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DOI: 10.1160/TH06-01-0047
A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway
Financial support: This work was supported by funds from the National Institute of Industrial Science and Technology (AIST) to PKRK and from the New Energy and Industrial Technology Development Organization (NEDO) to HM. SCBG is supported by the Japan Society for the Promotion of Science (JSPS).Publication History
Received
24 January 2006
Accepted after revision
17 March 2006
Publication Date:
02 December 2017 (online)
Summary
A potent antidote-controlled aptamer, as an anticoagulant, has reportedly overcome the complications of acute bleeding by the administration of available anticoagulants. In the present study, we provide evidence that the aptamer binds specifically to factors IX and IXa and inhibits their functions. Furthermore, we demonstrated that the aptamer inhibits blood coagulation by interfering with the extrinsic pathway, blocking the complex of factor VIIa and tissue factor interactions with factor IX. The results from the previous and present studies suggest that the aptamer probably binds in the vicinity of the EGF1 and EGF2 domains of factor IX.
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