A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway

Subash C. B. Gopinath; Yasuo Shikamoto; Hiroshi Mizuno; Penmetcha K. R. Kumar

doi:10.1160/TH06-01-0047

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 95(05): 767-771
DOI: 10.1160/TH06-01-0047

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway

Subash C. B. Gopinath

¹Functional Nucleic Acids Group, Institute for Biological Resources and Functions

,

Yasuo Shikamoto

²Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba

,

Hiroshi Mizuno

³VALWAY Technology Center, NEC Soft Co. Ltd., Tokyo; Japan

,

Penmetcha K. R. Kumar

¹Functional Nucleic Acids Group, Institute for Biological Resources and Functions

› Author Affiliations

Abstract

Summary

A potent antidote-controlled aptamer, as an anticoagulant, has reportedly overcome the complications of acute bleeding by the administration of available anticoagulants. In the present study, we provide evidence that the aptamer binds specifically to factors IX and IXa and inhibits their functions. Furthermore, we demonstrated that the aptamer inhibits blood coagulation by interfering with the extrinsic pathway, blocking the complex of factor VIIa and tissue factor interactions with factor IX. The results from the previous and present studies suggest that the aptamer probably binds in the vicinity of the EGF1 and EGF2 domains of factor IX.

Keywords

Aptamer - blood coagulation - extrinsic and intrinsic pathway - inhibition - RNA

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References

References
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