performed to test whether 17β-estradiol could attenuate the blood-brain barrier disruption caused by middle cerebral artery occlusion in the ovariectomized rats. Rats aged twelve to fourteen weeks were used in this study. Their ovaries were removed one week prior to the implantation of the pellets. For the 17β-estradiol group, a 500 µg 17β-estradiol 21 day-release pellet was implanted and for the control group, a vehicle pellet was implanted 21 days before the experiments. One hour after middle cerebral artery occlusion under isoflurane anesthesia, the transfer coefficient of 14C-α-aminoisobutyric acid (104 Daltons) and the volume of 3H-dextran (70 000 Daltons) distribution were determined to represent the degree of blood-brain barrier disruption. Blood pressures and blood gases were similar between controls and 17β-estradiol-treated rats. In both groups, the transfer coefficient of the ischemic cortex was higher than that of the corresponding contralateral cortex (control: Ischemic Cortex 12.5 ± 5.9 µl/g/min, Contralateral Cortex 3.0 ± 1.6, p < 0.001; 17β-estradiol: Ischemic Cortex 6.7 ± 3.3 µl/g/min, Contralateral Cortex 2.2 ± 0.9, p < 0.01). There was no significant difference in the transfer coefficient of the contralateral cortex between these two groups. However, the transfer coefficient of the Ischemic Cortex of the 17β-estradiol-treated animals was 46 % lower than that of the control, vehicle-treated rats (p < 0.05). The increase of the volume of 3H-dextran distribution with middle cerebral artery occlusion was significant in the vehicle-treated rats (Ischemic Cortex: 6.4 ± 2.7 ml/100 g, Contralateral Cortex: 3.8 ± 0.8, p < 0.01) but not in the 17β-estradiol-treated animals. Our data suggest that chronic 17β-estradiol treatment was effective in reducing blood-brain barrier disruption during focal ischemia in the ovariectomized rats.

Key words

Brain - Capillary Permeability - Estrogen - Stroke

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O. Z. Chi, M.D.

Department of Anesthesia, University of Medicine and Dentistry of New Jersey ·

Robert Wood Johnson Medical School, 125 Paterson Street, Suite 3100 · New Brunswick · New Jersey 08901-1977 · USA ·

Phone: + 1 (732) 235-78 27

Fax: + 1 (732) 235-61 31

Email: chi@umdnj.edu